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High-resolution neutron spectroscopy on protein solution samples

Grimaldo, Marco ; Roosen-Runge, Felix LU ; Jalarvo, Niina ; Zamponi, Michaela ; Zanini, Fabio ; Hennig, Marcus ; Zhang, Fajun ; Schreiber, Frank and Seydel, Tilo (2015) 11th International Conference on Quasielastic Neutron Scattering, QENS 2014 and 6th International Workshop on Inelastic Neutron Spectrometers, WINS 2014 In EPJ Web of Conferences 83.
Abstract

Proteins in solution move subject to a complex superposition of global translational and rotational diffusion as well as internal relaxations covering a wide range of time scales. With the advent of new high-flux neutron spectrometers in combination with enhanced analysis frameworks it has become possible to separate these different contributions. We discuss new approaches to the analysis by presenting example spectra and fits from data recorded on the backscattering spectrometers IN16, IN16B, and BASIS on the same protein solution sample. We illustrate the separation of the rotational and translational diffusion contribution, the accurate treatment of the solvent contribution, and the extraction of information on internal fluctuations.... (More)

Proteins in solution move subject to a complex superposition of global translational and rotational diffusion as well as internal relaxations covering a wide range of time scales. With the advent of new high-flux neutron spectrometers in combination with enhanced analysis frameworks it has become possible to separate these different contributions. We discuss new approaches to the analysis by presenting example spectra and fits from data recorded on the backscattering spectrometers IN16, IN16B, and BASIS on the same protein solution sample. We illustrate the separation of the rotational and translational diffusion contribution, the accurate treatment of the solvent contribution, and the extraction of information on internal fluctuations. We also exemplify the progress made in passing from second- to third-generation backscattering spectrometers.

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author
; ; ; ; ; ; ; and
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
11th International Conference on Quasielastic Neutron Scattering, QENS 2014 and 6th International Workshop on Inelastic Neutron Spectrometers, WINS 2014
series title
EPJ Web of Conferences
volume
83
article number
02005
conference name
11th International Conference on Quasielastic Neutron Scattering, QENS 2014 and 6th International Workshop on Inelastic Neutron Spectrometers, WINS 2014
conference location
Autrans, France
conference dates
2014-05-11 - 2014-05-16
external identifiers
  • scopus:84921820321
ISSN
2101-6275
DOI
10.1051/epjconf/20158302005
language
English
LU publication?
no
id
b70f23c4-6f25-40c1-bc2a-f563ac353f92
date added to LUP
2018-12-17 09:44:40
date last changed
2022-01-31 20:38:04
@inproceedings{b70f23c4-6f25-40c1-bc2a-f563ac353f92,
  abstract     = {{<p>Proteins in solution move subject to a complex superposition of global translational and rotational diffusion as well as internal relaxations covering a wide range of time scales. With the advent of new high-flux neutron spectrometers in combination with enhanced analysis frameworks it has become possible to separate these different contributions. We discuss new approaches to the analysis by presenting example spectra and fits from data recorded on the backscattering spectrometers IN16, IN16B, and BASIS on the same protein solution sample. We illustrate the separation of the rotational and translational diffusion contribution, the accurate treatment of the solvent contribution, and the extraction of information on internal fluctuations. We also exemplify the progress made in passing from second- to third-generation backscattering spectrometers.</p>}},
  author       = {{Grimaldo, Marco and Roosen-Runge, Felix and Jalarvo, Niina and Zamponi, Michaela and Zanini, Fabio and Hennig, Marcus and Zhang, Fajun and Schreiber, Frank and Seydel, Tilo}},
  booktitle    = {{11th International Conference on Quasielastic Neutron Scattering, QENS 2014 and 6th International Workshop on Inelastic Neutron Spectrometers, WINS 2014}},
  issn         = {{2101-6275}},
  language     = {{eng}},
  series       = {{EPJ Web of Conferences}},
  title        = {{High-resolution neutron spectroscopy on protein solution samples}},
  url          = {{http://dx.doi.org/10.1051/epjconf/20158302005}},
  doi          = {{10.1051/epjconf/20158302005}},
  volume       = {{83}},
  year         = {{2015}},
}