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A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental Analysis

Batlokwa, Bareki Shima; Mokgadi, Janes; Majors, Ronald; Turner, Charlotta LU and Torto, Nelson (2013) In Journal of Chemistry
Abstract
A novel molecularly imprinted polymer (MIP) powder designed for the selective removal of interfering chlorophyll pigment from heavily pigmented green plant extracts during pesticide residue analysis or analysis for bioactives in natural product research is reported. The polymer powder imprinted with chlorophyll.. was synthesized by copolymerizing the functional and cross-linking monomers, methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA), respectively. During batch rebinding experiments, the MIP effectively removed chlorophyll from neat chlorophyll standards, green medicinal plants, and spinach extracts with determined absorbance as high as 2.501 absorbance units (Au) to as low as 0.084 Au after the optimum quantity of the... (More)
A novel molecularly imprinted polymer (MIP) powder designed for the selective removal of interfering chlorophyll pigment from heavily pigmented green plant extracts during pesticide residue analysis or analysis for bioactives in natural product research is reported. The polymer powder imprinted with chlorophyll.. was synthesized by copolymerizing the functional and cross-linking monomers, methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA), respectively. During batch rebinding experiments, the MIP effectively removed chlorophyll from neat chlorophyll standards, green medicinal plants, and spinach extracts with determined absorbance as high as 2.501 absorbance units (Au) to as low as 0.084 Au after the optimum quantity of the MIP (170 mg mL(-1)) was added as the adsorbent prior to instrumental analysis. The determined 0.084 Au absorbance was far below the absorbance of 2% chlorophyll (0.401 Au) regarded as the cut-off point for interfering chlorophyll. Thus the newly developed polymer presents itself as a suitable adsorbent for the selective removal of chlorophyll from heavily pigmented, chlorophyll containing extracts. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Chemistry
publisher
Hindawi Publishing Corporation
external identifiers
  • wos:000319924000001
  • scopus:84878729687
ISSN
2090-9071
DOI
10.1155/2013/540240
language
English
LU publication?
yes
id
b67f371b-12af-439e-8798-9c0b86676f1d (old id 3930445)
date added to LUP
2013-07-16 13:03:41
date last changed
2019-04-10 01:18:11
@article{b67f371b-12af-439e-8798-9c0b86676f1d,
  abstract     = {A novel molecularly imprinted polymer (MIP) powder designed for the selective removal of interfering chlorophyll pigment from heavily pigmented green plant extracts during pesticide residue analysis or analysis for bioactives in natural product research is reported. The polymer powder imprinted with chlorophyll.. was synthesized by copolymerizing the functional and cross-linking monomers, methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA), respectively. During batch rebinding experiments, the MIP effectively removed chlorophyll from neat chlorophyll standards, green medicinal plants, and spinach extracts with determined absorbance as high as 2.501 absorbance units (Au) to as low as 0.084 Au after the optimum quantity of the MIP (170 mg mL(-1)) was added as the adsorbent prior to instrumental analysis. The determined 0.084 Au absorbance was far below the absorbance of 2% chlorophyll (0.401 Au) regarded as the cut-off point for interfering chlorophyll. Thus the newly developed polymer presents itself as a suitable adsorbent for the selective removal of chlorophyll from heavily pigmented, chlorophyll containing extracts.},
  articleno    = {540240},
  author       = {Batlokwa, Bareki Shima and Mokgadi, Janes and Majors, Ronald and Turner, Charlotta and Torto, Nelson},
  issn         = {2090-9071},
  language     = {eng},
  publisher    = {Hindawi Publishing Corporation},
  series       = {Journal of Chemistry},
  title        = {A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental Analysis},
  url          = {http://dx.doi.org/10.1155/2013/540240},
  year         = {2013},
}