Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Longitudinal Imaging Using PET/CT with Collagen-I PET-Tracer and MRI for Assessment of Fibrotic and Inflammatory Lesions in a Rat Lung Injury Model

Mahmutovic Persson, Irma LU ; Fransén Pettersson, Nina LU ; Liu, Jian LU ; Falk Håkansson, Hanna ; Örbom, Anders LU ; In 't Zandt, René LU orcid ; Gidlöf, Ritha LU ; Sydoff, Marie LU ; von Wachenfeldt, Karin and Olsson, Lars E LU orcid (2020) In Journal of Clinical Medicine 9(11). p.1-21
Abstract

Non-invasive imaging biomarkers (IBs) are warranted to enable improved diagnostics and follow-up monitoring of interstitial lung disease (ILD) including drug-induced ILD (DIILD). Of special interest are IB, which can characterize and differentiate acute inflammation from fibrosis. The aim of the present study was to evaluate a PET-tracer specific for Collagen-I, combined with multi-echo MRI, in a rat model of DIILD. Rats were challenged intratracheally with bleomycin, and subsequently followed by MRI and PET/CT for four weeks. PET imaging demonstrated a significantly increased uptake of the collagen tracer in the lungs of challenged rats compared to controls. This was confirmed by MRI characterization of the lesions as edema or fibrotic... (More)

Non-invasive imaging biomarkers (IBs) are warranted to enable improved diagnostics and follow-up monitoring of interstitial lung disease (ILD) including drug-induced ILD (DIILD). Of special interest are IB, which can characterize and differentiate acute inflammation from fibrosis. The aim of the present study was to evaluate a PET-tracer specific for Collagen-I, combined with multi-echo MRI, in a rat model of DIILD. Rats were challenged intratracheally with bleomycin, and subsequently followed by MRI and PET/CT for four weeks. PET imaging demonstrated a significantly increased uptake of the collagen tracer in the lungs of challenged rats compared to controls. This was confirmed by MRI characterization of the lesions as edema or fibrotic tissue. The uptake of tracer did not show complete spatial overlap with the lesions identified by MRI. Instead, the tracer signal appeared at the borderline between lesion and healthy tissue. Histological tissue staining, fibrosis scoring, lysyl oxidase activity measurements, and gene expression markers all confirmed establishing fibrosis over time. In conclusion, the novel PET tracer for Collagen-I combined with multi-echo MRI, were successfully able to monitor fibrotic changes in bleomycin-induced lung injury. The translational approach of using non-invasive imaging techniques show potential also from a clinical perspective.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
author collaboration
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Medicine
volume
9
issue
11
pages
1 - 21
publisher
MDPI AG
external identifiers
  • pmid:33218212
  • scopus:85114070588
ISSN
2077-0383
DOI
10.3390/jcm9113706
language
English
LU publication?
yes
id
b482bc9d-c6d0-4be2-b7aa-5954ef96cb92
date added to LUP
2021-01-21 08:23:54
date last changed
2024-05-30 05:02:55
@article{b482bc9d-c6d0-4be2-b7aa-5954ef96cb92,
  abstract     = {{<p>Non-invasive imaging biomarkers (IBs) are warranted to enable improved diagnostics and follow-up monitoring of interstitial lung disease (ILD) including drug-induced ILD (DIILD). Of special interest are IB, which can characterize and differentiate acute inflammation from fibrosis. The aim of the present study was to evaluate a PET-tracer specific for Collagen-I, combined with multi-echo MRI, in a rat model of DIILD. Rats were challenged intratracheally with bleomycin, and subsequently followed by MRI and PET/CT for four weeks. PET imaging demonstrated a significantly increased uptake of the collagen tracer in the lungs of challenged rats compared to controls. This was confirmed by MRI characterization of the lesions as edema or fibrotic tissue. The uptake of tracer did not show complete spatial overlap with the lesions identified by MRI. Instead, the tracer signal appeared at the borderline between lesion and healthy tissue. Histological tissue staining, fibrosis scoring, lysyl oxidase activity measurements, and gene expression markers all confirmed establishing fibrosis over time. In conclusion, the novel PET tracer for Collagen-I combined with multi-echo MRI, were successfully able to monitor fibrotic changes in bleomycin-induced lung injury. The translational approach of using non-invasive imaging techniques show potential also from a clinical perspective.</p>}},
  author       = {{Mahmutovic Persson, Irma and Fransén Pettersson, Nina and Liu, Jian and Falk Håkansson, Hanna and Örbom, Anders and In 't Zandt, René and Gidlöf, Ritha and Sydoff, Marie and von Wachenfeldt, Karin and Olsson, Lars E}},
  issn         = {{2077-0383}},
  language     = {{eng}},
  month        = {{11}},
  number       = {{11}},
  pages        = {{1--21}},
  publisher    = {{MDPI AG}},
  series       = {{Journal of Clinical Medicine}},
  title        = {{Longitudinal Imaging Using PET/CT with Collagen-I PET-Tracer and MRI for Assessment of Fibrotic and Inflammatory Lesions in a Rat Lung Injury Model}},
  url          = {{http://dx.doi.org/10.3390/jcm9113706}},
  doi          = {{10.3390/jcm9113706}},
  volume       = {{9}},
  year         = {{2020}},
}