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Polymer–polymer organic solvent two‐phase system : A new type of reaction medium for bioorganic synthesis

Otamiri, Marina ; Adlercreutz, Patrick LU and Mattiasson, Bo LU (1994) In Biotechnology and Bioengineering 43(10). p.987-994
Abstract

Mixing solutions of polymers dissolved in chloroform resulted in turbid solutions that parted into two separate phases upon standing. Each phase consisted primarily of one of the two polymers and contained only small amounts of the other. An enzyme (α‐chymotrypsin) added to the two‐phase system partitioned preferentially to one of the phases; this was observed with native enzyme and with enzyme associated with one of the polymers through non‐convalent interactions. Under the conditions studied, α‐chymotrypsin was active and expressed even higher activity and stability than native enzyme added to the organic solvent without polymer. An emulsion was easily formed on mixing with small droplets of one of the phases suspended in the other... (More)

Mixing solutions of polymers dissolved in chloroform resulted in turbid solutions that parted into two separate phases upon standing. Each phase consisted primarily of one of the two polymers and contained only small amounts of the other. An enzyme (α‐chymotrypsin) added to the two‐phase system partitioned preferentially to one of the phases; this was observed with native enzyme and with enzyme associated with one of the polymers through non‐convalent interactions. Under the conditions studied, α‐chymotrypsin was active and expressed even higher activity and stability than native enzyme added to the organic solvent without polymer. An emulsion was easily formed on mixing with small droplets of one of the phases suspended in the other phase. By operating with the enzyme in the emulsion, a very attractive system for carrying out enzyme‐catalyzed conversions was created. Short diffusion distances and minimized steric hindrance are two characteristics of such systems. At the conclusion of the reaction, stirring/mixing was ceased and, after phase separation, it was possible to recover the enzyme as well as the product, under ideal conditins, from different phases. The enzyme was then reused. © 1994 John Wiley & Sons, Inc.

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publication status
published
subject
keywords
bioorganic synthesis, enzymes, polymers
in
Biotechnology and Bioengineering
volume
43
issue
10
pages
8 pages
publisher
John Wiley and Sons Inc.
external identifiers
  • scopus:0028408964
ISSN
0006-3592
DOI
10.1002/bit.260431012
language
English
LU publication?
yes
id
b0563957-a15e-4558-a317-c2c43d09baea
date added to LUP
2019-06-22 09:13:14
date last changed
2020-01-13 02:04:54
@article{b0563957-a15e-4558-a317-c2c43d09baea,
  abstract     = {<p>Mixing solutions of polymers dissolved in chloroform resulted in turbid solutions that parted into two separate phases upon standing. Each phase consisted primarily of one of the two polymers and contained only small amounts of the other. An enzyme (α‐chymotrypsin) added to the two‐phase system partitioned preferentially to one of the phases; this was observed with native enzyme and with enzyme associated with one of the polymers through non‐convalent interactions. Under the conditions studied, α‐chymotrypsin was active and expressed even higher activity and stability than native enzyme added to the organic solvent without polymer. An emulsion was easily formed on mixing with small droplets of one of the phases suspended in the other phase. By operating with the enzyme in the emulsion, a very attractive system for carrying out enzyme‐catalyzed conversions was created. Short diffusion distances and minimized steric hindrance are two characteristics of such systems. At the conclusion of the reaction, stirring/mixing was ceased and, after phase separation, it was possible to recover the enzyme as well as the product, under ideal conditins, from different phases. The enzyme was then reused. © 1994 John Wiley &amp; Sons, Inc.</p>},
  author       = {Otamiri, Marina and Adlercreutz, Patrick and Mattiasson, Bo},
  issn         = {0006-3592},
  language     = {eng},
  month        = {01},
  number       = {10},
  pages        = {987--994},
  publisher    = {John Wiley and Sons Inc.},
  series       = {Biotechnology and Bioengineering},
  title        = {Polymer–polymer organic solvent two‐phase system : A new type of reaction medium for bioorganic synthesis},
  url          = {http://dx.doi.org/10.1002/bit.260431012},
  doi          = {10.1002/bit.260431012},
  volume       = {43},
  year         = {1994},
}