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Effect of recombinant neutral endopeptidase (EC 3.4.24.11) on neuropeptide-mediated nasal fluid secretion and plasma exudation in the rat

Josefsson, Malin LU ; Becker, Magnus LU orcid ; Stroman, F ; Brenner, Daniel G and Petersson, Göran (1998) In American Journal of Rhinology 12(2). p.41-135
Abstract

The nasal mucosa harbors sensory nerves containing neuropeptides such as substance P (SP), which are released by capsaicin. The neuropeptides are degraded by peptidases, e.g., neutral endopeptidase (NEP) that is present in the nasal mucosa. We studied the effect of enzymatically active recombinant NEP (rNEP) on neuropeptide-evoked secretion of nasal fluid and plasma exudation in rats. rNEP administered intranasally (i.n.) reduced the capsaicin-evoked nasal fluid secretion but failed to reduce the secretion evoked by SP (exogenous) under the experimental conditions used. rNEP reduced the increase in nasal plasma exudation evoked by capsaicin (endogenous neuropeptides). Because rNEP reduced neuropeptide-mediated nasal fluid secretion and... (More)

The nasal mucosa harbors sensory nerves containing neuropeptides such as substance P (SP), which are released by capsaicin. The neuropeptides are degraded by peptidases, e.g., neutral endopeptidase (NEP) that is present in the nasal mucosa. We studied the effect of enzymatically active recombinant NEP (rNEP) on neuropeptide-evoked secretion of nasal fluid and plasma exudation in rats. rNEP administered intranasally (i.n.) reduced the capsaicin-evoked nasal fluid secretion but failed to reduce the secretion evoked by SP (exogenous) under the experimental conditions used. rNEP reduced the increase in nasal plasma exudation evoked by capsaicin (endogenous neuropeptides). Because rNEP reduced neuropeptide-mediated nasal fluid secretion and plasma exudation in the rat, we suggest that peptidase activity in the nasal mucosa will determine the magnitude of the response to locally released neuropeptides.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Administration, Topical, Animals, Capsaicin/pharmacology, Exudates and Transudates/metabolism, Male, Nasal Mucosa/drug effects, Neprilysin/pharmacology, Neuropeptides/physiology, Plasma/metabolism, Rats, Rats, Sprague-Dawley, Recombinant Proteins, Substance P/pharmacology
in
American Journal of Rhinology
volume
12
issue
2
pages
7 pages
publisher
OceanSide Publications, Inc
external identifiers
  • pmid:9578933
  • scopus:0031981760
ISSN
1050-6586
DOI
10.2500/105065898781390244
language
English
LU publication?
no
id
b35e3878-1086-4993-9612-1dab555e5649
date added to LUP
2021-04-19 16:37:56
date last changed
2024-01-05 09:33:48
@article{b35e3878-1086-4993-9612-1dab555e5649,
  abstract     = {{<p>The nasal mucosa harbors sensory nerves containing neuropeptides such as substance P (SP), which are released by capsaicin. The neuropeptides are degraded by peptidases, e.g., neutral endopeptidase (NEP) that is present in the nasal mucosa. We studied the effect of enzymatically active recombinant NEP (rNEP) on neuropeptide-evoked secretion of nasal fluid and plasma exudation in rats. rNEP administered intranasally (i.n.) reduced the capsaicin-evoked nasal fluid secretion but failed to reduce the secretion evoked by SP (exogenous) under the experimental conditions used. rNEP reduced the increase in nasal plasma exudation evoked by capsaicin (endogenous neuropeptides). Because rNEP reduced neuropeptide-mediated nasal fluid secretion and plasma exudation in the rat, we suggest that peptidase activity in the nasal mucosa will determine the magnitude of the response to locally released neuropeptides.</p>}},
  author       = {{Josefsson, Malin and Becker, Magnus and Stroman, F and Brenner, Daniel  G and Petersson, Göran}},
  issn         = {{1050-6586}},
  keywords     = {{Administration, Topical; Animals; Capsaicin/pharmacology; Exudates and Transudates/metabolism; Male; Nasal Mucosa/drug effects; Neprilysin/pharmacology; Neuropeptides/physiology; Plasma/metabolism; Rats; Rats, Sprague-Dawley; Recombinant Proteins; Substance P/pharmacology}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{2}},
  pages        = {{41--135}},
  publisher    = {{OceanSide Publications, Inc}},
  series       = {{American Journal of Rhinology}},
  title        = {{Effect of recombinant neutral endopeptidase (EC 3.4.24.11) on neuropeptide-mediated nasal fluid secretion and plasma exudation in the rat}},
  url          = {{http://dx.doi.org/10.2500/105065898781390244}},
  doi          = {{10.2500/105065898781390244}},
  volume       = {{12}},
  year         = {{1998}},
}