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Circulating Levels of Brain-Enriched MicroRNAs Correlate with Neuron Specific Enolase after Cardiac Arrest-A Substudy of the Target Temperature Management Trial

Stefanizzi, Francesca Maria ; Nielsen, Niklas LU ; Zhang, Lu ; Dankiewicz, Josef LU ; Stammet, Pascal ; Gilje, Patrik LU ; Erlinge, David LU ; Hassager, Christian ; Wise, Matthew P. and Kuiper, Michael , et al. (2020) In International Journal of Molecular Sciences 21(12).
Abstract

Outcome prognostication after cardiac arrest (CA) is challenging. Current multimodal prediction approaches would benefit from new biomarkers. MicroRNAs constitute a novel class of disease markers and circulating levels of brain-enriched ones have been associated with outcome after CA. To determine whether these levels reflect the extent of brain damage in CA patients, we assessed their correlation with neuron-specific enolase (NSE), a marker of brain damage. Blood samples taken 48 h after return of spontaneous circulation from two groups of patients from the Targeted Temperature Management trial were used. Patients were grouped depending on their neurological outcome at six months. Circulating levels of microRNAs were assessed by... (More)

Outcome prognostication after cardiac arrest (CA) is challenging. Current multimodal prediction approaches would benefit from new biomarkers. MicroRNAs constitute a novel class of disease markers and circulating levels of brain-enriched ones have been associated with outcome after CA. To determine whether these levels reflect the extent of brain damage in CA patients, we assessed their correlation with neuron-specific enolase (NSE), a marker of brain damage. Blood samples taken 48 h after return of spontaneous circulation from two groups of patients from the Targeted Temperature Management trial were used. Patients were grouped depending on their neurological outcome at six months. Circulating levels of microRNAs were assessed by sequencing. NSE was measured at the same time-point. Among the 673 microRNAs detected, brain-enriched miR9-3p, miR124-3p and miR129-5p positively correlated with NSE levels (all p < 0.001). Interestingly, these correlations were absent when only the good outcome group was analyzed (p > 0.5). Moreover, these correlations were unaffected by demographic and clinical characteristics. All three microRNAs predicted neurological outcome at 6 months. Circulating levels of brain-enriched microRNAs are correlated with NSE levels and hence can reflect the extent of brain injury in patients after CA. This observation strengthens the potential of brain-enriched microRNAs to aid in outcome prognostication after CA.

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type
Contribution to journal
publication status
published
subject
keywords
biomarker, cardiac arrest, microRNAs, neurological function, prognostic
in
International Journal of Molecular Sciences
volume
21
issue
12
article number
4353
publisher
MDPI AG
external identifiers
  • pmid:32575355
  • scopus:85087002535
ISSN
1422-0067
DOI
10.3390/ijms21124353
language
English
LU publication?
yes
id
b0f79e1a-9e0a-454d-b9e2-60981327efc4
date added to LUP
2020-07-07 14:49:23
date last changed
2021-06-16 01:49:55
@article{b0f79e1a-9e0a-454d-b9e2-60981327efc4,
  abstract     = {<p>Outcome prognostication after cardiac arrest (CA) is challenging. Current multimodal prediction approaches would benefit from new biomarkers. MicroRNAs constitute a novel class of disease markers and circulating levels of brain-enriched ones have been associated with outcome after CA. To determine whether these levels reflect the extent of brain damage in CA patients, we assessed their correlation with neuron-specific enolase (NSE), a marker of brain damage. Blood samples taken 48 h after return of spontaneous circulation from two groups of patients from the Targeted Temperature Management trial were used. Patients were grouped depending on their neurological outcome at six months. Circulating levels of microRNAs were assessed by sequencing. NSE was measured at the same time-point. Among the 673 microRNAs detected, brain-enriched miR9-3p, miR124-3p and miR129-5p positively correlated with NSE levels (all p &lt; 0.001). Interestingly, these correlations were absent when only the good outcome group was analyzed (p &gt; 0.5). Moreover, these correlations were unaffected by demographic and clinical characteristics. All three microRNAs predicted neurological outcome at 6 months. Circulating levels of brain-enriched microRNAs are correlated with NSE levels and hence can reflect the extent of brain injury in patients after CA. This observation strengthens the potential of brain-enriched microRNAs to aid in outcome prognostication after CA.</p>},
  author       = {Stefanizzi, Francesca Maria and Nielsen, Niklas and Zhang, Lu and Dankiewicz, Josef and Stammet, Pascal and Gilje, Patrik and Erlinge, David and Hassager, Christian and Wise, Matthew P. and Kuiper, Michael and Friberg, Hans and Devaux, Yvan and Salgado-Somoza, Antonio},
  issn         = {1422-0067},
  language     = {eng},
  month        = {06},
  number       = {12},
  publisher    = {MDPI AG},
  series       = {International Journal of Molecular Sciences},
  title        = {Circulating Levels of Brain-Enriched MicroRNAs Correlate with Neuron Specific Enolase after Cardiac Arrest-A Substudy of the Target Temperature Management Trial},
  url          = {http://dx.doi.org/10.3390/ijms21124353},
  doi          = {10.3390/ijms21124353},
  volume       = {21},
  year         = {2020},
}