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Poly(ester amide) microspheres are efficient vehicles for long-term intracerebral growth factor delivery and improve functional recovery after stroke

Memanishvili, Tamar LU ; Monni, Emanuela LU ; Tatarishivili, Jemal LU ; Lindvall, Olle LU ; Tsiskaridze, Alexander ; Kokaia, Zaal LU and Tornero, Daniel LU (2020) In Biomedical materials (Bristol, England) 15(6).
Abstract

Growth factors promote plasticity in injured brain and improve impaired functions. For clinical application, efficient approaches for growth factor delivery into the brain are necessary. Poly(ester amide) (PEA)-derived microspheres (MS) could serve as vehicles due to their thermal and mechanical properties, biocompatibility and biodegradability. Vascular endothelial growth factor (VEGF) exerts both vascular and neuronal actions, making it suitable to stimulate post-stroke recovery. Here, PEA (composed of adipic acid, L-phenyl-alanine and 1,4-butanediol) MS were loaded with VEGF and injected intracerebrally in mice subjected to cortical stroke. Loaded MS provided sustained release of VEGF in vitro and, after injection, biologically... (More)

Growth factors promote plasticity in injured brain and improve impaired functions. For clinical application, efficient approaches for growth factor delivery into the brain are necessary. Poly(ester amide) (PEA)-derived microspheres (MS) could serve as vehicles due to their thermal and mechanical properties, biocompatibility and biodegradability. Vascular endothelial growth factor (VEGF) exerts both vascular and neuronal actions, making it suitable to stimulate post-stroke recovery. Here, PEA (composed of adipic acid, L-phenyl-alanine and 1,4-butanediol) MS were loaded with VEGF and injected intracerebrally in mice subjected to cortical stroke. Loaded MS provided sustained release of VEGF in vitro and, after injection, biologically active VEGF was released long-term, as evidenced by high VEGF immunoreactivity, increased VEGF tissue levels, and higher vessel density and more NG2+ cells in injured hemisphere of animals with VEGF-loaded as compared to non-loaded MS. Loaded MS gave rise to more rapid recovery of neurological score. Both loaded and non-loaded MS induced improvement in neurological score and adhesive removal test, probably due to anti-inflammatory action. In summary, grafted PEA MS can act as efficient vehicles, with anti-inflammatory action, for long-term delivery of growth factors into injured brain. Our data suggest PEA MS as a new tool for neurorestorative approaches with therapeutic potential.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomedical materials (Bristol, England)
volume
15
issue
6
article number
65020
publisher
IOP Publishing
external identifiers
  • pmid:32650328
  • scopus:85096885457
ISSN
1748-6041
DOI
10.1088/1748-605X/aba4f6
language
English
LU publication?
yes
id
b52491b8-1e7c-48a2-a46e-bdae0db96796
date added to LUP
2020-12-11 10:26:22
date last changed
2021-03-03 02:18:56
@article{b52491b8-1e7c-48a2-a46e-bdae0db96796,
  abstract     = {<p>Growth factors promote plasticity in injured brain and improve impaired functions. For clinical application, efficient approaches for growth factor delivery into the brain are necessary. Poly(ester amide) (PEA)-derived microspheres (MS) could serve as vehicles due to their thermal and mechanical properties, biocompatibility and biodegradability. Vascular endothelial growth factor (VEGF) exerts both vascular and neuronal actions, making it suitable to stimulate post-stroke recovery. Here, PEA (composed of adipic acid, L-phenyl-alanine and 1,4-butanediol) MS were loaded with VEGF and injected intracerebrally in mice subjected to cortical stroke. Loaded MS provided sustained release of VEGF in vitro and, after injection, biologically active VEGF was released long-term, as evidenced by high VEGF immunoreactivity, increased VEGF tissue levels, and higher vessel density and more NG2+ cells in injured hemisphere of animals with VEGF-loaded as compared to non-loaded MS. Loaded MS gave rise to more rapid recovery of neurological score. Both loaded and non-loaded MS induced improvement in neurological score and adhesive removal test, probably due to anti-inflammatory action. In summary, grafted PEA MS can act as efficient vehicles, with anti-inflammatory action, for long-term delivery of growth factors into injured brain. Our data suggest PEA MS as a new tool for neurorestorative approaches with therapeutic potential.</p>},
  author       = {Memanishvili, Tamar and Monni, Emanuela and Tatarishivili, Jemal and Lindvall, Olle and Tsiskaridze, Alexander and Kokaia, Zaal and Tornero, Daniel},
  issn         = {1748-6041},
  language     = {eng},
  number       = {6},
  publisher    = {IOP Publishing},
  series       = {Biomedical materials (Bristol, England)},
  title        = {Poly(ester amide) microspheres are efficient vehicles for long-term intracerebral growth factor delivery and improve functional recovery after stroke},
  url          = {http://dx.doi.org/10.1088/1748-605X/aba4f6},
  doi          = {10.1088/1748-605X/aba4f6},
  volume       = {15},
  year         = {2020},
}