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Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae

Chao, Yashuan LU ; Bergenfelz, Caroline LU and Hakansson, Anders P LU (2019) In Methods in molecular biology (Clifton, N.J.) 1968. p.147-171
Abstract

It is estimated that over 80% of bacterial infections are associated with biofilm formation. Biofilms are organized bacterial communities formed on abiotic surfaces, such as implanted or inserted medical devices, or on biological surfaces, such as epithelial linings and mucosal surfaces. Biofilm growth is advantageous for the bacterial organism as it protects the bacteria from antimicrobial host factors and allows the bacteria to reside in the host without causing excessive inflammation. Like many other opportunistic pathogens of the respiratory tract, Streptococcus pneumoniae forms biofilms during asymptomatic carriage, which promotes, among other things, persistence in the niche, intraspecies and interspecies communication, and spread... (More)

It is estimated that over 80% of bacterial infections are associated with biofilm formation. Biofilms are organized bacterial communities formed on abiotic surfaces, such as implanted or inserted medical devices, or on biological surfaces, such as epithelial linings and mucosal surfaces. Biofilm growth is advantageous for the bacterial organism as it protects the bacteria from antimicrobial host factors and allows the bacteria to reside in the host without causing excessive inflammation. Like many other opportunistic pathogens of the respiratory tract, Streptococcus pneumoniae forms biofilms during asymptomatic carriage, which promotes, among other things, persistence in the niche, intraspecies and interspecies communication, and spread of bacterial DNA. Changes within the colonizing environment resulting from host assaults, such as virus infection, can induce biofilm dispersion where bacteria leave the biofilm and disseminate to other sites with ensuing infection. In this chapter, we present methodology to form complex biofilms in the nasopharynx of mice and to evaluate the biofilm structure and function in this environment. Furthermore, we present methods that recapitulate this biofilm phenotype in vitro by incorporating crucial factors associated with the host environment and describe how these models can be used to study biofilm function, transformation, and dispersion.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Streptococcus pneumoniae
series title
Methods in molecular biology (Clifton, N.J.)
volume
1968
pages
147 - 171
publisher
Humana Press
external identifiers
  • scopus:85063980293
ISSN
1940-6029
ISBN
978-1-4939-9198-3
978-1-4939-9199-0
DOI
10.1007/978-1-4939-9199-0_13
language
English
LU publication?
yes
id
b766f62b-9559-4929-9dd5-feaed2f80f45
date added to LUP
2019-04-05 15:53:02
date last changed
2019-05-14 04:53:22
@inbook{b766f62b-9559-4929-9dd5-feaed2f80f45,
  abstract     = {<p>It is estimated that over 80% of bacterial infections are associated with biofilm formation. Biofilms are organized bacterial communities formed on abiotic surfaces, such as implanted or inserted medical devices, or on biological surfaces, such as epithelial linings and mucosal surfaces. Biofilm growth is advantageous for the bacterial organism as it protects the bacteria from antimicrobial host factors and allows the bacteria to reside in the host without causing excessive inflammation. Like many other opportunistic pathogens of the respiratory tract, Streptococcus pneumoniae forms biofilms during asymptomatic carriage, which promotes, among other things, persistence in the niche, intraspecies and interspecies communication, and spread of bacterial DNA. Changes within the colonizing environment resulting from host assaults, such as virus infection, can induce biofilm dispersion where bacteria leave the biofilm and disseminate to other sites with ensuing infection. In this chapter, we present methodology to form complex biofilms in the nasopharynx of mice and to evaluate the biofilm structure and function in this environment. Furthermore, we present methods that recapitulate this biofilm phenotype in vitro by incorporating crucial factors associated with the host environment and describe how these models can be used to study biofilm function, transformation, and dispersion.</p>},
  author       = {Chao, Yashuan and Bergenfelz, Caroline and Hakansson, Anders P},
  isbn         = {978-1-4939-9198-3},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {147--171},
  publisher    = {Humana Press},
  series       = {Methods in molecular biology (Clifton, N.J.)},
  title        = {Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae},
  url          = {http://dx.doi.org/10.1007/978-1-4939-9199-0_13},
  volume       = {1968},
  year         = {2019},
}