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DNA concentration wave formation in pillar arrays

Ström, Oskar E. LU orcid ; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU orcid (2020) 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences p.240-241
Abstract

High throughput in particle and molecular sorting schemes is an important performance indicator and can be realized through increased volumetric processing rates or increased concentrations. Here we investigate the effect of increased concentration of high-molecular weight DNA in micropillar arrays for deterministic lateral displacement (DLD). We find that the array imposes regular fluctuations in the concentration of the DNA if the sample concentration and flow rates exceed respective threshold values. We characterize the resulting concentration waves and study their influence on microsphere trajectories in the device. We expect our results to be relevant e.g. for sample preparation of cell lysates which can often be complicated by the... (More)

High throughput in particle and molecular sorting schemes is an important performance indicator and can be realized through increased volumetric processing rates or increased concentrations. Here we investigate the effect of increased concentration of high-molecular weight DNA in micropillar arrays for deterministic lateral displacement (DLD). We find that the array imposes regular fluctuations in the concentration of the DNA if the sample concentration and flow rates exceed respective threshold values. We characterize the resulting concentration waves and study their influence on microsphere trajectories in the device. We expect our results to be relevant e.g. for sample preparation of cell lysates which can often be complicated by the release of chromosomal DNA.

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Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
DNA concentration waves, DNA entanglement, Pillar Arrays
host publication
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
series title
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
conference location
Virtual, Online
conference dates
2020-10-04 - 2020-10-09
external identifiers
  • scopus:85098244160
ISBN
9781733419017
language
English
LU publication?
yes
id
b138de33-9722-428a-92de-5c5f5c9715a8
date added to LUP
2021-01-11 07:35:43
date last changed
2023-11-20 19:39:27
@inproceedings{b138de33-9722-428a-92de-5c5f5c9715a8,
  abstract     = {{<p>High throughput in particle and molecular sorting schemes is an important performance indicator and can be realized through increased volumetric processing rates or increased concentrations. Here we investigate the effect of increased concentration of high-molecular weight DNA in micropillar arrays for deterministic lateral displacement (DLD). We find that the array imposes regular fluctuations in the concentration of the DNA if the sample concentration and flow rates exceed respective threshold values. We characterize the resulting concentration waves and study their influence on microsphere trajectories in the device. We expect our results to be relevant e.g. for sample preparation of cell lysates which can often be complicated by the release of chromosomal DNA.</p>}},
  author       = {{Ström, Oskar E. and Beech, Jason P. and Tegenfeldt, Jonas O.}},
  booktitle    = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  isbn         = {{9781733419017}},
  keywords     = {{DNA concentration waves; DNA entanglement; Pillar Arrays}},
  language     = {{eng}},
  pages        = {{240--241}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  series       = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  title        = {{DNA concentration wave formation in pillar arrays}},
  year         = {{2020}},
}