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Glycolytic enzyme Enolase-1 regulates insulin gene expression in pancreatic β-cell

Luo, Xiumei ; Luan, Cheng LU ; Zhou, Jingqi ; Ye, Yingying LU ; Zhang, Wei ; Jain, Ruchi LU ; Zhang, Enming LU and Chen, Ning (2024) In Biochemical and Biophysical Research Communications 706.
Abstract

Enolase-1 (Eno1) plays a critical role in regulating glucose metabolism; however, its specific impact on pancreatic islet β-cells remains elusive. This study aimed to provide a preliminary exploration of Eno1 function in pancreatic islet β-cells. The findings revealed that the expression of ENO1 mRNA in type 2 diabetes donors was significantly increased and positively correlated with HbA1C and negatively correlated with insulin gene expression. A high level of Eno1 in human insulin-secreting rat INS-1832/13 cells with co-localization with intracellular insulin proteins was accordingly observed. Silencing of Eno1 using siRNA or inhibiting Eno1 protein activity with an Eno1 antagonist significantly reduced insulin secretion and insulin... (More)

Enolase-1 (Eno1) plays a critical role in regulating glucose metabolism; however, its specific impact on pancreatic islet β-cells remains elusive. This study aimed to provide a preliminary exploration of Eno1 function in pancreatic islet β-cells. The findings revealed that the expression of ENO1 mRNA in type 2 diabetes donors was significantly increased and positively correlated with HbA1C and negatively correlated with insulin gene expression. A high level of Eno1 in human insulin-secreting rat INS-1832/13 cells with co-localization with intracellular insulin proteins was accordingly observed. Silencing of Eno1 using siRNA or inhibiting Eno1 protein activity with an Eno1 antagonist significantly reduced insulin secretion and insulin content in β-cells, while the proinsulin/insulin content ratio remained unchanged. This reduction in β-cells function was accompanied by a notable decrease in intracellular ATP and mitochondrial cytochrome C levels. Overall, our findings confirm that Eno1 regulates the insulin secretion process, particularly glucose metabolism and ATP production in the β-cells. The mechanism primarily involves its influence on insulin production, suggesting that Eno1 represents a potential target for β-cell protection and diabetes treatment.

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ATP, Enolase-1, Glucose metabolism, β-cells function
in
Biochemical and Biophysical Research Communications
volume
706
article number
149735
publisher
Elsevier
external identifiers
  • scopus:85187340897
  • pmid:38461647
ISSN
0006-291X
DOI
10.1016/j.bbrc.2024.149735
language
English
LU publication?
yes
id
b52761f8-91a4-4f3f-a49a-253aeb6402f7
date added to LUP
2024-03-27 15:20:56
date last changed
2024-12-19 20:48:34
@article{b52761f8-91a4-4f3f-a49a-253aeb6402f7,
  abstract     = {{<p>Enolase-1 (Eno1) plays a critical role in regulating glucose metabolism; however, its specific impact on pancreatic islet β-cells remains elusive. This study aimed to provide a preliminary exploration of Eno1 function in pancreatic islet β-cells. The findings revealed that the expression of ENO1 mRNA in type 2 diabetes donors was significantly increased and positively correlated with HbA1C and negatively correlated with insulin gene expression. A high level of Eno1 in human insulin-secreting rat INS-1832/13 cells with co-localization with intracellular insulin proteins was accordingly observed. Silencing of Eno1 using siRNA or inhibiting Eno1 protein activity with an Eno1 antagonist significantly reduced insulin secretion and insulin content in β-cells, while the proinsulin/insulin content ratio remained unchanged. This reduction in β-cells function was accompanied by a notable decrease in intracellular ATP and mitochondrial cytochrome C levels. Overall, our findings confirm that Eno1 regulates the insulin secretion process, particularly glucose metabolism and ATP production in the β-cells. The mechanism primarily involves its influence on insulin production, suggesting that Eno1 represents a potential target for β-cell protection and diabetes treatment.</p>}},
  author       = {{Luo, Xiumei and Luan, Cheng and Zhou, Jingqi and Ye, Yingying and Zhang, Wei and Jain, Ruchi and Zhang, Enming and Chen, Ning}},
  issn         = {{0006-291X}},
  keywords     = {{ATP; Enolase-1; Glucose metabolism; β-cells function}},
  language     = {{eng}},
  month        = {{04}},
  publisher    = {{Elsevier}},
  series       = {{Biochemical and Biophysical Research Communications}},
  title        = {{Glycolytic enzyme Enolase-1 regulates insulin gene expression in pancreatic β-cell}},
  url          = {{http://dx.doi.org/10.1016/j.bbrc.2024.149735}},
  doi          = {{10.1016/j.bbrc.2024.149735}},
  volume       = {{706}},
  year         = {{2024}},
}