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Muconic Acid Production Using Engineered Pseudomonas putida KT2440 and a Guaiacol-Rich Fraction Derived from Kraft Lignin

Almqvist, Henrik LU ; Veras, Henrique ; Li, Kena LU orcid ; Garcia Hidalgo, Javier LU ; Hulteberg, Christian LU orcid ; Gorwa-Grauslund, Marie LU ; Skorupa Parachin, Nádia LU and Carlquist, Magnus LU (2021) In ACS Sustainable Chemistry and Engineering 9(24). p.8097-8106
Abstract

Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and materials. In this study, a process was developed for depolymerization of kraft lignin followed by an upgrading separation step and further bioconversion of the obtained monoaromatic compounds to muconic acid. First, industrial kraft lignin, Indulin AT, was processed into a guaiacol-rich stream using base-catalyzed depolymerization. This stream was subsequently upgraded using liquid-liquid extraction and evaporation to yield a more concentrated and less inhibitory stream, adapted for bioconversion. Finally, guaiacol was quantitatively converted to muconic acid through bioconversion using an engineered Pseudomonas putida strain containing... (More)

Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and materials. In this study, a process was developed for depolymerization of kraft lignin followed by an upgrading separation step and further bioconversion of the obtained monoaromatic compounds to muconic acid. First, industrial kraft lignin, Indulin AT, was processed into a guaiacol-rich stream using base-catalyzed depolymerization. This stream was subsequently upgraded using liquid-liquid extraction and evaporation to yield a more concentrated and less inhibitory stream, adapted for bioconversion. Finally, guaiacol was quantitatively converted to muconic acid through bioconversion using an engineered Pseudomonas putida strain containing cytochrome P450 and ferredoxin reductase for guaiacol assimilation and deletion of the native catBC genes for muconic acid production. Isomerization of muconic acid in a fermentation medium depending on pH was also studied.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bioconversion, guaiacol, lignin, muconic acid, Pseudomonas putida
in
ACS Sustainable Chemistry and Engineering
volume
9
issue
24
pages
8097 - 8106
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85108668343
ISSN
2168-0485
DOI
10.1021/acssuschemeng.1c00933
project
STEPS – Sustainable Plastics and Transition Pathways, Phase 2
language
English
LU publication?
yes
id
b41b9c90-6516-44cd-a884-cd15028deafe
date added to LUP
2021-07-20 16:03:12
date last changed
2023-12-21 23:11:54
@article{b41b9c90-6516-44cd-a884-cd15028deafe,
  abstract     = {{<p>Industrial lignin such as kraft lignin is an abundant feedstock for renewable chemicals and materials. In this study, a process was developed for depolymerization of kraft lignin followed by an upgrading separation step and further bioconversion of the obtained monoaromatic compounds to muconic acid. First, industrial kraft lignin, Indulin AT, was processed into a guaiacol-rich stream using base-catalyzed depolymerization. This stream was subsequently upgraded using liquid-liquid extraction and evaporation to yield a more concentrated and less inhibitory stream, adapted for bioconversion. Finally, guaiacol was quantitatively converted to muconic acid through bioconversion using an engineered <i>Pseudomonas putida</i> strain containing cytochrome P450 and ferredoxin reductase for guaiacol assimilation and deletion of the native <i>cat</i>BC genes for muconic acid production. Isomerization of muconic acid in a fermentation medium depending on pH was also studied. </p>}},
  author       = {{Almqvist, Henrik and Veras, Henrique and Li, Kena and Garcia Hidalgo, Javier and Hulteberg, Christian and Gorwa-Grauslund, Marie and Skorupa Parachin, Nádia and Carlquist, Magnus}},
  issn         = {{2168-0485}},
  keywords     = {{bioconversion; guaiacol; lignin; muconic acid; Pseudomonas putida}},
  language     = {{eng}},
  number       = {{24}},
  pages        = {{8097--8106}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Sustainable Chemistry and Engineering}},
  title        = {{Muconic Acid Production Using Engineered <i>Pseudomonas putida</i> KT2440 and a Guaiacol-Rich Fraction Derived from Kraft Lignin}},
  url          = {{http://dx.doi.org/10.1021/acssuschemeng.1c00933}},
  doi          = {{10.1021/acssuschemeng.1c00933}},
  volume       = {{9}},
  year         = {{2021}},
}