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Ultra-high performance supercritical fluid chromatography of lignin-derived phenols from alkaline cupric oxide oxidation

Sun, Mingzhe LU ; Lidén, Gunnar LU ; Sandahl, Margareta LU and Turner, Charlotta LU (2016) In Journal of Separation Science 39(16). p.3123-3129
Abstract

Traditional chromatographic methods for the analysis of lignin-derived phenolic compounds in environmental samples are generally time consuming. In this work, an ultra-high performance supercritical fluid chromatography method with a diode array detector for the analysis of major lignin-derived phenolic compounds produced by alkaline cupric oxide oxidation was developed. In an analysis of a collection of 11 representative monomeric lignin phenolic compounds, all compounds were clearly separated within 6 min with excellent peak shapes, with a limit of detection of 0.5–2.5 μM, a limit of quantification of 2.5–5.0 μM, and a dynamic range of 5.0–2.0 mM (R2 > 0.997). The new ultra-high performance supercritical fluid... (More)

Traditional chromatographic methods for the analysis of lignin-derived phenolic compounds in environmental samples are generally time consuming. In this work, an ultra-high performance supercritical fluid chromatography method with a diode array detector for the analysis of major lignin-derived phenolic compounds produced by alkaline cupric oxide oxidation was developed. In an analysis of a collection of 11 representative monomeric lignin phenolic compounds, all compounds were clearly separated within 6 min with excellent peak shapes, with a limit of detection of 0.5–2.5 μM, a limit of quantification of 2.5–5.0 μM, and a dynamic range of 5.0–2.0 mM (R2 > 0.997). The new ultra-high performance supercritical fluid chromatography method was also applied for the qualitative and quantitative analysis of lignin-derived phenolic compounds obtained upon alkaline cupric oxide oxidation of a commercial humic acid. Ten out of the previous eleven model compounds could be quantified in the oxidized humic acid sample. The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CuO oxidation, Humic acid, Lignin, Phenolic compounds, Ultra-high performance supercritical fluid chromatography
in
Journal of Separation Science
volume
39
issue
16
pages
7 pages
publisher
John Wiley & Sons
external identifiers
  • scopus:84983375212
  • wos:000383361800004
ISSN
1615-9306
DOI
10.1002/jssc.201600169
language
English
LU publication?
yes
id
b1fe9f28-b8de-4324-882c-eee0f63afb61
date added to LUP
2016-12-09 14:19:31
date last changed
2017-05-28 04:55:32
@article{b1fe9f28-b8de-4324-882c-eee0f63afb61,
  abstract     = {<p>Traditional chromatographic methods for the analysis of lignin-derived phenolic compounds in environmental samples are generally time consuming. In this work, an ultra-high performance supercritical fluid chromatography method with a diode array detector for the analysis of major lignin-derived phenolic compounds produced by alkaline cupric oxide oxidation was developed. In an analysis of a collection of 11 representative monomeric lignin phenolic compounds, all compounds were clearly separated within 6 min with excellent peak shapes, with a limit of detection of 0.5–2.5 μM, a limit of quantification of 2.5–5.0 μM, and a dynamic range of 5.0–2.0 mM (R<sup>2</sup> &gt; 0.997). The new ultra-high performance supercritical fluid chromatography method was also applied for the qualitative and quantitative analysis of lignin-derived phenolic compounds obtained upon alkaline cupric oxide oxidation of a commercial humic acid. Ten out of the previous eleven model compounds could be quantified in the oxidized humic acid sample. The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples.</p>},
  author       = {Sun, Mingzhe and Lidén, Gunnar and Sandahl, Margareta and Turner, Charlotta},
  issn         = {1615-9306},
  keyword      = {CuO oxidation,Humic acid,Lignin,Phenolic compounds,Ultra-high performance supercritical fluid chromatography},
  language     = {eng},
  month        = {08},
  number       = {16},
  pages        = {3123--3129},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Separation Science},
  title        = {Ultra-high performance supercritical fluid chromatography of lignin-derived phenols from alkaline cupric oxide oxidation},
  url          = {http://dx.doi.org/10.1002/jssc.201600169},
  volume       = {39},
  year         = {2016},
}