Evaluation of progesterone content in saliva using magnetic particle-based immuno supported liquid membrane assay (m-ISLMA)
(2006) In Biosensors & Bioelectronics 22(2). p.241-246- Abstract
- Progesterone in saliva was monitored using a new method called magnetic particle-based immuno supported liquid membrane assay (m-ISLMA) in a sequential injection (SI) setup, allowing automatic sample cleanup, analyte enrichment, and detection in a single analysis unit. Progesterone (Ag) diffuses from a continuous flowing sample - the donor - into a supported organic liquid membrane (SLM), based on analyte partitioning (solubility) between the aqueous donor and the organic phase. The Ag is re-extracted from the SLM into a second stagnant aqueous acceptor, containing antibodies (Ab) immobilized on magnetic beads, held at the bottom of the acceptor by a magnet. Due to the formation of strong Ag-Ab-bead complexes and a large excess of... (More)
- Progesterone in saliva was monitored using a new method called magnetic particle-based immuno supported liquid membrane assay (m-ISLMA) in a sequential injection (SI) setup, allowing automatic sample cleanup, analyte enrichment, and detection in a single analysis unit. Progesterone (Ag) diffuses from a continuous flowing sample - the donor - into a supported organic liquid membrane (SLM), based on analyte partitioning (solubility) between the aqueous donor and the organic phase. The Ag is re-extracted from the SLM into a second stagnant aqueous acceptor, containing antibodies (Ab) immobilized on magnetic beads, held at the bottom of the acceptor by a magnet. Due to the formation of strong Ag-Ab-bead complexes and a large excess of Ab-beads, the Ag is accumulated and selectively enriched in the acceptor. The extracted progesterone was quantified by injecting into the acceptor a horseradish peroxidase (HRP) labeled analyte tracer, the substrate (luminol, H2O2, and p-iodophenol), and finally detection of the generated chemiluminescence by a photomultiplier tube. After optimization of experimental parameters (e.g., sample flow rate, extraction time, type of organic solvent and antibody-bead concentration in the acceptor), a detection limit of 8.50 +/- 0.17 fg L-1 and a dynamic range between 35 fg L-1 and 10 pg L-1 was reached. The progesterone level of saliva for three subjects (women in different period of ovarian cycle) was investigated, and the corresponding progesterone concentrations detected with m-ISLMA coincided well with the expected values. (c) 2006 Elsevier B.V. All rights reserved. (Less)
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https://lup.lub.lu.se/record/389767
- author
- Tudorache, Madalina LU ; Zdrojewska, Izabela Anna and Emnéus, Jenny LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- magnetic particle-based immuno supported liquid membrane assay, (m-ISLMA), saliva, progesterone
- in
- Biosensors & Bioelectronics
- volume
- 22
- issue
- 2
- pages
- 241 - 246
- publisher
- Elsevier
- external identifiers
-
- wos:000240906100012
- scopus:33747334644
- pmid:16473507
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2006.01.002
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- adb67960-e0b1-449d-b05e-375bb159655a (old id 389767)
- date added to LUP
- 2016-04-01 16:31:38
- date last changed
- 2024-07-02 13:21:03
@article{adb67960-e0b1-449d-b05e-375bb159655a,
abstract = {{Progesterone in saliva was monitored using a new method called magnetic particle-based immuno supported liquid membrane assay (m-ISLMA) in a sequential injection (SI) setup, allowing automatic sample cleanup, analyte enrichment, and detection in a single analysis unit. Progesterone (Ag) diffuses from a continuous flowing sample - the donor - into a supported organic liquid membrane (SLM), based on analyte partitioning (solubility) between the aqueous donor and the organic phase. The Ag is re-extracted from the SLM into a second stagnant aqueous acceptor, containing antibodies (Ab) immobilized on magnetic beads, held at the bottom of the acceptor by a magnet. Due to the formation of strong Ag-Ab-bead complexes and a large excess of Ab-beads, the Ag is accumulated and selectively enriched in the acceptor. The extracted progesterone was quantified by injecting into the acceptor a horseradish peroxidase (HRP) labeled analyte tracer, the substrate (luminol, H2O2, and p-iodophenol), and finally detection of the generated chemiluminescence by a photomultiplier tube. After optimization of experimental parameters (e.g., sample flow rate, extraction time, type of organic solvent and antibody-bead concentration in the acceptor), a detection limit of 8.50 +/- 0.17 fg L-1 and a dynamic range between 35 fg L-1 and 10 pg L-1 was reached. The progesterone level of saliva for three subjects (women in different period of ovarian cycle) was investigated, and the corresponding progesterone concentrations detected with m-ISLMA coincided well with the expected values. (c) 2006 Elsevier B.V. All rights reserved.}},
author = {{Tudorache, Madalina and Zdrojewska, Izabela Anna and Emnéus, Jenny}},
issn = {{1873-4235}},
keywords = {{magnetic particle-based immuno supported liquid membrane assay; (m-ISLMA); saliva; progesterone}},
language = {{eng}},
number = {{2}},
pages = {{241--246}},
publisher = {{Elsevier}},
series = {{Biosensors & Bioelectronics}},
title = {{Evaluation of progesterone content in saliva using magnetic particle-based immuno supported liquid membrane assay (m-ISLMA)}},
url = {{http://dx.doi.org/10.1016/j.bios.2006.01.002}},
doi = {{10.1016/j.bios.2006.01.002}},
volume = {{22}},
year = {{2006}},
}