DNA fragmentation characteristic of apoptosis and cell loss induced by kainic acid in rabbit retinas
Perez, M T LU ; Arnér, K LU and Håkansson, Anders P LU
(1997)
In Neurochemistry International
31(2).
p.60-251
- Abstract
We have examined whether in vivo exposure to the glutamate analogue, kainic acid, induces cell loss through apoptosis and/or through necrosis. The vulnerability of rabbit retinal cells was evaluated by routine histopathology. The DNA fragmentation was examined using an in situ method (TUNEL: TdT-mediated biotin-dUTP nick-end labelling) and agarose gel electrophoresis of extracted retinal DNA. Retinas were examined at 30 min, and 4, 16, 24 and 36 h, and 2-5 days following the intraocular administration of 140 nmol kainic acid. Although pyknotic cells could be seen already at 30 min post-injection, TUNEL-labelled nuclei were first observed 4 h after the injection. A relatively large number of pyknotic cells and of TUNEL-labelled nuclei... (More)
We have examined whether in vivo exposure to the glutamate analogue, kainic acid, induces cell loss through apoptosis and/or through necrosis. The vulnerability of rabbit retinal cells was evaluated by routine histopathology. The DNA fragmentation was examined using an in situ method (TUNEL: TdT-mediated biotin-dUTP nick-end labelling) and agarose gel electrophoresis of extracted retinal DNA. Retinas were examined at 30 min, and 4, 16, 24 and 36 h, and 2-5 days following the intraocular administration of 140 nmol kainic acid. Although pyknotic cells could be seen already at 30 min post-injection, TUNEL-labelled nuclei were first observed 4 h after the injection. A relatively large number of pyknotic cells and of TUNEL-labelled nuclei were still seen at 5 days post-injection. Pyknotic cells were seen throughout the inner nuclear layer (mostly in the proximal half of the layer) and in the ganglion cell layer. The TUNEL-labelled nuclei were almost only seen in the proximal inner nuclear layer. Analysis of DNA by electrophoresis revealed the presence of large molecular weight fragments 4 h after the injection, and of oligonucleosome-size fragments between 16 h and 2 days after the injection. The present study thus presents evidence that, in our model, the retinal cell loss induced by kainic acid is preceded, probably in most cells, by a fragmentation of DNA characteristic of apoptotic cell death. The process of cell loss following kainic acid administration was found to be relatively slow, further suggesting that a programmed type of cell death, which eventually induces apoptosis, is involved. No indication that cells were lost also through necrosis was obtained.
(Less)
- author
- Perez, M T
LU
; Arnér, K
LU
and Håkansson, Anders P
LU
- organization
- publishing date
- 1997-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Apoptosis, DNA Fragmentation, Electrophoresis, Agar Gel, Female, Genetic Techniques, Kainic Acid, Male, Rabbits, Retina
- in
- Neurochemistry International
- volume
- 31
- issue
- 2
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:9220458
- scopus:0030971574
- ISSN
- 0197-0186
- language
- English
- LU publication?
- yes
- id
- b2a37a66-8186-4817-bfe8-5de4082194d2
- date added to LUP
- 2016-05-21 13:58:05
- date last changed
- 2024-01-04 04:18:24
@article{b2a37a66-8186-4817-bfe8-5de4082194d2,
abstract = {{<p>We have examined whether in vivo exposure to the glutamate analogue, kainic acid, induces cell loss through apoptosis and/or through necrosis. The vulnerability of rabbit retinal cells was evaluated by routine histopathology. The DNA fragmentation was examined using an in situ method (TUNEL: TdT-mediated biotin-dUTP nick-end labelling) and agarose gel electrophoresis of extracted retinal DNA. Retinas were examined at 30 min, and 4, 16, 24 and 36 h, and 2-5 days following the intraocular administration of 140 nmol kainic acid. Although pyknotic cells could be seen already at 30 min post-injection, TUNEL-labelled nuclei were first observed 4 h after the injection. A relatively large number of pyknotic cells and of TUNEL-labelled nuclei were still seen at 5 days post-injection. Pyknotic cells were seen throughout the inner nuclear layer (mostly in the proximal half of the layer) and in the ganglion cell layer. The TUNEL-labelled nuclei were almost only seen in the proximal inner nuclear layer. Analysis of DNA by electrophoresis revealed the presence of large molecular weight fragments 4 h after the injection, and of oligonucleosome-size fragments between 16 h and 2 days after the injection. The present study thus presents evidence that, in our model, the retinal cell loss induced by kainic acid is preceded, probably in most cells, by a fragmentation of DNA characteristic of apoptotic cell death. The process of cell loss following kainic acid administration was found to be relatively slow, further suggesting that a programmed type of cell death, which eventually induces apoptosis, is involved. No indication that cells were lost also through necrosis was obtained.</p>}},
author = {{Perez, M T and Arnér, K and Håkansson, Anders P}},
issn = {{0197-0186}},
keywords = {{Animals; Apoptosis; DNA Fragmentation; Electrophoresis, Agar Gel; Female; Genetic Techniques; Kainic Acid; Male; Rabbits; Retina}},
language = {{eng}},
number = {{2}},
pages = {{60--251}},
publisher = {{Elsevier}},
series = {{Neurochemistry International}},
title = {{DNA fragmentation characteristic of apoptosis and cell loss induced by kainic acid in rabbit retinas}},
volume = {{31}},
year = {{1997}},
}