A sol-gel integrated dual-readout microarray platform for quantification and identification of prostate-specific antigen
(2018) In Analytical Sciences 34(3). p.317-321- Abstract
Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z =... (More)
Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z = 1272, 1407, and 1872). The sol-gel microarray platform enables dual readout of PSA both fluorometric and MALDI-TOF MS analysis in biological samples. Here we report a useful method for a means for discovery of biomarkers in complex body fluids.
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- author
- Lee, Sang Wook LU ; Lee, Jong Hyun ; Kwon, Hyuck Gi ; Laurell, Thomas LU ; Jeong, Ok Chan and Kim, Soyoun
- organization
- publishing date
- 2018
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biomarker discovery, Macro-structure silicon, MALDI-TOF mass spectrometry, PSA, Sensitive diagnosis, Sol-gel microarray
- in
- Analytical Sciences
- volume
- 34
- issue
- 3
- pages
- 5 pages
- publisher
- Japan Society for Analytical Chemistry
- external identifiers
-
- pmid:29526899
- scopus:85043372493
- ISSN
- 0910-6340
- DOI
- 10.2116/analsci.34.317
- language
- English
- LU publication?
- yes
- id
- b4307f54-d99c-43fc-b91f-af51131c294a
- date added to LUP
- 2018-03-22 15:07:37
- date last changed
- 2024-01-29 13:28:42
@article{b4307f54-d99c-43fc-b91f-af51131c294a, abstract = {{<p>Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z = 1272, 1407, and 1872). The sol-gel microarray platform enables dual readout of PSA both fluorometric and MALDI-TOF MS analysis in biological samples. Here we report a useful method for a means for discovery of biomarkers in complex body fluids.</p>}}, author = {{Lee, Sang Wook and Lee, Jong Hyun and Kwon, Hyuck Gi and Laurell, Thomas and Jeong, Ok Chan and Kim, Soyoun}}, issn = {{0910-6340}}, keywords = {{Biomarker discovery; Macro-structure silicon; MALDI-TOF mass spectrometry; PSA; Sensitive diagnosis; Sol-gel microarray}}, language = {{eng}}, number = {{3}}, pages = {{317--321}}, publisher = {{Japan Society for Analytical Chemistry}}, series = {{Analytical Sciences}}, title = {{A sol-gel integrated dual-readout microarray platform for quantification and identification of prostate-specific antigen}}, url = {{http://dx.doi.org/10.2116/analsci.34.317}}, doi = {{10.2116/analsci.34.317}}, volume = {{34}}, year = {{2018}}, }