Quantification of the Intracellular Life Time of Water Molecules to Measure Transport Rates of Human Aquaglyceroporins
(2017) In Journal of Membrane Biology 250(6). p.629-639- Abstract
Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the... (More)
Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the intracellular life time of water, we propose that human aquaglyceroporins are poor facilitators of water and that the water transport efficiency is similar to that of passive diffusion across native cell membranes. This is distinctly different from glycerol and arsenic trioxide, where high glycerol transport efficiency was recorded.
(Less)
- author
- Palmgren, Madelene ; Hernebring, Malin ; Eriksson, Stefanie LU ; Elbing, Karin LU ; Geijer, Cecilia ; Lasič, Samo ; Dahl, Peter ; Söndergaard Hansen, Jesper LU ; Topgaard, Daniel LU and Lindkvist-Petersson, Karin LU
- organization
- publishing date
- 2017-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aquaglyceroporin, Aquaporin, NMR, P. pastoris, Water transport
- in
- Journal of Membrane Biology
- volume
- 250
- issue
- 6
- pages
- 629 - 639
- publisher
- Springer
- external identifiers
-
- scopus:85029524837
- pmid:28914342
- wos:000415945900005
- ISSN
- 0022-2631
- DOI
- 10.1007/s00232-017-9988-4
- language
- English
- LU publication?
- yes
- id
- ac75f839-7f22-4cf9-9b50-d903ef3c3398
- date added to LUP
- 2017-09-29 11:17:34
- date last changed
- 2025-03-19 08:20:16
@article{ac75f839-7f22-4cf9-9b50-d903ef3c3398,
abstract = {{<p>Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the intracellular life time of water, we propose that human aquaglyceroporins are poor facilitators of water and that the water transport efficiency is similar to that of passive diffusion across native cell membranes. This is distinctly different from glycerol and arsenic trioxide, where high glycerol transport efficiency was recorded.</p>}},
author = {{Palmgren, Madelene and Hernebring, Malin and Eriksson, Stefanie and Elbing, Karin and Geijer, Cecilia and Lasič, Samo and Dahl, Peter and Söndergaard Hansen, Jesper and Topgaard, Daniel and Lindkvist-Petersson, Karin}},
issn = {{0022-2631}},
keywords = {{Aquaglyceroporin; Aquaporin; NMR; P. pastoris; Water transport}},
language = {{eng}},
number = {{6}},
pages = {{629--639}},
publisher = {{Springer}},
series = {{Journal of Membrane Biology}},
title = {{Quantification of the Intracellular Life Time of Water Molecules to Measure Transport Rates of Human Aquaglyceroporins}},
url = {{http://dx.doi.org/10.1007/s00232-017-9988-4}},
doi = {{10.1007/s00232-017-9988-4}},
volume = {{250}},
year = {{2017}},
}