DNA concentration wave formation in pillar arrays
(2020) 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences p.240-241- Abstract
High throughput in particle and molecular sorting schemes is an important performance indicator and can be realized through increased volumetric processing rates or increased concentrations. Here we investigate the effect of increased concentration of high-molecular weight DNA in micropillar arrays for deterministic lateral displacement (DLD). We find that the array imposes regular fluctuations in the concentration of the DNA if the sample concentration and flow rates exceed respective threshold values. We characterize the resulting concentration waves and study their influence on microsphere trajectories in the device. We expect our results to be relevant e.g. for sample preparation of cell lysates which can often be complicated by the... (More)
High throughput in particle and molecular sorting schemes is an important performance indicator and can be realized through increased volumetric processing rates or increased concentrations. Here we investigate the effect of increased concentration of high-molecular weight DNA in micropillar arrays for deterministic lateral displacement (DLD). We find that the array imposes regular fluctuations in the concentration of the DNA if the sample concentration and flow rates exceed respective threshold values. We characterize the resulting concentration waves and study their influence on microsphere trajectories in the device. We expect our results to be relevant e.g. for sample preparation of cell lysates which can often be complicated by the release of chromosomal DNA.
(Less)
- author
- Ström, Oskar E.
LU
; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU
- organization
- publishing date
- 2020
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- DNA concentration waves, DNA entanglement, Pillar Arrays
- host publication
- MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- series title
- MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
- conference location
- Virtual, Online
- conference dates
- 2020-10-04 - 2020-10-09
- external identifiers
-
- scopus:85098244160
- ISBN
- 9781733419017
- language
- English
- LU publication?
- yes
- id
- b138de33-9722-428a-92de-5c5f5c9715a8
- date added to LUP
- 2021-01-11 07:35:43
- date last changed
- 2023-11-20 19:39:27
@inproceedings{b138de33-9722-428a-92de-5c5f5c9715a8, abstract = {{<p>High throughput in particle and molecular sorting schemes is an important performance indicator and can be realized through increased volumetric processing rates or increased concentrations. Here we investigate the effect of increased concentration of high-molecular weight DNA in micropillar arrays for deterministic lateral displacement (DLD). We find that the array imposes regular fluctuations in the concentration of the DNA if the sample concentration and flow rates exceed respective threshold values. We characterize the resulting concentration waves and study their influence on microsphere trajectories in the device. We expect our results to be relevant e.g. for sample preparation of cell lysates which can often be complicated by the release of chromosomal DNA.</p>}}, author = {{Ström, Oskar E. and Beech, Jason P. and Tegenfeldt, Jonas O.}}, booktitle = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, isbn = {{9781733419017}}, keywords = {{DNA concentration waves; DNA entanglement; Pillar Arrays}}, language = {{eng}}, pages = {{240--241}}, publisher = {{Chemical and Biological Microsystems Society}}, series = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, title = {{DNA concentration wave formation in pillar arrays}}, year = {{2020}}, }