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T-cell receptor repertoire in human germinal centres

Källberg, E LU ; Leanderson, T LU and Natvig, J B (2001) In Scandinavian Journal of Immunology 54(3). p.9-273
Abstract

A search for an antigen-driven expansion of T lymphocytes in the inflamed joints in rheumatoid arthritis (RA) patients have been going on for decades. We here analyzed the human germinal centre T-cell receptor (TCR) Vbeta gene usage with polymerase chain reaction (PCR) combined with sequence analysis, to address the question of clonality in tonsils and synovial tissue from RA patients. Our data show a large degree of TCR heterogeneity in both these histological structures. Furthermore, clonally related T cells were found within different closely located germinal centres indicating either an active T-cell migration between germinal centres (GC) or that a T-cell clone may seed more than one GC.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Arthritis, Rheumatoid/immunology, Base Sequence, CD57 Antigens/analysis, Clone Cells, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Genes, T-Cell Receptor beta, Germinal Center/cytology, Humans, Immunoglobulin Variable Region, Molecular Sequence Data, Palatine Tonsil/immunology, Polymerase Chain Reaction, Synovial Membrane/immunology, T-Lymphocytes/chemistry
in
Scandinavian Journal of Immunology
volume
54
issue
3
pages
9 - 273
publisher
Wiley-Blackwell
external identifiers
  • pmid:11555390
  • scopus:0035723153
ISSN
0300-9475
DOI
10.1046/j.1365-3083.2001.00898.x
language
English
LU publication?
yes
id
b6ad440a-c6a2-468f-97e3-2b972957ef9e
date added to LUP
2021-10-07 10:48:09
date last changed
2022-04-28 13:54:41
@article{b6ad440a-c6a2-468f-97e3-2b972957ef9e,
  abstract     = {{<p>A search for an antigen-driven expansion of T lymphocytes in the inflamed joints in rheumatoid arthritis (RA) patients have been going on for decades. We here analyzed the human germinal centre T-cell receptor (TCR) Vbeta gene usage with polymerase chain reaction (PCR) combined with sequence analysis, to address the question of clonality in tonsils and synovial tissue from RA patients. Our data show a large degree of TCR heterogeneity in both these histological structures. Furthermore, clonally related T cells were found within different closely located germinal centres indicating either an active T-cell migration between germinal centres (GC) or that a T-cell clone may seed more than one GC.</p>}},
  author       = {{Källberg, E and Leanderson, T and Natvig, J B}},
  issn         = {{0300-9475}},
  keywords     = {{Arthritis, Rheumatoid/immunology; Base Sequence; CD57 Antigens/analysis; Clone Cells; Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; Genes, T-Cell Receptor beta; Germinal Center/cytology; Humans; Immunoglobulin Variable Region; Molecular Sequence Data; Palatine Tonsil/immunology; Polymerase Chain Reaction; Synovial Membrane/immunology; T-Lymphocytes/chemistry}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{9--273}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Scandinavian Journal of Immunology}},
  title        = {{T-cell receptor repertoire in human germinal centres}},
  url          = {{http://dx.doi.org/10.1046/j.1365-3083.2001.00898.x}},
  doi          = {{10.1046/j.1365-3083.2001.00898.x}},
  volume       = {{54}},
  year         = {{2001}},
}