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Rapid and direct determination of fructose in food: A new osmium-polymer mediated biosensor

Antiochia, Riccarda; Vinci, Giuliana and Gorton, Lo LU (2013) In Food Chemistry 140(4). p.742-747
Abstract
This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of I mu M, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 mu A... (More)
This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of I mu M, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 mu A cm(-2) mM), good reproducibility (RSD = 2.1%) and a fast response time (4 s). Finally, the biosensor was applied for specific determination of fructose in honey, fruit juices, soft and energy drinks. The results indicated a very good agreement with those obtained with a commercial reference kit. No significant interference was observed with the proposed biosensor. (C) 2012 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fructose, Food analysis, Biosensor, Fructose dehydrogenase, Osmium redox, polymer
in
Food Chemistry
volume
140
issue
4
pages
742 - 747
publisher
Elsevier
external identifiers
  • wos:000321083800019
  • scopus:84878017647
ISSN
1873-7072
DOI
10.1016/j.foodchem.2012.11.023
language
English
LU publication?
yes
id
b34614b2-9af0-4c37-891e-fc8dc0919455 (old id 3975344)
date added to LUP
2013-08-21 16:59:59
date last changed
2019-07-09 02:36:17
@article{b34614b2-9af0-4c37-891e-fc8dc0919455,
  abstract     = {This paper describes the development and performance of a new rapid amperometric biosensor for fructose monitoring in food analysis. The biosensor is based on the activity of fructose dehydrogenase (FDH) immobilised into a carbon nanotube paste electrode according to two different procedures. The direct wiring of the FDH in a highly original osmium-polymer hydrogel was found to offer a better enzyme entrapment compared to the immobilisation of the enzyme in an albumin hydrogel. The optimised biosensor required only 5 U of FDH and kept the 80% of its initial sensitivity after 4 months. During this time, the biosensor showed a detection limit for fructose of I mu M, a large linear range between 0.1 and 5 mM, a high sensitivity (1.95 mu A cm(-2) mM), good reproducibility (RSD = 2.1%) and a fast response time (4 s). Finally, the biosensor was applied for specific determination of fructose in honey, fruit juices, soft and energy drinks. The results indicated a very good agreement with those obtained with a commercial reference kit. No significant interference was observed with the proposed biosensor. (C) 2012 Elsevier Ltd. All rights reserved.},
  author       = {Antiochia, Riccarda and Vinci, Giuliana and Gorton, Lo},
  issn         = {1873-7072},
  keyword      = {Fructose,Food analysis,Biosensor,Fructose dehydrogenase,Osmium redox,polymer},
  language     = {eng},
  number       = {4},
  pages        = {742--747},
  publisher    = {Elsevier},
  series       = {Food Chemistry},
  title        = {Rapid and direct determination of fructose in food: A new osmium-polymer mediated biosensor},
  url          = {http://dx.doi.org/10.1016/j.foodchem.2012.11.023},
  volume       = {140},
  year         = {2013},
}