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Aqueous two-phase partitioning for proteomic monitoring of cell surface biomarkers in human peripheral blood mononuclear cells

Everberg, Henrik LU ; Peterson Wulff, Ragna LU ; Rak, S ; Tjerneld, Folke LU and Emanuelsson, Cecilia LU orcid (2006) In Journal of Proteome Research 5(5). p.1168-1175
Abstract
For proteomic monitoring of processes such as allergy or inflammation an efficient pre-fractionation strategy is required. We isolated plasma membranes from human peripheral blood mononuclear (PBM) cells by aqueous two-phase partitioning. After 1DE combined with LC-MS/MS, several cell surface marker proteins and in total 60 different plasma membrane proteins (out of 84 identified proteins, i.e., 72%) were detected. Plasma membranes obtained were from only one human donor, the procedure is therefore applicable for individual patient screening.
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
proteomics, prefractionation, two-phase partitioning, aqueous, cell surface biomarkers, allergy, plasma membrane proteins
in
Journal of Proteome Research
volume
5
issue
5
pages
1168 - 1175
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000237390200015
  • pmid:16674106
  • scopus:33646554173
ISSN
1535-3893
DOI
10.1021/pr050469z
language
English
LU publication?
yes
id
b5c723a2-bfbb-4a84-8e39-70ac83a5e84d (old id 410030)
date added to LUP
2016-04-01 12:36:12
date last changed
2025-04-04 14:58:35
@article{b5c723a2-bfbb-4a84-8e39-70ac83a5e84d,
  abstract     = {{For proteomic monitoring of processes such as allergy or inflammation an efficient pre-fractionation strategy is required. We isolated plasma membranes from human peripheral blood mononuclear (PBM) cells by aqueous two-phase partitioning. After 1DE combined with LC-MS/MS, several cell surface marker proteins and in total 60 different plasma membrane proteins (out of 84 identified proteins, i.e., 72%) were detected. Plasma membranes obtained were from only one human donor, the procedure is therefore applicable for individual patient screening.}},
  author       = {{Everberg, Henrik and Peterson Wulff, Ragna and Rak, S and Tjerneld, Folke and Emanuelsson, Cecilia}},
  issn         = {{1535-3893}},
  keywords     = {{proteomics; prefractionation; two-phase partitioning; aqueous; cell surface biomarkers; allergy; plasma membrane proteins}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1168--1175}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Journal of Proteome Research}},
  title        = {{Aqueous two-phase partitioning for proteomic monitoring of cell surface biomarkers in human peripheral blood mononuclear cells}},
  url          = {{http://dx.doi.org/10.1021/pr050469z}},
  doi          = {{10.1021/pr050469z}},
  volume       = {{5}},
  year         = {{2006}},
}