Two-stage diffuse fluorescence tomography for monitoring of drug distribution in photodynamic therapy of tumors
Šušnjar, Stefan LU
; Ghauri, Muhammad Daniyal
; Thomasson, Björn
; Sekar, Sanathana Konugolu Venkata
; Andersson-Engels, Stefan
; Swartling, Johannes
and Reistad, Nina
LU
(2025)
In Journal of Biomedical Optics
30(1).
- Abstract
- Significance
The spatial distribution of the photosensitizing drug concentration is an important parameter for predicting the photodynamic therapy (PDT) outcome. Current diffuse fluorescence tomography methods lack accuracy in quantifying drug concentration. The development of accurate methods for monitoring the temporal evolution of the drug distribution in tissue can advance the real-time light dosimetry in PDT of tumors, leading to better treatment outcomes.
Aim
We develop diffuse optical tomography methods based on interstitial fluorescence measurements to accurately reconstruct the spatial distribution of fluorescent photosensitizing drugs in real-time.
Approach
A two-stage reconstruction algorithm is... (More) - Significance
The spatial distribution of the photosensitizing drug concentration is an important parameter for predicting the photodynamic therapy (PDT) outcome. Current diffuse fluorescence tomography methods lack accuracy in quantifying drug concentration. The development of accurate methods for monitoring the temporal evolution of the drug distribution in tissue can advance the real-time light dosimetry in PDT of tumors, leading to better treatment outcomes.
Aim
We develop diffuse optical tomography methods based on interstitial fluorescence measurements to accurately reconstruct the spatial distribution of fluorescent photosensitizing drugs in real-time.
Approach
A two-stage reconstruction algorithm is proposed. The capabilities and limitations of this method are studied in various simulated scenarios. For the first time, experimental validation is conducted using the clinical system for interstitial PDT of prostate cancer on prostate tissue-mimicking phantoms with the photosensitizer verteporfin.
Results
The average relative error of the reconstructed fluorophore absorption was less than 10%, whereas the fluorescent inclusion reconstructed volume relative error was less than 35%.
Conclusions
The proposed method can be used to monitor the temporal evolution of the photosensitizing drug concentration in tumor tissue during photodynamic therapy. This is an important step forward in the development of the next generation of real-time light dosimetry algorithms for photodynamic therapy. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/af540350-f191-426a-9630-9af742fd6b19
- author
- Šušnjar, Stefan
LU
; Ghauri, Muhammad Daniyal
; Thomasson, Björn
; Sekar, Sanathana Konugolu Venkata
; Andersson-Engels, Stefan
; Swartling, Johannes
and Reistad, Nina
LU
- organization
- publishing date
- 2025-01-30
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- diffuse optical tomography, fluorescence, photodynamic therapy, photo sensitizing drug, inverse problem, tissue phantoms
- in
- Journal of Biomedical Optics
- volume
- 30
- issue
- 1
- pages
- 26 pages
- publisher
- SPIE
- external identifiers
-
- scopus:85217357153
- pmid:39885926
- ISSN
- 1083-3668
- DOI
- 10.1117/1.JBO.30.1.015003
- language
- English
- LU publication?
- yes
- id
- af540350-f191-426a-9630-9af742fd6b19
- date added to LUP
- 2025-01-30 19:07:40
- date last changed
- 2025-05-02 03:00:13
@article{af540350-f191-426a-9630-9af742fd6b19,
abstract = {{Significance<br/>The spatial distribution of the photosensitizing drug concentration is an important parameter for predicting the photodynamic therapy (PDT) outcome. Current diffuse fluorescence tomography methods lack accuracy in quantifying drug concentration. The development of accurate methods for monitoring the temporal evolution of the drug distribution in tissue can advance the real-time light dosimetry in PDT of tumors, leading to better treatment outcomes.<br/><br/>Aim<br/>We develop diffuse optical tomography methods based on interstitial fluorescence measurements to accurately reconstruct the spatial distribution of fluorescent photosensitizing drugs in real-time.<br/><br/>Approach<br/>A two-stage reconstruction algorithm is proposed. The capabilities and limitations of this method are studied in various simulated scenarios. For the first time, experimental validation is conducted using the clinical system for interstitial PDT of prostate cancer on prostate tissue-mimicking phantoms with the photosensitizer verteporfin.<br/><br/>Results<br/>The average relative error of the reconstructed fluorophore absorption was less than 10%, whereas the fluorescent inclusion reconstructed volume relative error was less than 35%.<br/><br/>Conclusions<br/>The proposed method can be used to monitor the temporal evolution of the photosensitizing drug concentration in tumor tissue during photodynamic therapy. This is an important step forward in the development of the next generation of real-time light dosimetry algorithms for photodynamic therapy.}},
author = {{Šušnjar, Stefan and Ghauri, Muhammad Daniyal and Thomasson, Björn and Sekar, Sanathana Konugolu Venkata and Andersson-Engels, Stefan and Swartling, Johannes and Reistad, Nina}},
issn = {{1083-3668}},
keywords = {{diffuse optical tomography; fluorescence; photodynamic therapy; photo sensitizing drug; inverse problem; tissue phantoms}},
language = {{eng}},
month = {{01}},
number = {{1}},
publisher = {{SPIE}},
series = {{Journal of Biomedical Optics}},
title = {{Two-stage diffuse fluorescence tomography for monitoring of drug distribution in photodynamic therapy of tumors}},
url = {{http://dx.doi.org/10.1117/1.JBO.30.1.015003}},
doi = {{10.1117/1.JBO.30.1.015003}},
volume = {{30}},
year = {{2025}},
}