Longitudinal Imaging Using PET/CT with Collagen-I PET-Tracer and MRI for Assessment of Fibrotic and Inflammatory Lesions in a Rat Lung Injury Model
(2020) In Journal of Clinical Medicine 9(11). p.1-21- Abstract
Non-invasive imaging biomarkers (IBs) are warranted to enable improved diagnostics and follow-up monitoring of interstitial lung disease (ILD) including drug-induced ILD (DIILD). Of special interest are IB, which can characterize and differentiate acute inflammation from fibrosis. The aim of the present study was to evaluate a PET-tracer specific for Collagen-I, combined with multi-echo MRI, in a rat model of DIILD. Rats were challenged intratracheally with bleomycin, and subsequently followed by MRI and PET/CT for four weeks. PET imaging demonstrated a significantly increased uptake of the collagen tracer in the lungs of challenged rats compared to controls. This was confirmed by MRI characterization of the lesions as edema or fibrotic... (More)
Non-invasive imaging biomarkers (IBs) are warranted to enable improved diagnostics and follow-up monitoring of interstitial lung disease (ILD) including drug-induced ILD (DIILD). Of special interest are IB, which can characterize and differentiate acute inflammation from fibrosis. The aim of the present study was to evaluate a PET-tracer specific for Collagen-I, combined with multi-echo MRI, in a rat model of DIILD. Rats were challenged intratracheally with bleomycin, and subsequently followed by MRI and PET/CT for four weeks. PET imaging demonstrated a significantly increased uptake of the collagen tracer in the lungs of challenged rats compared to controls. This was confirmed by MRI characterization of the lesions as edema or fibrotic tissue. The uptake of tracer did not show complete spatial overlap with the lesions identified by MRI. Instead, the tracer signal appeared at the borderline between lesion and healthy tissue. Histological tissue staining, fibrosis scoring, lysyl oxidase activity measurements, and gene expression markers all confirmed establishing fibrosis over time. In conclusion, the novel PET tracer for Collagen-I combined with multi-echo MRI, were successfully able to monitor fibrotic changes in bleomycin-induced lung injury. The translational approach of using non-invasive imaging techniques show potential also from a clinical perspective.
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- author
- Mahmutovic Persson, Irma LU ; Fransén Pettersson, Nina LU ; Liu, Jian LU ; Falk Håkansson, Hanna ; Örbom, Anders LU ; In 't Zandt, René LU ; Gidlöf, Ritha LU ; Sydoff, Marie LU ; von Wachenfeldt, Karin and Olsson, Lars E LU
- author collaboration
- organization
- publishing date
- 2020-11-18
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Medicine
- volume
- 9
- issue
- 11
- pages
- 1 - 21
- publisher
- MDPI AG
- external identifiers
-
- pmid:33218212
- scopus:85114070588
- ISSN
- 2077-0383
- DOI
- 10.3390/jcm9113706
- language
- English
- LU publication?
- yes
- id
- b482bc9d-c6d0-4be2-b7aa-5954ef96cb92
- date added to LUP
- 2021-01-21 08:23:54
- date last changed
- 2024-09-19 15:09:18
@article{b482bc9d-c6d0-4be2-b7aa-5954ef96cb92, abstract = {{<p>Non-invasive imaging biomarkers (IBs) are warranted to enable improved diagnostics and follow-up monitoring of interstitial lung disease (ILD) including drug-induced ILD (DIILD). Of special interest are IB, which can characterize and differentiate acute inflammation from fibrosis. The aim of the present study was to evaluate a PET-tracer specific for Collagen-I, combined with multi-echo MRI, in a rat model of DIILD. Rats were challenged intratracheally with bleomycin, and subsequently followed by MRI and PET/CT for four weeks. PET imaging demonstrated a significantly increased uptake of the collagen tracer in the lungs of challenged rats compared to controls. This was confirmed by MRI characterization of the lesions as edema or fibrotic tissue. The uptake of tracer did not show complete spatial overlap with the lesions identified by MRI. Instead, the tracer signal appeared at the borderline between lesion and healthy tissue. Histological tissue staining, fibrosis scoring, lysyl oxidase activity measurements, and gene expression markers all confirmed establishing fibrosis over time. In conclusion, the novel PET tracer for Collagen-I combined with multi-echo MRI, were successfully able to monitor fibrotic changes in bleomycin-induced lung injury. The translational approach of using non-invasive imaging techniques show potential also from a clinical perspective.</p>}}, author = {{Mahmutovic Persson, Irma and Fransén Pettersson, Nina and Liu, Jian and Falk Håkansson, Hanna and Örbom, Anders and In 't Zandt, René and Gidlöf, Ritha and Sydoff, Marie and von Wachenfeldt, Karin and Olsson, Lars E}}, issn = {{2077-0383}}, language = {{eng}}, month = {{11}}, number = {{11}}, pages = {{1--21}}, publisher = {{MDPI AG}}, series = {{Journal of Clinical Medicine}}, title = {{Longitudinal Imaging Using PET/CT with Collagen-I PET-Tracer and MRI for Assessment of Fibrotic and Inflammatory Lesions in a Rat Lung Injury Model}}, url = {{http://dx.doi.org/10.3390/jcm9113706}}, doi = {{10.3390/jcm9113706}}, volume = {{9}}, year = {{2020}}, }