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Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure

Törnquist, Elin LU ; Gentile, Luigi LU ; Prevost, Sylvain ; Diaz, Ana ; Olsson, Ulf LU and Isaksson, Hanna LU (2020) In Scientific Reports 10(1).
Abstract
In this study, we present a combined small-angle neutron and X-ray scattering (SANS and SAXS) study of the nanoscale structure of cortical bone specimens from three different species. The variation of the scattering cross section of elements across the periodic table is very different for neutrons and X-rays. For X-rays, it is proportional to the electron density while for neutrons it varies irregularly with the atomic number. Hence, combining the two techniques on the same specimens allows for a more detailed interpretation of the scattering patterns as compared to a single-contrast experiment. The current study was performed on bovine, porcine and ovine specimens, obtained in two perpendicular directions with respect to the main axis of... (More)
In this study, we present a combined small-angle neutron and X-ray scattering (SANS and SAXS) study of the nanoscale structure of cortical bone specimens from three different species. The variation of the scattering cross section of elements across the periodic table is very different for neutrons and X-rays. For X-rays, it is proportional to the electron density while for neutrons it varies irregularly with the atomic number. Hence, combining the two techniques on the same specimens allows for a more detailed interpretation of the scattering patterns as compared to a single-contrast experiment. The current study was performed on bovine, porcine and ovine specimens, obtained in two perpendicular directions with respect to the main axis of the bone (longitudinal and radial) in order to maximise the understanding of the nanostructural organisation. The specimens were also imaged with high resolution micro-computed tomography (micro-CT), yielding tissue mineral density and microstructural orientation as reference. We show that the SANS and SAXS patterns from the same specimen are effectively identical, suggesting that these bone specimens can be approximated as a two-component composite material. Hence, the observed small-angle scattering results mainly from the mineral-collagen contrast, apart from minor features associated with the internal collagen structure. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
10
issue
1
article number
14552
pages
11 pages
publisher
Nature Publishing Group
external identifiers
  • pmid:32883964
  • scopus:85090115551
ISSN
2045-2322
DOI
10.1038/s41598-020-71190-9
language
English
LU publication?
yes
id
b2062678-ad97-423c-9b2e-dea163903a86
date added to LUP
2020-09-04 08:08:36
date last changed
2020-09-29 12:06:51
@article{b2062678-ad97-423c-9b2e-dea163903a86,
  abstract     = {In this study, we present a combined small-angle neutron and X-ray scattering (SANS and SAXS) study of the nanoscale structure of cortical bone specimens from three different species. The variation of the scattering cross section of elements across the periodic table is very different for neutrons and X-rays. For X-rays, it is proportional to the electron density while for neutrons it varies irregularly with the atomic number. Hence, combining the two techniques on the same specimens allows for a more detailed interpretation of the scattering patterns as compared to a single-contrast experiment. The current study was performed on bovine, porcine and ovine specimens, obtained in two perpendicular directions with respect to the main axis of the bone (longitudinal and radial) in order to maximise the understanding of the nanostructural organisation. The specimens were also imaged with high resolution micro-computed tomography (micro-CT), yielding tissue mineral density and microstructural orientation as reference. We show that the SANS and SAXS patterns from the same specimen are effectively identical, suggesting that these bone specimens can be approximated as a two-component composite material. Hence, the observed small-angle scattering results mainly from the mineral-collagen contrast, apart from minor features associated with the internal collagen structure.},
  author       = {Törnquist, Elin and Gentile, Luigi and Prevost, Sylvain and Diaz, Ana and Olsson, Ulf and Isaksson, Hanna},
  issn         = {2045-2322},
  language     = {eng},
  month        = {09},
  number       = {1},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure},
  url          = {http://dx.doi.org/10.1038/s41598-020-71190-9},
  doi          = {10.1038/s41598-020-71190-9},
  volume       = {10},
  year         = {2020},
}