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Capsule does not block antibody binding to PspA, a surface virulence protein of Streptococcus pneumoniae

Daniels, Calvin C; Briles, Travis C; Mirza, Shaper; Håkansson, Anders P LU and Briles, David E (2006) In Microbial Pathogenesis 40(5). p.33-228
Abstract

Of the proteins on the surface of Streptococcus pneumoniae, one of those best able to elicit protection against pneumococcal infection is pneumococcal surface protein A (PspA). Although this protein is attached to the membrane molecule, lipoteichoic acid, which is well beneath the capsule, PspA's ability to inhibit complement deposition and killing by apolactoferrin, suggests that it must have surface exposure. This study provides quantitative data showing that the capsular polysaccharide on types 2 and 3 pneumococci provides little or no masking ability of antibodies to bind PspA. Capsule was even observed to enhance, rather than inhibit the binding of two protective monoclonal antibodies to their epitopes on cell surface PspA. These... (More)

Of the proteins on the surface of Streptococcus pneumoniae, one of those best able to elicit protection against pneumococcal infection is pneumococcal surface protein A (PspA). Although this protein is attached to the membrane molecule, lipoteichoic acid, which is well beneath the capsule, PspA's ability to inhibit complement deposition and killing by apolactoferrin, suggests that it must have surface exposure. This study provides quantitative data showing that the capsular polysaccharide on types 2 and 3 pneumococci provides little or no masking ability of antibodies to bind PspA. Capsule was even observed to enhance, rather than inhibit the binding of two protective monoclonal antibodies to their epitopes on cell surface PspA. These results with antibodies to PspA are in contrast to binding by antibodies to the phosphocholine (PC) epitope of the lipoteichoic and teichoic acids. The binding of antibody to PC was largely, but not completely, blocked by capsular polysaccharide.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antibodies, Bacterial, Bacterial Capsules, Bacterial Proteins, Fluorescent Antibody Technique, Indirect, Mutation, Phosphorylcholine, Pneumococcal Infections, Streptococcus pneumoniae, Virulence
in
Microbial Pathogenesis
volume
40
issue
5
pages
6 pages
publisher
Academic Press
external identifiers
  • Scopus:33646251599
ISSN
0882-4010
DOI
10.1016/j.micpath.2006.01.007
language
English
LU publication?
no
id
b75378f8-5428-4899-a97f-2acf59c562bb
date added to LUP
2016-05-21 10:51:28
date last changed
2017-01-01 08:26:46
@article{b75378f8-5428-4899-a97f-2acf59c562bb,
  abstract     = {<p>Of the proteins on the surface of Streptococcus pneumoniae, one of those best able to elicit protection against pneumococcal infection is pneumococcal surface protein A (PspA). Although this protein is attached to the membrane molecule, lipoteichoic acid, which is well beneath the capsule, PspA's ability to inhibit complement deposition and killing by apolactoferrin, suggests that it must have surface exposure. This study provides quantitative data showing that the capsular polysaccharide on types 2 and 3 pneumococci provides little or no masking ability of antibodies to bind PspA. Capsule was even observed to enhance, rather than inhibit the binding of two protective monoclonal antibodies to their epitopes on cell surface PspA. These results with antibodies to PspA are in contrast to binding by antibodies to the phosphocholine (PC) epitope of the lipoteichoic and teichoic acids. The binding of antibody to PC was largely, but not completely, blocked by capsular polysaccharide.</p>},
  author       = {Daniels, Calvin C and Briles, Travis C and Mirza, Shaper and Håkansson, Anders P and Briles, David E},
  issn         = {0882-4010},
  keyword      = {Antibodies, Bacterial,Bacterial Capsules,Bacterial Proteins,Fluorescent Antibody Technique, Indirect,Mutation,Phosphorylcholine,Pneumococcal Infections,Streptococcus pneumoniae,Virulence},
  language     = {eng},
  number       = {5},
  pages        = {33--228},
  publisher    = {Academic Press},
  series       = {Microbial Pathogenesis},
  title        = {Capsule does not block antibody binding to PspA, a surface virulence protein of Streptococcus pneumoniae},
  url          = {http://dx.doi.org/10.1016/j.micpath.2006.01.007},
  volume       = {40},
  year         = {2006},
}