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A sol-gel integrated dual-readout microarray platform for quantification and identification of prostate-specific antigen

Lee, Sang Wook LU ; Lee, Jong Hyun; Kwon, Hyuck Gi; Laurell, Thomas LU ; Jeong, Ok Chan and Kim, Soyoun (2018) In Analytical Sciences 34(3). p.317-321
Abstract

Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z =... (More)

Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z = 1272, 1407, and 1872). The sol-gel microarray platform enables dual readout of PSA both fluorometric and MALDI-TOF MS analysis in biological samples. Here we report a useful method for a means for discovery of biomarkers in complex body fluids.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biomarker discovery, Macro-structure silicon, MALDI-TOF mass spectrometry, PSA, Sensitive diagnosis, Sol-gel microarray
in
Analytical Sciences
volume
34
issue
3
pages
5 pages
publisher
Japan Society for Analytical Chemistry
external identifiers
  • scopus:85043372493
ISSN
0910-6340
DOI
10.2116/analsci.34.317
language
English
LU publication?
yes
id
b4307f54-d99c-43fc-b91f-af51131c294a
date added to LUP
2018-03-22 15:07:37
date last changed
2019-03-19 03:51:34
@article{b4307f54-d99c-43fc-b91f-af51131c294a,
  abstract     = {<p>Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z = 1272, 1407, and 1872). The sol-gel microarray platform enables dual readout of PSA both fluorometric and MALDI-TOF MS analysis in biological samples. Here we report a useful method for a means for discovery of biomarkers in complex body fluids.</p>},
  author       = {Lee, Sang Wook and Lee, Jong Hyun and Kwon, Hyuck Gi and Laurell, Thomas and Jeong, Ok Chan and Kim, Soyoun},
  issn         = {0910-6340},
  keyword      = {Biomarker discovery,Macro-structure silicon,MALDI-TOF mass spectrometry,PSA,Sensitive diagnosis,Sol-gel microarray},
  language     = {eng},
  number       = {3},
  pages        = {317--321},
  publisher    = {Japan Society for Analytical Chemistry},
  series       = {Analytical Sciences},
  title        = {A sol-gel integrated dual-readout microarray platform for quantification and identification of prostate-specific antigen},
  url          = {http://dx.doi.org/10.2116/analsci.34.317},
  volume       = {34},
  year         = {2018},
}