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Quantification of the Intracellular Life Time of Water Molecules to Measure Transport Rates of Human Aquaglyceroporins

Palmgren, Madelene; Hernebring, Malin; Eriksson, Stefanie LU ; Elbing, Karin LU ; Geijer, Cecilia; Lasič, Samo; Dahl, Peter; Söndergaard Hansen, Jesper LU ; Topgaard, Daniel LU and Lindkvist-Petersson, Karin LU (2017) In Journal of Membrane Biology
Abstract

Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the... (More)

Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the intracellular life time of water, we propose that human aquaglyceroporins are poor facilitators of water and that the water transport efficiency is similar to that of passive diffusion across native cell membranes. This is distinctly different from glycerol and arsenic trioxide, where high glycerol transport efficiency was recorded.

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author
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
Aquaglyceroporin, Aquaporin, NMR, P. pastoris, Water transport
in
Journal of Membrane Biology
pages
11 pages
publisher
Springer
external identifiers
  • scopus:85029524837
  • wos:000415945900005
ISSN
0022-2631
DOI
10.1007/s00232-017-9988-4
language
English
LU publication?
yes
id
ac75f839-7f22-4cf9-9b50-d903ef3c3398
date added to LUP
2017-09-29 11:17:34
date last changed
2018-01-16 13:20:00
@article{ac75f839-7f22-4cf9-9b50-d903ef3c3398,
  abstract     = {<p>Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the intracellular life time of water, we propose that human aquaglyceroporins are poor facilitators of water and that the water transport efficiency is similar to that of passive diffusion across native cell membranes. This is distinctly different from glycerol and arsenic trioxide, where high glycerol transport efficiency was recorded.</p>},
  author       = {Palmgren, Madelene and Hernebring, Malin and Eriksson, Stefanie and Elbing, Karin and Geijer, Cecilia and Lasič, Samo and Dahl, Peter and Söndergaard Hansen, Jesper and Topgaard, Daniel and Lindkvist-Petersson, Karin},
  issn         = {0022-2631},
  keyword      = {Aquaglyceroporin,Aquaporin,NMR,P. pastoris,Water transport},
  language     = {eng},
  month        = {09},
  pages        = {11},
  publisher    = {Springer},
  series       = {Journal of Membrane Biology},
  title        = {Quantification of the Intracellular Life Time of Water Molecules to Measure Transport Rates of Human Aquaglyceroporins},
  url          = {http://dx.doi.org/10.1007/s00232-017-9988-4},
  year         = {2017},
}