Muconic Acid Production Using Engineered Pseudomonas putida KT2440 and a Guaiacol-Rich Fraction Derived from Kraft Lignin
(2021) In ACS Sustainable Chemistry and Engineering 9(24). p.8097-8106- Abstract
Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and materials. In this study, a process was developed for depolymerization of kraft lignin followed by an upgrading separation step and further bioconversion of the obtained monoaromatic compounds to muconic acid. First, industrial kraft lignin, Indulin AT, was processed into a guaiacol-rich stream using base-catalyzed depolymerization. This stream was subsequently upgraded using liquid-liquid extraction and evaporation to yield a more concentrated and less inhibitory stream, adapted for bioconversion. Finally, guaiacol was quantitatively converted to muconic acid through bioconversion using an engineered Pseudomonas putida strain containing... (More)
Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and materials. In this study, a process was developed for depolymerization of kraft lignin followed by an upgrading separation step and further bioconversion of the obtained monoaromatic compounds to muconic acid. First, industrial kraft lignin, Indulin AT, was processed into a guaiacol-rich stream using base-catalyzed depolymerization. This stream was subsequently upgraded using liquid-liquid extraction and evaporation to yield a more concentrated and less inhibitory stream, adapted for bioconversion. Finally, guaiacol was quantitatively converted to muconic acid through bioconversion using an engineered Pseudomonas putida strain containing cytochrome P450 and ferredoxin reductase for guaiacol assimilation and deletion of the native catBC genes for muconic acid production. Isomerization of muconic acid in a fermentation medium depending on pH was also studied.
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- author
- Almqvist, Henrik LU ; Veras, Henrique ; Li, Kena LU ; Garcia Hidalgo, Javier LU ; Hulteberg, Christian LU ; Gorwa-Grauslund, Marie LU ; Skorupa Parachin, Nádia LU and Carlquist, Magnus LU
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- bioconversion, guaiacol, lignin, muconic acid, Pseudomonas putida
- in
- ACS Sustainable Chemistry and Engineering
- volume
- 9
- issue
- 24
- pages
- 8097 - 8106
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- scopus:85108668343
- ISSN
- 2168-0485
- DOI
- 10.1021/acssuschemeng.1c00933
- project
- STEPS – Sustainable Plastics and Transition Pathways, Phase 2
- language
- English
- LU publication?
- yes
- id
- b41b9c90-6516-44cd-a884-cd15028deafe
- date added to LUP
- 2021-07-20 16:03:12
- date last changed
- 2023-12-21 23:11:54
@article{b41b9c90-6516-44cd-a884-cd15028deafe, abstract = {{<p>Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and materials. In this study, a process was developed for depolymerization of kraft lignin followed by an upgrading separation step and further bioconversion of the obtained monoaromatic compounds to muconic acid. First, industrial kraft lignin, Indulin AT, was processed into a guaiacol-rich stream using base-catalyzed depolymerization. This stream was subsequently upgraded using liquid-liquid extraction and evaporation to yield a more concentrated and less inhibitory stream, adapted for bioconversion. Finally, guaiacol was quantitatively converted to muconic acid through bioconversion using an engineered <i>Pseudomonas putida</i> strain containing cytochrome P450 and ferredoxin reductase for guaiacol assimilation and deletion of the native <i>cat</i>BC genes for muconic acid production. Isomerization of muconic acid in a fermentation medium depending on pH was also studied. </p>}}, author = {{Almqvist, Henrik and Veras, Henrique and Li, Kena and Garcia Hidalgo, Javier and Hulteberg, Christian and Gorwa-Grauslund, Marie and Skorupa Parachin, Nádia and Carlquist, Magnus}}, issn = {{2168-0485}}, keywords = {{bioconversion; guaiacol; lignin; muconic acid; Pseudomonas putida}}, language = {{eng}}, number = {{24}}, pages = {{8097--8106}}, publisher = {{The American Chemical Society (ACS)}}, series = {{ACS Sustainable Chemistry and Engineering}}, title = {{Muconic Acid Production Using Engineered <i>Pseudomonas putida</i> KT2440 and a Guaiacol-Rich Fraction Derived from Kraft Lignin}}, url = {{http://dx.doi.org/10.1021/acssuschemeng.1c00933}}, doi = {{10.1021/acssuschemeng.1c00933}}, volume = {{9}}, year = {{2021}}, }