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Importance of the 3' untranslated region of ornithine decarboxylase mRNA in the translational regulation of the enzyme

Lövkvist Wallström, E LU ; Takao, K ; Wendt, A LU ; Vargiu, C ; Yin, H and Persson, Lo LU (2001) In The Biochemical journal 356(2). p.34-627
Abstract

Translational regulation of ornithine decarboxylase (ODC), which catalyses the first step in the biosynthesis of polyamines, appears to be an important mechanism in the strong feedback control as well as in the hypotonic induction of the enzyme. However, the exact mechanisms are not yet understood. The ODC mRNA has long 5' and 3' untranslated regions (UTRs) which may be involved in the translational control of the enzyme. In the present study we have used a series of stable transfectants of Chinese Hamster ovary cells expressing ODC mRNAs with various truncations in the 5' and 3' UTRs to investigate the importance of these regions. It is demonstrated that neither the 5' UTR nor the 3' UTR appears to be involved in the polyamine-mediated... (More)

Translational regulation of ornithine decarboxylase (ODC), which catalyses the first step in the biosynthesis of polyamines, appears to be an important mechanism in the strong feedback control as well as in the hypotonic induction of the enzyme. However, the exact mechanisms are not yet understood. The ODC mRNA has long 5' and 3' untranslated regions (UTRs) which may be involved in the translational control of the enzyme. In the present study we have used a series of stable transfectants of Chinese Hamster ovary cells expressing ODC mRNAs with various truncations in the 5' and 3' UTRs to investigate the importance of these regions. It is demonstrated that neither the 5' UTR nor the 3' UTR appears to be involved in the polyamine-mediated feedback control of ODC synthesis. The hypotonic induction of ODC, on the other hand, was shown to be highly dependent on the presence of the 3' UTR, but not on the 5' UTR, of ODC mRNA. Cells expressing ODC mRNAs lacking the 3' UTR showed no, or only a very slight, induction of ODC whether the 5' UTR was present or not, whereas the cell lines expressing ODC mRNAs containing the 3' UTR (with or without the 5' UTR) markedly induced ODC after a hypotonic shock. The present finding of a role for the ODC mRNA 3' UTR in the hypotonic induction of ODC is the first demonstration of a specific effect of the 3' UTR in the regulation of ODC.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
3' Untranslated Regions, 5' Untranslated Regions, Animals, CHO Cells, Cricetinae, Down-Regulation/drug effects, Enzyme Induction, Hypotonic Solutions, Ornithine Decarboxylase/biosynthesis, Protein Biosynthesis, Putrescine/metabolism, RNA, Messenger/genetics, Spermidine/metabolism, Spermine/metabolism
in
The Biochemical journal
volume
356
issue
2
pages
8 pages
publisher
Portland Press
external identifiers
  • scopus:0035354256
  • pmid:11368794
ISSN
0264-6021
DOI
10.1042/bj3560627
language
English
LU publication?
yes
id
af06cfda-2322-4b6e-b16d-d7310436c57b
date added to LUP
2019-06-20 11:35:25
date last changed
2021-02-17 05:36:17
@article{af06cfda-2322-4b6e-b16d-d7310436c57b,
  abstract     = {<p>Translational regulation of ornithine decarboxylase (ODC), which catalyses the first step in the biosynthesis of polyamines, appears to be an important mechanism in the strong feedback control as well as in the hypotonic induction of the enzyme. However, the exact mechanisms are not yet understood. The ODC mRNA has long 5' and 3' untranslated regions (UTRs) which may be involved in the translational control of the enzyme. In the present study we have used a series of stable transfectants of Chinese Hamster ovary cells expressing ODC mRNAs with various truncations in the 5' and 3' UTRs to investigate the importance of these regions. It is demonstrated that neither the 5' UTR nor the 3' UTR appears to be involved in the polyamine-mediated feedback control of ODC synthesis. The hypotonic induction of ODC, on the other hand, was shown to be highly dependent on the presence of the 3' UTR, but not on the 5' UTR, of ODC mRNA. Cells expressing ODC mRNAs lacking the 3' UTR showed no, or only a very slight, induction of ODC whether the 5' UTR was present or not, whereas the cell lines expressing ODC mRNAs containing the 3' UTR (with or without the 5' UTR) markedly induced ODC after a hypotonic shock. The present finding of a role for the ODC mRNA 3' UTR in the hypotonic induction of ODC is the first demonstration of a specific effect of the 3' UTR in the regulation of ODC.</p>},
  author       = {Lövkvist Wallström, E and Takao, K and Wendt, A and Vargiu, C and Yin, H and Persson, Lo},
  issn         = {0264-6021},
  language     = {eng},
  number       = {2},
  pages        = {34--627},
  publisher    = {Portland Press},
  series       = {The Biochemical journal},
  title        = {Importance of the 3' untranslated region of ornithine decarboxylase mRNA in the translational regulation of the enzyme},
  url          = {http://dx.doi.org/10.1042/bj3560627},
  doi          = {10.1042/bj3560627},
  volume       = {356},
  year         = {2001},
}