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Anti-Incretin Gut Features Induced by Feed Supplementation with Alpha-Amylase : Studies on EPI Pigs

Pierzynowska, Kateryna LU orcid ; Wychowański, Piotr ; Zaworski, Kamil ; Woliński, Jarosław LU ; Donaldson, Janine and Pierzynowski, Stefan LU (2023) In International Journal of Molecular Sciences 24(22).
Abstract

The acini-islet-acinar (AIA) axis concept justifies the anatomical placement of the Langerhans islets within the exocrine pancreatic parenchyma and explains the existence of the pancreas as a single organ. Amylase has been suggested to play a key role as an anti-incretin factor. Oral glucose tolerance tests (OGTT) were performed on 18 piglets in both a healthy (prior to pancreatic duct ligation (PDL) surgery, study Day 10) and an exocrine pancreatic insufficient (EPI) state (30 days after PDL, study Day 48)). Amylase (4000 units/feeding) or Creon® (100,000 units/feeding) was administered to pigs with the morning and evening meals, according to study design randomization, for 37 days following the first OGTT. Blood glucose... (More)

The acini-islet-acinar (AIA) axis concept justifies the anatomical placement of the Langerhans islets within the exocrine pancreatic parenchyma and explains the existence of the pancreas as a single organ. Amylase has been suggested to play a key role as an anti-incretin factor. Oral glucose tolerance tests (OGTT) were performed on 18 piglets in both a healthy (prior to pancreatic duct ligation (PDL) surgery, study Day 10) and an exocrine pancreatic insufficient (EPI) state (30 days after PDL, study Day 48)). Amylase (4000 units/feeding) or Creon® (100,000 units/feeding) was administered to pigs with the morning and evening meals, according to study design randomization, for 37 days following the first OGTT. Blood glucose levels, as well as plasma levels of insulin, GLP-1, and GIP, were measured, and the HOMA-IR index was calculated. EPI status did not affect the area under the curve (AUC) of insulin release, fasting insulin levels, or the HOMA-IR index, while amylase supplementation led to a significant (p < 0.05) decrease in the above-mentioned parameters. At the same time, EPI led to a significant (p < 0.05) increase in GLP-1 levels, and neither amylase nor Creon® supplementation had any effects on this EPI-related increase. Fasting plasma levels of GIP were not affected by EPI; however, the GIP response in EPI and Amylase-treated EPI animals was significantly lower (p < 0.05) when compared to that of the intact, healthy pigs. Orally administered amylase induces gut anti-incretin action, normalizing glucose homeostasis and reducing HOMA-IR as a long-term outcome, thus lowering the risk of diabetes type II development. Amylase has long-lasting anti-incretin effects, and one could consider the existence of a long-lasting gut memory for amylase, which decreases hyperinsulinemia and hyperglycemia for up to 16 h after the last exposure of the gut to amylase.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amylase, anti-incretin, EPI pigs, GIP, GLP-1, glucose metabolism, OGTT
in
International Journal of Molecular Sciences
volume
24
issue
22
article number
16177
publisher
MDPI AG
external identifiers
  • pmid:38003366
  • scopus:85178362909
ISSN
1661-6596
DOI
10.3390/ijms242216177
language
English
LU publication?
yes
id
b5961071-6168-4657-ba35-2d35db12a1a8
date added to LUP
2024-01-02 13:33:05
date last changed
2024-04-17 12:48:39
@article{b5961071-6168-4657-ba35-2d35db12a1a8,
  abstract     = {{<p>The acini-islet-acinar (AIA) axis concept justifies the anatomical placement of the Langerhans islets within the exocrine pancreatic parenchyma and explains the existence of the pancreas as a single organ. Amylase has been suggested to play a key role as an anti-incretin factor. Oral glucose tolerance tests (OGTT) were performed on 18 piglets in both a healthy (prior to pancreatic duct ligation (PDL) surgery, study Day 10) and an exocrine pancreatic insufficient (EPI) state (30 days after PDL, study Day 48)). Amylase (4000 units/feeding) or Creon<sup>®</sup> (100,000 units/feeding) was administered to pigs with the morning and evening meals, according to study design randomization, for 37 days following the first OGTT. Blood glucose levels, as well as plasma levels of insulin, GLP-1, and GIP, were measured, and the HOMA-IR index was calculated. EPI status did not affect the area under the curve (AUC) of insulin release, fasting insulin levels, or the HOMA-IR index, while amylase supplementation led to a significant (p &lt; 0.05) decrease in the above-mentioned parameters. At the same time, EPI led to a significant (p &lt; 0.05) increase in GLP-1 levels, and neither amylase nor Creon<sup>®</sup> supplementation had any effects on this EPI-related increase. Fasting plasma levels of GIP were not affected by EPI; however, the GIP response in EPI and Amylase-treated EPI animals was significantly lower (p &lt; 0.05) when compared to that of the intact, healthy pigs. Orally administered amylase induces gut anti-incretin action, normalizing glucose homeostasis and reducing HOMA-IR as a long-term outcome, thus lowering the risk of diabetes type II development. Amylase has long-lasting anti-incretin effects, and one could consider the existence of a long-lasting gut memory for amylase, which decreases hyperinsulinemia and hyperglycemia for up to 16 h after the last exposure of the gut to amylase.</p>}},
  author       = {{Pierzynowska, Kateryna and Wychowański, Piotr and Zaworski, Kamil and Woliński, Jarosław and Donaldson, Janine and Pierzynowski, Stefan}},
  issn         = {{1661-6596}},
  keywords     = {{amylase; anti-incretin; EPI pigs; GIP; GLP-1; glucose metabolism; OGTT}},
  language     = {{eng}},
  number       = {{22}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Anti-Incretin Gut Features Induced by Feed Supplementation with Alpha-Amylase : Studies on EPI Pigs}},
  url          = {{http://dx.doi.org/10.3390/ijms242216177}},
  doi          = {{10.3390/ijms242216177}},
  volume       = {{24}},
  year         = {{2023}},
}