Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

A sol-gel integrated dual-readout microarray platform for quantification and identification of prostate-specific antigen

Lee, Sang Wook LU ; Lee, Jong Hyun ; Kwon, Hyuck Gi ; Laurell, Thomas LU ; Jeong, Ok Chan and Kim, Soyoun (2018) In Analytical Sciences 34(3). p.317-321
Abstract

Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z =... (More)

Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z = 1272, 1407, and 1872). The sol-gel microarray platform enables dual readout of PSA both fluorometric and MALDI-TOF MS analysis in biological samples. Here we report a useful method for a means for discovery of biomarkers in complex body fluids.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biomarker discovery, Macro-structure silicon, MALDI-TOF mass spectrometry, PSA, Sensitive diagnosis, Sol-gel microarray
in
Analytical Sciences
volume
34
issue
3
pages
5 pages
publisher
Japan Society for Analytical Chemistry
external identifiers
  • pmid:29526899
  • scopus:85043372493
ISSN
0910-6340
DOI
10.2116/analsci.34.317
language
English
LU publication?
yes
id
b4307f54-d99c-43fc-b91f-af51131c294a
date added to LUP
2018-03-22 15:07:37
date last changed
2022-04-25 06:26:49
@article{b4307f54-d99c-43fc-b91f-af51131c294a,
  abstract     = {{<p>Here, we report a sol-gel integrated affinity microarray for on-chip matrix-assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS) that enables capture and identification of prostate-specific antigen (PSA) in samples. An anti-PSA antibody (H117) was mixed with a sol-gel, and the mixture was spotted onto a porous silicon (pSi) surface without additional surface modifications. The antibody easily penetrates the sol-gel macropore fluidic network structure, making possible high affinities. To assess the capture affinity of the platform, we performed a direct assay using fluorescein isothiocyanate-labeled PSA. Pure PSA was subjected to on-chip MALDI-TOF-MS analysis, yielding three clear mass peptide peaks (m/z = 1272, 1407, and 1872). The sol-gel microarray platform enables dual readout of PSA both fluorometric and MALDI-TOF MS analysis in biological samples. Here we report a useful method for a means for discovery of biomarkers in complex body fluids.</p>}},
  author       = {{Lee, Sang Wook and Lee, Jong Hyun and Kwon, Hyuck Gi and Laurell, Thomas and Jeong, Ok Chan and Kim, Soyoun}},
  issn         = {{0910-6340}},
  keywords     = {{Biomarker discovery; Macro-structure silicon; MALDI-TOF mass spectrometry; PSA; Sensitive diagnosis; Sol-gel microarray}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{317--321}},
  publisher    = {{Japan Society for Analytical Chemistry}},
  series       = {{Analytical Sciences}},
  title        = {{A sol-gel integrated dual-readout microarray platform for quantification and identification of prostate-specific antigen}},
  url          = {{http://dx.doi.org/10.2116/analsci.34.317}},
  doi          = {{10.2116/analsci.34.317}},
  volume       = {{34}},
  year         = {{2018}},
}