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Cell immobilization on 3D-printed matrices : A model study on propionic acid fermentation

Belgrano, Fabricio dos Santos; Diegel, Olaf LU ; Pereira, Nei and Hatti-Kaul, Rajni LU (2018) In Bioresource Technology 249. p.777-782
Abstract

This study uses three-dimensional (3D) printing technology as a tool for designing carriers for immobilization of microbial cells for bioprocesses. Production of propionic acid from glucose by immobilized Propionibacterium sp. cells was studied as a model system. For cell adsorption, the 3D-printed nylon beads were added to the culture medium during 3 rounds of cell cultivation. Cell adsorption and fermentation kinetics were similar irrespective of the bead size and lattice structure. The cells bound to 15 mm beads exhibited reduced fermentation time as compared to free cell fermentations; maximum productivity and propionic acid titer of 0.46 g/L h and 25.8 g/L, respectively, were obtained. Treatment of the beads with polyethyleneimine... (More)

This study uses three-dimensional (3D) printing technology as a tool for designing carriers for immobilization of microbial cells for bioprocesses. Production of propionic acid from glucose by immobilized Propionibacterium sp. cells was studied as a model system. For cell adsorption, the 3D-printed nylon beads were added to the culture medium during 3 rounds of cell cultivation. Cell adsorption and fermentation kinetics were similar irrespective of the bead size and lattice structure. The cells bound to 15 mm beads exhibited reduced fermentation time as compared to free cell fermentations; maximum productivity and propionic acid titer of 0.46 g/L h and 25.8 g/L, respectively, were obtained. Treatment of the beads with polyethyleneimine improved cell-matrix binding, but lowered the productivity perhaps due to inhibitory effect of the polycation. Scanning electron micrographs revealed the cells to be located in crevices of the beads, but were more uniformly distributed on PEI-coated carrier indicating charge-charge interaction.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
3D-printed matrix, Cell immobilization, High cell density fermentation, Propionic acid fermentation
in
Bioresource Technology
volume
249
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:85032939256
ISSN
0960-8524
DOI
10.1016/j.biortech.2017.10.087
language
English
LU publication?
yes
id
b0bb6a59-7b56-435b-bec6-1777f43ef6f6
date added to LUP
2017-12-07 10:45:47
date last changed
2017-12-07 10:45:47
@article{b0bb6a59-7b56-435b-bec6-1777f43ef6f6,
  abstract     = {<p>This study uses three-dimensional (3D) printing technology as a tool for designing carriers for immobilization of microbial cells for bioprocesses. Production of propionic acid from glucose by immobilized Propionibacterium sp. cells was studied as a model system. For cell adsorption, the 3D-printed nylon beads were added to the culture medium during 3 rounds of cell cultivation. Cell adsorption and fermentation kinetics were similar irrespective of the bead size and lattice structure. The cells bound to 15 mm beads exhibited reduced fermentation time as compared to free cell fermentations; maximum productivity and propionic acid titer of 0.46 g/L h and 25.8 g/L, respectively, were obtained. Treatment of the beads with polyethyleneimine improved cell-matrix binding, but lowered the productivity perhaps due to inhibitory effect of the polycation. Scanning electron micrographs revealed the cells to be located in crevices of the beads, but were more uniformly distributed on PEI-coated carrier indicating charge-charge interaction.</p>},
  author       = {Belgrano, Fabricio dos Santos and Diegel, Olaf and Pereira, Nei and Hatti-Kaul, Rajni},
  issn         = {0960-8524},
  keyword      = {3D-printed matrix,Cell immobilization,High cell density fermentation,Propionic acid fermentation},
  language     = {eng},
  month        = {02},
  pages        = {777--782},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Cell immobilization on 3D-printed matrices : A model study on propionic acid fermentation},
  url          = {http://dx.doi.org/10.1016/j.biortech.2017.10.087},
  volume       = {249},
  year         = {2018},
}