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Affinity-bead-mediated enrichment of CD8+ lymphocytes from peripheral blood progenitor cell products using acoustophoresis

Urbansky, Anke LU ; Lenshof, Andreas LU ; Dykes, Josefina LU ; Laurell, Thomas LU and Scheding, Stefan LU (2016) In Micromachines 7(6).
Abstract

Acoustophoresis is a technique that applies ultrasonic standing wave forces in a microchannel to sort cells depending on their physical properties in relation to the surrounding media. Cell handling and separation for research and clinical applications aims to efficiently separate specific cell populations. Here, we investigated the sorting of CD8 lymphocytes from peripheral blood progenitor cell (PBPC) products by affinity-bead-mediated acoustophoresis. PBPC samples were obtained from healthy donors (n = 4) and patients (n = 18). Mononuclear cells were labeled with anti-CD8-coated magnetic beads and sorted on an acoustophoretic microfluidic device and by standard magnetic cell sorting as a reference method. CD8 lymphocytes were... (More)

Acoustophoresis is a technique that applies ultrasonic standing wave forces in a microchannel to sort cells depending on their physical properties in relation to the surrounding media. Cell handling and separation for research and clinical applications aims to efficiently separate specific cell populations. Here, we investigated the sorting of CD8 lymphocytes from peripheral blood progenitor cell (PBPC) products by affinity-bead-mediated acoustophoresis. PBPC samples were obtained from healthy donors (n = 4) and patients (n = 18). Mononuclear cells were labeled with anti-CD8-coated magnetic beads and sorted on an acoustophoretic microfluidic device and by standard magnetic cell sorting as a reference method. CD8 lymphocytes were acoustically sorted with a mean purity of 91% ± 8% and a median separation efficiency of 63% (range 15.1%-90.5%) as compared to magnetic sorting (purity 91% ± 14%, recovery 29% (range 5.1%-47.3%)). The viability as well as the proliferation capacity of sorted lymphocytes in the target fraction were unimpaired and, furthermore, hematopoietic progenitor cell assay revealed a preserved clonogenic capacity post-sorting. Bead-mediated acoustophoresis can, therefore, be utilized to efficiently sort less frequent CD8+ lymphocytes from PBPC products in a continuous flow mode while maintaining cell viability and functional capacity of both target and non-target fractions.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Acoustophoresis, CD8 lymphocytes, Cell sorting, Magnetic-beads, PBPC, Peripheral blood progenitor cells, Ultrasound
in
Micromachines
volume
7
issue
6
publisher
Multidisciplinary Digital Publishing Institute (MDPI)
external identifiers
  • scopus:84982824327
  • wos:000378733300009
ISSN
2072-666X
DOI
10.3390/mi7060101
language
English
LU publication?
yes
id
acaf2fb3-29fb-4d2f-9024-1e38c7294f2c
date added to LUP
2017-01-24 09:54:44
date last changed
2017-09-18 11:33:31
@article{acaf2fb3-29fb-4d2f-9024-1e38c7294f2c,
  abstract     = {<p>Acoustophoresis is a technique that applies ultrasonic standing wave forces in a microchannel to sort cells depending on their physical properties in relation to the surrounding media. Cell handling and separation for research and clinical applications aims to efficiently separate specific cell populations. Here, we investigated the sorting of CD8 lymphocytes from peripheral blood progenitor cell (PBPC) products by affinity-bead-mediated acoustophoresis. PBPC samples were obtained from healthy donors (n = 4) and patients (n = 18). Mononuclear cells were labeled with anti-CD8-coated magnetic beads and sorted on an acoustophoretic microfluidic device and by standard magnetic cell sorting as a reference method. CD8 lymphocytes were acoustically sorted with a mean purity of 91% ± 8% and a median separation efficiency of 63% (range 15.1%-90.5%) as compared to magnetic sorting (purity 91% ± 14%, recovery 29% (range 5.1%-47.3%)). The viability as well as the proliferation capacity of sorted lymphocytes in the target fraction were unimpaired and, furthermore, hematopoietic progenitor cell assay revealed a preserved clonogenic capacity post-sorting. Bead-mediated acoustophoresis can, therefore, be utilized to efficiently sort less frequent CD8+ lymphocytes from PBPC products in a continuous flow mode while maintaining cell viability and functional capacity of both target and non-target fractions.</p>},
  articleno    = {101},
  author       = {Urbansky, Anke and Lenshof, Andreas and Dykes, Josefina and Laurell, Thomas and Scheding, Stefan},
  issn         = {2072-666X},
  keyword      = {Acoustophoresis,CD8 lymphocytes,Cell sorting,Magnetic-beads,PBPC,Peripheral blood progenitor cells,Ultrasound},
  language     = {eng},
  month        = {06},
  number       = {6},
  publisher    = {Multidisciplinary Digital Publishing Institute (MDPI)},
  series       = {Micromachines},
  title        = {Affinity-bead-mediated enrichment of CD8+ lymphocytes from peripheral blood progenitor cell products using acoustophoresis},
  url          = {http://dx.doi.org/10.3390/mi7060101},
  volume       = {7},
  year         = {2016},
}