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Cells of the nervous system

Rosenblad, Carl LU and Lundberg, Cecilia LU (2003) In Methods in Molecular Biology 229. p.299-307
Abstract
Direct in vivo gene transfer to the central nervous system (CNS) using recombinant lentiviral vectors (rLV) has emerged as a powerful technique to overexpress various genes of interest in different neuronal populations. This interest is exemplified by the increasing number of studies using rLV vectors to evaluate therapeutic proteins to correct disorders of the nervous system (1–3) or to explore a protein’s involvement in normal function or pathological processes (4). Compared to conventional trangenic techniques for overexpression, rLVs have several attractive features. For example, the level of transgene expression in cells transduced with a rLV is typically higher than obtained with knock-in techniques and is present already at 3–4 d... (More)
Direct in vivo gene transfer to the central nervous system (CNS) using recombinant lentiviral vectors (rLV) has emerged as a powerful technique to overexpress various genes of interest in different neuronal populations. This interest is exemplified by the increasing number of studies using rLV vectors to evaluate therapeutic proteins to correct disorders of the nervous system (1–3) or to explore a protein’s involvement in normal function or pathological processes (4). Compared to conventional trangenic techniques for overexpression, rLVs have several attractive features. For example, the level of transgene expression in cells transduced with a rLV is typically higher than obtained with knock-in techniques and is present already at 3–4 d after injection in vivo (5). Second, the expression may be directed to specific regions or cell populations depending on the anatomical location of the rLV injections as well as the design of the vector system. This may be advantageous for the study design since it allows unilateral overexpression, thereby creating an internal control in functional and morphological studies. Finally, it is considerably less time consuming than creating transgenic mouse lines. (Less)
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organization
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type
Contribution to journal
publication status
published
subject
keywords
Animals, Brain, Genetic Therapy, Genetic Vectors, Immunohistochemistry, Injections, Intraventricular, Lentivirus, Rats, Journal Article
in
Methods in Molecular Biology
volume
229
pages
9 pages
publisher
Springer
external identifiers
  • scopus:0141560356
ISSN
1064-3745
DOI
10.1385/1-59259-393-3:299
language
English
LU publication?
yes
id
b8b5afa5-980b-4601-8810-71630303e036
date added to LUP
2016-11-23 15:35:58
date last changed
2017-01-01 08:40:36
@article{b8b5afa5-980b-4601-8810-71630303e036,
  abstract     = {Direct in vivo gene transfer to the central nervous system (CNS) using recombinant lentiviral vectors (rLV) has emerged as a powerful technique to overexpress various genes of interest in different neuronal populations. This interest is exemplified by the increasing number of studies using rLV vectors to evaluate therapeutic proteins to correct disorders of the nervous system (1–3) or to explore a protein’s involvement in normal function or pathological processes (4). Compared to conventional trangenic techniques for overexpression, rLVs have several attractive features. For example, the level of transgene expression in cells transduced with a rLV is typically higher than obtained with knock-in techniques and is present already at 3–4 d after injection in vivo (5). Second, the expression may be directed to specific regions or cell populations depending on the anatomical location of the rLV injections as well as the design of the vector system. This may be advantageous for the study design since it allows unilateral overexpression, thereby creating an internal control in functional and morphological studies. Finally, it is considerably less time consuming than creating transgenic mouse lines. },
  author       = {Rosenblad, Carl and Lundberg, Cecilia},
  issn         = {1064-3745},
  keyword      = {Animals,Brain,Genetic Therapy,Genetic Vectors,Immunohistochemistry,Injections, Intraventricular,Lentivirus,Rats,Journal Article},
  language     = {eng},
  pages        = {299--307},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {Cells of the nervous system},
  url          = {http://dx.doi.org/10.1385/1-59259-393-3:299},
  volume       = {229},
  year         = {2003},
}