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HLA genotypes in coeliac disease and healthy individuals carrying gliadin antibodies

Pettersson, Anna ; Sjöberg, Klas LU orcid ; Lernmark, Åke LU orcid and Eriksson, Sten LU (1993) In European Journal of Gastroenterology and Hepatology 5(6). p.445-450
Abstract

Objective: Coeliac disease is closely associated with the presence of gliadin antibodies and certain HLA class II alleles. The aim of this study was to compare the HLA genotype in gliadin antibody-positive individuals without coeliac disease with that in patients with clinically verified coeliac disease.Design: HLA genotyping was carried out in 65 patients with coeliac disease and the results were compared with those from 19 gliadin antibody-positive and 96 antibody-negative healthy blood donors.Methods: Immunoglobulin G and A gliadin antibodies were measured with a micro-enzyme-linked immunosorbent assay technique. Restriction fragment length polymorphism typing for determination of HLA was carried out by means of Southern blot... (More)

Objective: Coeliac disease is closely associated with the presence of gliadin antibodies and certain HLA class II alleles. The aim of this study was to compare the HLA genotype in gliadin antibody-positive individuals without coeliac disease with that in patients with clinically verified coeliac disease.Design: HLA genotyping was carried out in 65 patients with coeliac disease and the results were compared with those from 19 gliadin antibody-positive and 96 antibody-negative healthy blood donors.Methods: Immunoglobulin G and A gliadin antibodies were measured with a micro-enzyme-linked immunosorbent assay technique. Restriction fragment length polymorphism typing for determination of HLA was carried out by means of Southern blot analysis of restriction digests.Results: Coeliac disease was significantly correlated with the presence of HLA-DR3 and DQ2, whereas DQ1, DQ7, DR1, DR4 and DR5 were all under-represented in coeliac disease patients. Of the extended haplotypes, only DR3-DQ2 was associated with the disease, while DR1-DQ1, DR4-DQ7 and DR5-DQ7 were under-represented. The correlation between DR3-DQ2 and coeliac disease depended on homozygosity; 29% of the patients and 4% of the controls were homozygous for DQ2 compared with 18 and 1%, respectively, for DR3. Only five out of 19 of the gliadin antibody-positive healthy blood donors had HLA genotypes characteristic of coeliac disease, while 15 were DQ1-positive which would be expected to confer protection.Conclusion: These findings suggest that the presence or development of gliadin antibodies is independent of the HLA genotype.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Coeliac disease, Gliadin antibodies, HLA genotype
in
European Journal of Gastroenterology and Hepatology
volume
5
issue
6
pages
6 pages
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0027233432
ISSN
0954-691X
DOI
10.1097/00042737-199306000-00009
language
English
LU publication?
yes
id
af985591-96c2-42bf-9aed-3e85745229dc
date added to LUP
2019-09-11 09:29:55
date last changed
2023-04-10 00:04:30
@article{af985591-96c2-42bf-9aed-3e85745229dc,
  abstract     = {{<p>Objective: Coeliac disease is closely associated with the presence of gliadin antibodies and certain HLA class II alleles. The aim of this study was to compare the HLA genotype in gliadin antibody-positive individuals without coeliac disease with that in patients with clinically verified coeliac disease.Design: HLA genotyping was carried out in 65 patients with coeliac disease and the results were compared with those from 19 gliadin antibody-positive and 96 antibody-negative healthy blood donors.Methods: Immunoglobulin G and A gliadin antibodies were measured with a micro-enzyme-linked immunosorbent assay technique. Restriction fragment length polymorphism typing for determination of HLA was carried out by means of Southern blot analysis of restriction digests.Results: Coeliac disease was significantly correlated with the presence of HLA-DR3 and DQ2, whereas DQ1, DQ7, DR1, DR4 and DR5 were all under-represented in coeliac disease patients. Of the extended haplotypes, only DR3-DQ2 was associated with the disease, while DR1-DQ1, DR4-DQ7 and DR5-DQ7 were under-represented. The correlation between DR3-DQ2 and coeliac disease depended on homozygosity; 29% of the patients and 4% of the controls were homozygous for DQ2 compared with 18 and 1%, respectively, for DR3. Only five out of 19 of the gliadin antibody-positive healthy blood donors had HLA genotypes characteristic of coeliac disease, while 15 were DQ1-positive which would be expected to confer protection.Conclusion: These findings suggest that the presence or development of gliadin antibodies is independent of the HLA genotype.</p>}},
  author       = {{Pettersson, Anna and Sjöberg, Klas and Lernmark, Åke and Eriksson, Sten}},
  issn         = {{0954-691X}},
  keywords     = {{Coeliac disease; Gliadin antibodies; HLA genotype}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{6}},
  pages        = {{445--450}},
  publisher    = {{Lippincott Williams & Wilkins}},
  series       = {{European Journal of Gastroenterology and Hepatology}},
  title        = {{HLA genotypes in coeliac disease and healthy individuals carrying gliadin antibodies}},
  url          = {{http://dx.doi.org/10.1097/00042737-199306000-00009}},
  doi          = {{10.1097/00042737-199306000-00009}},
  volume       = {{5}},
  year         = {{1993}},
}