Adrenaline stimulates glucagon secretion in pancreatic A-cells by increasing the Ca2+ current and the number of granules close to the L-type Ca2+ channels
(1997) In Journal of General Physiology 110(3). p.217-228- Abstract
- We have monitored electrical activity, voltage-gated Ca2+ currents, and exocytosis in single rat glucagon-secreting pancreatic A-cells. The A-cells were electrically excitable and generated spontaneous Na+- and Ca2+-dependent action potentials. Under basal conditions, exocytosis was tightly linked to Ca2+ influx through omega-conotoxin-GVIA-sensitive (N-type) Ca2+ channels. Stimulation of the A-cells with adrenaline (via beta-adrenergic receptors) or forskolin produced a greater than fourfold PKA-dependent potentiation of depolarization-evoked exocytosis. This enhancement of exocytosis was due to a 50% enhancement of Ca2+ influx through L-type Ca2+ channels, an effect that accounted for <30% of the total stimulatory action. The... (More)
- We have monitored electrical activity, voltage-gated Ca2+ currents, and exocytosis in single rat glucagon-secreting pancreatic A-cells. The A-cells were electrically excitable and generated spontaneous Na+- and Ca2+-dependent action potentials. Under basal conditions, exocytosis was tightly linked to Ca2+ influx through omega-conotoxin-GVIA-sensitive (N-type) Ca2+ channels. Stimulation of the A-cells with adrenaline (via beta-adrenergic receptors) or forskolin produced a greater than fourfold PKA-dependent potentiation of depolarization-evoked exocytosis. This enhancement of exocytosis was due to a 50% enhancement of Ca2+ influx through L-type Ca2+ channels, an effect that accounted for <30% of the total stimulatory action. The remaining 70% of the stimulation was attributable to an acceleration of granule mobilization resulting in a fivefold increase in the number of readily releasable granules near the L-type Ca2+ channels. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1111594
- author
- Gromada, J ; Bokvist, K ; Ding, W G ; Barg, Sebastian LU ; Buschard, K ; Renström, Erik LU and Rorsman, Patrik LU
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- glucagon, Ca2+, secretion
- in
- Journal of General Physiology
- volume
- 110
- issue
- 3
- pages
- 217 - 228
- publisher
- Rockefeller Institute for Medical Research
- external identifiers
-
- pmid:9276750
- scopus:0030967933
- ISSN
- 0022-1295
- language
- English
- LU publication?
- no
- id
- ad7f60ac-295c-4a20-9607-523430576791 (old id 1111594)
- alternative location
- http://www.jgp.org/cgi/content/full/110/3/217
- date added to LUP
- 2016-04-01 15:48:33
- date last changed
- 2022-04-22 17:33:07
@article{ad7f60ac-295c-4a20-9607-523430576791, abstract = {{We have monitored electrical activity, voltage-gated Ca2+ currents, and exocytosis in single rat glucagon-secreting pancreatic A-cells. The A-cells were electrically excitable and generated spontaneous Na+- and Ca2+-dependent action potentials. Under basal conditions, exocytosis was tightly linked to Ca2+ influx through omega-conotoxin-GVIA-sensitive (N-type) Ca2+ channels. Stimulation of the A-cells with adrenaline (via beta-adrenergic receptors) or forskolin produced a greater than fourfold PKA-dependent potentiation of depolarization-evoked exocytosis. This enhancement of exocytosis was due to a 50% enhancement of Ca2+ influx through L-type Ca2+ channels, an effect that accounted for <30% of the total stimulatory action. The remaining 70% of the stimulation was attributable to an acceleration of granule mobilization resulting in a fivefold increase in the number of readily releasable granules near the L-type Ca2+ channels.}}, author = {{Gromada, J and Bokvist, K and Ding, W G and Barg, Sebastian and Buschard, K and Renström, Erik and Rorsman, Patrik}}, issn = {{0022-1295}}, keywords = {{glucagon; Ca2+; secretion}}, language = {{eng}}, number = {{3}}, pages = {{217--228}}, publisher = {{Rockefeller Institute for Medical Research}}, series = {{Journal of General Physiology}}, title = {{Adrenaline stimulates glucagon secretion in pancreatic A-cells by increasing the Ca2+ current and the number of granules close to the L-type Ca2+ channels}}, url = {{http://www.jgp.org/cgi/content/full/110/3/217}}, volume = {{110}}, year = {{1997}}, }