Colorimetric aptasensing of microcystin-LR using DNA-conjugated polydiacetylene
Zhang, Man LU ; Zhang, Qicheng LU and Ye, Lei LU
(2024)
In Analytical and Bioanalytical Chemistry
416(29).
p.7131-7140
- Abstract
Polydiacetylene (PDA) holds promise as a versatile material for biosensing applications due to its unique optical properties and self-assembly capabilities. In this study, we developed a colorimetric detection biosensor system utilizing PDA and aptamer for the detection of microcystin-LR (MC-LR), a potent hepatotoxin found in cyanobacteria-contaminated environments. The biosensor was constructed by immobilizing MC-LR-specific aptamer on magnetic beads, where the aptamer was hybridized with a urease-labelled complementary DNA (cDNA-urease). Upon binding MC-LR, the aptamer undergoes a conformational change to release cDNA-urease. The released cDNA-urease is subsequently captured by PDA bearing a single-stranded DNA (ssDNA). The enzymatic... (More)
Polydiacetylene (PDA) holds promise as a versatile material for biosensing applications due to its unique optical properties and self-assembly capabilities. In this study, we developed a colorimetric detection biosensor system utilizing PDA and aptamer for the detection of microcystin-LR (MC-LR), a potent hepatotoxin found in cyanobacteria-contaminated environments. The biosensor was constructed by immobilizing MC-LR-specific aptamer on magnetic beads, where the aptamer was hybridized with a urease-labelled complementary DNA (cDNA-urease). Upon binding MC-LR, the aptamer undergoes a conformational change to release cDNA-urease. The released cDNA-urease is subsequently captured by PDA bearing a single-stranded DNA (ssDNA). The enzymatic reaction triggers a distinctive color transition of PDA from blue to red. The results demonstrate exceptional sensitivity, with a linear detection range of 5–100 ng/mL and a limit of detection as low as 1 ng/mL. The practicability of the colorimetric method was demonstrated by detecting different levels of MC-LR in spiked water samples. The recoveries ranged from 77.3 to 102% and the color change, visible to the naked eye, underscores the practical utility for on-site applications. Selectivity for MC-LR over other microcystin variants (MC-RR and MC-YR) was confirmed. The colorimetric detection platform capitalizes on the properties of PDA and nucleic acid, offering a robust method for detecting small molecules with potential applications in environmental monitoring and public health. Graphical Abstract: (Figure presented.)
(Less)
- author
- Zhang, Man
LU
; Zhang, Qicheng
LU
and Ye, Lei
LU
- organization
- publishing date
- 2024-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Colorimetric detection, DNA aptamer, Microcystin-LR, Polydiacetylene
- in
- Analytical and Bioanalytical Chemistry
- volume
- 416
- issue
- 29
- pages
- 10 pages
- publisher
- Springer Science and Business Media B.V.
- external identifiers
-
- scopus:85207323371
- pmid:39467911
- ISSN
- 1618-2642
- DOI
- 10.1007/s00216-024-05617-x
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © The Author(s) 2024.
- id
- b68bbedd-9c06-42ff-bdf1-5b2cbc916830
- date added to LUP
- 2024-12-18 12:48:51
- date last changed
- 2025-07-17 05:54:39
@article{b68bbedd-9c06-42ff-bdf1-5b2cbc916830,
abstract = {{<p>Polydiacetylene (PDA) holds promise as a versatile material for biosensing applications due to its unique optical properties and self-assembly capabilities. In this study, we developed a colorimetric detection biosensor system utilizing PDA and aptamer for the detection of microcystin-LR (MC-LR), a potent hepatotoxin found in cyanobacteria-contaminated environments. The biosensor was constructed by immobilizing MC-LR-specific aptamer on magnetic beads, where the aptamer was hybridized with a urease-labelled complementary DNA (cDNA-urease). Upon binding MC-LR, the aptamer undergoes a conformational change to release cDNA-urease. The released cDNA-urease is subsequently captured by PDA bearing a single-stranded DNA (ssDNA). The enzymatic reaction triggers a distinctive color transition of PDA from blue to red. The results demonstrate exceptional sensitivity, with a linear detection range of 5–100 ng/mL and a limit of detection as low as 1 ng/mL. The practicability of the colorimetric method was demonstrated by detecting different levels of MC-LR in spiked water samples. The recoveries ranged from 77.3 to 102% and the color change, visible to the naked eye, underscores the practical utility for on-site applications. Selectivity for MC-LR over other microcystin variants (MC-RR and MC-YR) was confirmed. The colorimetric detection platform capitalizes on the properties of PDA and nucleic acid, offering a robust method for detecting small molecules with potential applications in environmental monitoring and public health. Graphical Abstract: (Figure presented.)</p>}},
author = {{Zhang, Man and Zhang, Qicheng and Ye, Lei}},
issn = {{1618-2642}},
keywords = {{Colorimetric detection; DNA aptamer; Microcystin-LR; Polydiacetylene}},
language = {{eng}},
number = {{29}},
pages = {{7131--7140}},
publisher = {{Springer Science and Business Media B.V.}},
series = {{Analytical and Bioanalytical Chemistry}},
title = {{Colorimetric aptasensing of microcystin-LR using DNA-conjugated polydiacetylene}},
url = {{http://dx.doi.org/10.1007/s00216-024-05617-x}},
doi = {{10.1007/s00216-024-05617-x}},
volume = {{416}},
year = {{2024}},
}