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Increased proportion of CD8+ tumor responsive T cells after immunization with tum- versus tum+ rat glioma

Siesjö, P LU orcid ; Visse, E LU and Sjögren, H O LU (1995) In Cellular Immunology 165(2). p.33-225
Abstract

Previously established immunogenic (tum-) clones of an ENU (ethyl-N-nitrosourea)-induced rat glioma, N32, were compared to the original tumor concerning their capacity to induce T lymphocyte responses after in vivo immunization and in vitro restimulation of responder spleen cells in mixed lymphocyte tumor culture (MLTC) assays. Quite unexpectedly, original N32 (tum+) in vivo primed spleen cells proliferated to the same extent in vitro in response to tum+ stimulator cells as did tum- in vivo primed spleen cells. However, flow-cytometric analysis of parallel cultures showed a greatly increased proportion of CD3+CD8+ lymphocytes in the proliferating responder cell population from tum- immunized hosts, contrary to a CD3+CD4+ lymphocyte... (More)

Previously established immunogenic (tum-) clones of an ENU (ethyl-N-nitrosourea)-induced rat glioma, N32, were compared to the original tumor concerning their capacity to induce T lymphocyte responses after in vivo immunization and in vitro restimulation of responder spleen cells in mixed lymphocyte tumor culture (MLTC) assays. Quite unexpectedly, original N32 (tum+) in vivo primed spleen cells proliferated to the same extent in vitro in response to tum+ stimulator cells as did tum- in vivo primed spleen cells. However, flow-cytometric analysis of parallel cultures showed a greatly increased proportion of CD3+CD8+ lymphocytes in the proliferating responder cell population from tum- immunized hosts, contrary to a CD3+CD4+ lymphocyte dominance after tum+ immunization. Although the original tum+ N32 tumor cells are not capable of inducing a clearly demonstrable isograft rejection response, they induce a strong T cell response readily detectable in MLTC assays. We propose that the increased CD8+ lymphocyte proliferation could be an essential feature of the isograft rejection response induced by tum- tumor variants. Possible mechanisms of the augmented CD8+ T cell response are discussed.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, CD8-Positive T-Lymphocytes/immunology, Flow Cytometry, Glioma/immunology, Graft Rejection, Immunization, Lymphocyte Activation, Rats, Rats, Inbred F344, Transplantation, Isogeneic, Tumor Cells, Cultured
in
Cellular Immunology
volume
165
issue
2
pages
9 pages
publisher
Elsevier
external identifiers
  • pmid:7553887
  • scopus:0028847190
ISSN
0008-8749
DOI
10.1006/cimm.1995.1209
language
English
LU publication?
yes
id
b54ce117-d0fd-47f8-b361-94b36a5e5412
date added to LUP
2019-06-27 10:22:00
date last changed
2024-01-01 13:37:26
@article{b54ce117-d0fd-47f8-b361-94b36a5e5412,
  abstract     = {{<p>Previously established immunogenic (tum-) clones of an ENU (ethyl-N-nitrosourea)-induced rat glioma, N32, were compared to the original tumor concerning their capacity to induce T lymphocyte responses after in vivo immunization and in vitro restimulation of responder spleen cells in mixed lymphocyte tumor culture (MLTC) assays. Quite unexpectedly, original N32 (tum+) in vivo primed spleen cells proliferated to the same extent in vitro in response to tum+ stimulator cells as did tum- in vivo primed spleen cells. However, flow-cytometric analysis of parallel cultures showed a greatly increased proportion of CD3+CD8+ lymphocytes in the proliferating responder cell population from tum- immunized hosts, contrary to a CD3+CD4+ lymphocyte dominance after tum+ immunization. Although the original tum+ N32 tumor cells are not capable of inducing a clearly demonstrable isograft rejection response, they induce a strong T cell response readily detectable in MLTC assays. We propose that the increased CD8+ lymphocyte proliferation could be an essential feature of the isograft rejection response induced by tum- tumor variants. Possible mechanisms of the augmented CD8+ T cell response are discussed.</p>}},
  author       = {{Siesjö, P and Visse, E and Sjögren, H O}},
  issn         = {{0008-8749}},
  keywords     = {{Animals; CD8-Positive T-Lymphocytes/immunology; Flow Cytometry; Glioma/immunology; Graft Rejection; Immunization; Lymphocyte Activation; Rats; Rats, Inbred F344; Transplantation, Isogeneic; Tumor Cells, Cultured}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{2}},
  pages        = {{33--225}},
  publisher    = {{Elsevier}},
  series       = {{Cellular Immunology}},
  title        = {{Increased proportion of CD8+ tumor responsive T cells after immunization with tum- versus tum+ rat glioma}},
  url          = {{http://dx.doi.org/10.1006/cimm.1995.1209}},
  doi          = {{10.1006/cimm.1995.1209}},
  volume       = {{165}},
  year         = {{1995}},
}