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Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women

van den Berg, Caroline B.; Duvekot, Johannes J.; Güzel, Coşkun; Hansson, Stefan LU ; de Leeuw, Thomas G.; Steegers, Eric A P; Versendaal, Johannes; Luider, Theo M. and Stoop, Marcel P. (2017) In Proteomics - Clinical Applications 11(1-2).
Abstract

Purpose: To investigate the cerebrospinal fluid (CSF) proteome of patients with preeclampsia (PE) and normotensive pregnant women, in order to provide a better understanding of brain involvement in PE. Experimental design: Ninety-eight CSF samples (43 women with PE and 55 normotensive controls) were analyzed by LC–MS/MS proteome profiling. CSF was obtained during the spinal puncture before caesarean delivery. Results: Eight proteins were higher abundant and 17 proteins were lower abundant in patients with PE. The most significantly differentially abundant protein was protein AMBP (alpha-1-microglobulin/bikunin precursor). This finding was validated by performing an ELISA experiment (p = 0.002). Conclusions and clinical relevance: The... (More)

Purpose: To investigate the cerebrospinal fluid (CSF) proteome of patients with preeclampsia (PE) and normotensive pregnant women, in order to provide a better understanding of brain involvement in PE. Experimental design: Ninety-eight CSF samples (43 women with PE and 55 normotensive controls) were analyzed by LC–MS/MS proteome profiling. CSF was obtained during the spinal puncture before caesarean delivery. Results: Eight proteins were higher abundant and 17 proteins were lower abundant in patients with PE. The most significantly differentially abundant protein was protein AMBP (alpha-1-microglobulin/bikunin precursor). This finding was validated by performing an ELISA experiment (p = 0.002). Conclusions and clinical relevance: The current study showed a clear difference between the protein profiles of CSF from patients with PE and normotensive pregnant women. Protein AMBP is a precursor of a heme-binding protein that counteracts the damaging effects of free hemoglobin, which may be related to the presence of free hemoglobin in CSF. Protein levels showed correlations with clinical symptoms during pregnancy and postpartum. To our knowledge, this is the first LC–MS/MS proteome profiling study on a unique set of CSF samples from (severe) preeclamptic patients and normotensive pregnant women.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Blood–brain barrier, Cerebrospinal fluid, Preeclampsia, Pregnancy, Protein AMBP
in
Proteomics - Clinical Applications
volume
11
issue
1-2
publisher
John Wiley & Sons
external identifiers
  • scopus:85001124045
  • wos:000396394800005
ISSN
1862-8346
DOI
10.1002/prca.201600082
language
English
LU publication?
yes
id
b07b11f0-fcbd-454c-9e64-39f475ed485c
date added to LUP
2017-03-23 09:25:13
date last changed
2018-01-07 11:56:45
@article{b07b11f0-fcbd-454c-9e64-39f475ed485c,
  abstract     = {<p>Purpose: To investigate the cerebrospinal fluid (CSF) proteome of patients with preeclampsia (PE) and normotensive pregnant women, in order to provide a better understanding of brain involvement in PE. Experimental design: Ninety-eight CSF samples (43 women with PE and 55 normotensive controls) were analyzed by LC–MS/MS proteome profiling. CSF was obtained during the spinal puncture before caesarean delivery. Results: Eight proteins were higher abundant and 17 proteins were lower abundant in patients with PE. The most significantly differentially abundant protein was protein AMBP (alpha-1-microglobulin/bikunin precursor). This finding was validated by performing an ELISA experiment (p = 0.002). Conclusions and clinical relevance: The current study showed a clear difference between the protein profiles of CSF from patients with PE and normotensive pregnant women. Protein AMBP is a precursor of a heme-binding protein that counteracts the damaging effects of free hemoglobin, which may be related to the presence of free hemoglobin in CSF. Protein levels showed correlations with clinical symptoms during pregnancy and postpartum. To our knowledge, this is the first LC–MS/MS proteome profiling study on a unique set of CSF samples from (severe) preeclamptic patients and normotensive pregnant women.</p>},
  articleno    = {1600082},
  author       = {van den Berg, Caroline B. and Duvekot, Johannes J. and Güzel, Coşkun and Hansson, Stefan and de Leeuw, Thomas G. and Steegers, Eric A P and Versendaal, Johannes and Luider, Theo M. and Stoop, Marcel P.},
  issn         = {1862-8346},
  keyword      = {Blood–brain barrier,Cerebrospinal fluid,Preeclampsia,Pregnancy,Protein AMBP},
  language     = {eng},
  month        = {01},
  number       = {1-2},
  publisher    = {John Wiley & Sons},
  series       = {Proteomics - Clinical Applications},
  title        = {Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women},
  url          = {http://dx.doi.org/10.1002/prca.201600082},
  volume       = {11},
  year         = {2017},
}