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Following Protein Dynamics in Real Time during Crystallization

Beck, Christian ; Grimaldo, Marco ; Roosen-Runge, Felix LU ; Maier, Ralph ; Matsarskaia, Olga ; Braun, Michal ; Sohmen, Benedikt ; Czakkel, Orsolya ; Schweins, Ralf and Zhang, Fajun , et al. (2019) In Crystal Growth and Design p.7036-7045
Abstract

The process of protein crystallization from aqueous protein solutions is still insufficiently understood. During macroscopic crystal formation, occurring often on time scales from a few hours to several days, protein dynamics evolves on the molecular level. Here, we present a proof of concept and a framework to observe this evolving diffusive dynamics on the pico- to nanosecond time scale, associated with cluster or precursor formation that ultimately results in emerging crystals. We investigated the model system of the protein β-lactoglobulin in D2O in the presence of ZnCl2, which induces crystallization by electrostatic bridges. First, the structural changes occurring during crystallization were followed by... (More)

The process of protein crystallization from aqueous protein solutions is still insufficiently understood. During macroscopic crystal formation, occurring often on time scales from a few hours to several days, protein dynamics evolves on the molecular level. Here, we present a proof of concept and a framework to observe this evolving diffusive dynamics on the pico- to nanosecond time scale, associated with cluster or precursor formation that ultimately results in emerging crystals. We investigated the model system of the protein β-lactoglobulin in D2O in the presence of ZnCl2, which induces crystallization by electrostatic bridges. First, the structural changes occurring during crystallization were followed by small-angle neutron scattering. Furthermore, we employed neutron backscattering and spin-echo spectroscopy to measure the ensemble-averaged self- and collective diffusion on nanosecond time scales of protein solutions with a kinetic time resolution on the order of 15 min. The experiments provide information on the increasing number fraction of immobilized proteins as well as on the diffusive motion of unbound proteins in an increasingly depleted phase. Simultaneously, information on the internal dynamics of the proteins is obtained.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Crystal Growth and Design
pages
7036 - 7045
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85075447766
ISSN
1528-7483
DOI
10.1021/acs.cgd.9b00858
language
English
LU publication?
yes
id
b46ce0ab-2db6-4e6c-ada6-02f42071eb36
date added to LUP
2019-12-09 13:29:40
date last changed
2022-04-18 19:22:57
@article{b46ce0ab-2db6-4e6c-ada6-02f42071eb36,
  abstract     = {{<p>The process of protein crystallization from aqueous protein solutions is still insufficiently understood. During macroscopic crystal formation, occurring often on time scales from a few hours to several days, protein dynamics evolves on the molecular level. Here, we present a proof of concept and a framework to observe this evolving diffusive dynamics on the pico- to nanosecond time scale, associated with cluster or precursor formation that ultimately results in emerging crystals. We investigated the model system of the protein β-lactoglobulin in D<sub>2</sub>O in the presence of ZnCl<sub>2</sub>, which induces crystallization by electrostatic bridges. First, the structural changes occurring during crystallization were followed by small-angle neutron scattering. Furthermore, we employed neutron backscattering and spin-echo spectroscopy to measure the ensemble-averaged self- and collective diffusion on nanosecond time scales of protein solutions with a kinetic time resolution on the order of 15 min. The experiments provide information on the increasing number fraction of immobilized proteins as well as on the diffusive motion of unbound proteins in an increasingly depleted phase. Simultaneously, information on the internal dynamics of the proteins is obtained.</p>}},
  author       = {{Beck, Christian and Grimaldo, Marco and Roosen-Runge, Felix and Maier, Ralph and Matsarskaia, Olga and Braun, Michal and Sohmen, Benedikt and Czakkel, Orsolya and Schweins, Ralf and Zhang, Fajun and Seydel, Tilo and Schreiber, Frank}},
  issn         = {{1528-7483}},
  language     = {{eng}},
  pages        = {{7036--7045}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Crystal Growth and Design}},
  title        = {{Following Protein Dynamics in Real Time during Crystallization}},
  url          = {{http://dx.doi.org/10.1021/acs.cgd.9b00858}},
  doi          = {{10.1021/acs.cgd.9b00858}},
  year         = {{2019}},
}