Importance of the 3' untranslated region of ornithine decarboxylase mRNA in the translational regulation of the enzyme
(2001) In The Biochemical journal 356(2). p.34-627- Abstract
Translational regulation of ornithine decarboxylase (ODC), which catalyses the first step in the biosynthesis of polyamines, appears to be an important mechanism in the strong feedback control as well as in the hypotonic induction of the enzyme. However, the exact mechanisms are not yet understood. The ODC mRNA has long 5' and 3' untranslated regions (UTRs) which may be involved in the translational control of the enzyme. In the present study we have used a series of stable transfectants of Chinese Hamster ovary cells expressing ODC mRNAs with various truncations in the 5' and 3' UTRs to investigate the importance of these regions. It is demonstrated that neither the 5' UTR nor the 3' UTR appears to be involved in the polyamine-mediated... (More)
Translational regulation of ornithine decarboxylase (ODC), which catalyses the first step in the biosynthesis of polyamines, appears to be an important mechanism in the strong feedback control as well as in the hypotonic induction of the enzyme. However, the exact mechanisms are not yet understood. The ODC mRNA has long 5' and 3' untranslated regions (UTRs) which may be involved in the translational control of the enzyme. In the present study we have used a series of stable transfectants of Chinese Hamster ovary cells expressing ODC mRNAs with various truncations in the 5' and 3' UTRs to investigate the importance of these regions. It is demonstrated that neither the 5' UTR nor the 3' UTR appears to be involved in the polyamine-mediated feedback control of ODC synthesis. The hypotonic induction of ODC, on the other hand, was shown to be highly dependent on the presence of the 3' UTR, but not on the 5' UTR, of ODC mRNA. Cells expressing ODC mRNAs lacking the 3' UTR showed no, or only a very slight, induction of ODC whether the 5' UTR was present or not, whereas the cell lines expressing ODC mRNAs containing the 3' UTR (with or without the 5' UTR) markedly induced ODC after a hypotonic shock. The present finding of a role for the ODC mRNA 3' UTR in the hypotonic induction of ODC is the first demonstration of a specific effect of the 3' UTR in the regulation of ODC.
(Less)
- author
- Lövkvist Wallström, E LU ; Takao, K ; Wendt, A LU ; Vargiu, C ; Yin, H and Persson, Lo LU
- organization
- publishing date
- 2001-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 3' Untranslated Regions, 5' Untranslated Regions, Animals, CHO Cells, Cricetinae, Down-Regulation/drug effects, Enzyme Induction, Hypotonic Solutions, Ornithine Decarboxylase/biosynthesis, Protein Biosynthesis, Putrescine/metabolism, RNA, Messenger/genetics, Spermidine/metabolism, Spermine/metabolism
- in
- The Biochemical journal
- volume
- 356
- issue
- 2
- pages
- 8 pages
- publisher
- Portland Press
- external identifiers
-
- pmid:11368794
- scopus:0035354256
- ISSN
- 0264-6021
- DOI
- 10.1042/bj3560627
- language
- English
- LU publication?
- yes
- id
- af06cfda-2322-4b6e-b16d-d7310436c57b
- date added to LUP
- 2019-06-20 11:35:25
- date last changed
- 2025-01-09 15:26:06
@article{af06cfda-2322-4b6e-b16d-d7310436c57b, abstract = {{<p>Translational regulation of ornithine decarboxylase (ODC), which catalyses the first step in the biosynthesis of polyamines, appears to be an important mechanism in the strong feedback control as well as in the hypotonic induction of the enzyme. However, the exact mechanisms are not yet understood. The ODC mRNA has long 5' and 3' untranslated regions (UTRs) which may be involved in the translational control of the enzyme. In the present study we have used a series of stable transfectants of Chinese Hamster ovary cells expressing ODC mRNAs with various truncations in the 5' and 3' UTRs to investigate the importance of these regions. It is demonstrated that neither the 5' UTR nor the 3' UTR appears to be involved in the polyamine-mediated feedback control of ODC synthesis. The hypotonic induction of ODC, on the other hand, was shown to be highly dependent on the presence of the 3' UTR, but not on the 5' UTR, of ODC mRNA. Cells expressing ODC mRNAs lacking the 3' UTR showed no, or only a very slight, induction of ODC whether the 5' UTR was present or not, whereas the cell lines expressing ODC mRNAs containing the 3' UTR (with or without the 5' UTR) markedly induced ODC after a hypotonic shock. The present finding of a role for the ODC mRNA 3' UTR in the hypotonic induction of ODC is the first demonstration of a specific effect of the 3' UTR in the regulation of ODC.</p>}}, author = {{Lövkvist Wallström, E and Takao, K and Wendt, A and Vargiu, C and Yin, H and Persson, Lo}}, issn = {{0264-6021}}, keywords = {{3' Untranslated Regions; 5' Untranslated Regions; Animals; CHO Cells; Cricetinae; Down-Regulation/drug effects; Enzyme Induction; Hypotonic Solutions; Ornithine Decarboxylase/biosynthesis; Protein Biosynthesis; Putrescine/metabolism; RNA, Messenger/genetics; Spermidine/metabolism; Spermine/metabolism}}, language = {{eng}}, number = {{2}}, pages = {{34--627}}, publisher = {{Portland Press}}, series = {{The Biochemical journal}}, title = {{Importance of the 3' untranslated region of ornithine decarboxylase mRNA in the translational regulation of the enzyme}}, url = {{http://dx.doi.org/10.1042/bj3560627}}, doi = {{10.1042/bj3560627}}, volume = {{356}}, year = {{2001}}, }