Are neuronal markers and neocortical graft-host interface influenced by housing conditions in rats with cortical infarct cavity?
(1999) In Brain Research Bulletin 48(2). p.165-171- Abstract
- The aim was to study if exposure to an enriched environment influenced graft-host interface and neuronal markers in neocortical grafts implanted in cortical infarct cavities 3 weeks after distal ligation of the middle cerebral artery in adult hypertensive rats. Half the rats were exposed to an enriched environment for 2 h daily 5 days a week starting 1 week after the arterial ligation. The brain was fixed by perfusion 4 weeks postgrafting. The immunoreactivity to glial fibrillary acidic protein, microtubule associated protein 2, and synaptophysin was studied in coronal paraffin-embedded sections. A distinct glial border separated the infarct cavity from the surrounding brain in sham-transplanted rats. Most grafts filled the larger part of... (More)
- The aim was to study if exposure to an enriched environment influenced graft-host interface and neuronal markers in neocortical grafts implanted in cortical infarct cavities 3 weeks after distal ligation of the middle cerebral artery in adult hypertensive rats. Half the rats were exposed to an enriched environment for 2 h daily 5 days a week starting 1 week after the arterial ligation. The brain was fixed by perfusion 4 weeks postgrafting. The immunoreactivity to glial fibrillary acidic protein, microtubule associated protein 2, and synaptophysin was studied in coronal paraffin-embedded sections. A distinct glial border separated the infarct cavity from the surrounding brain in sham-transplanted rats. Most grafts filled the larger part of the infarct cavity. In 8 of 18 transplants, 4 in each experimental group, part of the transplants protruded through the thin glial membrane that delineated the transplant-host interface into the adjacent host brain tissue. Microtubule associated protein 2 immunostained sections indicated bridging of dendrites in the host-transplant interface. Synaptophysin immunoreactivity was significantly higher in grafts than in contralateral cortex. However, graft morphology and neuronal marker immunoreactivity did not differ between rats housed in standard and activity stimulating cages. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1115192
- author
- Zeng, J ; Zhao, L R ; Nordborg, C ; Mattsson, Bengt LU and Johansson, Barbro LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cerebral infarction, GFAP, MAP2, Synaptophysin, Spontaneously hypertensive rats
- in
- Brain Research Bulletin
- volume
- 48
- issue
- 2
- pages
- 165 - 171
- publisher
- Elsevier
- external identifiers
-
- pmid:10230707
- scopus:0033555719
- ISSN
- 0361-9230
- DOI
- 10.1016/S0361-9230(98)00161-0
- language
- English
- LU publication?
- yes
- id
- b8b6469b-44ef-4b57-b761-3549e111e6ee (old id 1115192)
- date added to LUP
- 2016-04-01 12:23:43
- date last changed
- 2022-01-27 03:10:49
@article{b8b6469b-44ef-4b57-b761-3549e111e6ee,
abstract = {{The aim was to study if exposure to an enriched environment influenced graft-host interface and neuronal markers in neocortical grafts implanted in cortical infarct cavities 3 weeks after distal ligation of the middle cerebral artery in adult hypertensive rats. Half the rats were exposed to an enriched environment for 2 h daily 5 days a week starting 1 week after the arterial ligation. The brain was fixed by perfusion 4 weeks postgrafting. The immunoreactivity to glial fibrillary acidic protein, microtubule associated protein 2, and synaptophysin was studied in coronal paraffin-embedded sections. A distinct glial border separated the infarct cavity from the surrounding brain in sham-transplanted rats. Most grafts filled the larger part of the infarct cavity. In 8 of 18 transplants, 4 in each experimental group, part of the transplants protruded through the thin glial membrane that delineated the transplant-host interface into the adjacent host brain tissue. Microtubule associated protein 2 immunostained sections indicated bridging of dendrites in the host-transplant interface. Synaptophysin immunoreactivity was significantly higher in grafts than in contralateral cortex. However, graft morphology and neuronal marker immunoreactivity did not differ between rats housed in standard and activity stimulating cages.}},
author = {{Zeng, J and Zhao, L R and Nordborg, C and Mattsson, Bengt and Johansson, Barbro}},
issn = {{0361-9230}},
keywords = {{Cerebral infarction; GFAP; MAP2; Synaptophysin; Spontaneously hypertensive rats}},
language = {{eng}},
number = {{2}},
pages = {{165--171}},
publisher = {{Elsevier}},
series = {{Brain Research Bulletin}},
title = {{Are neuronal markers and neocortical graft-host interface influenced by housing conditions in rats with cortical infarct cavity?}},
url = {{http://dx.doi.org/10.1016/S0361-9230(98)00161-0}},
doi = {{10.1016/S0361-9230(98)00161-0}},
volume = {{48}},
year = {{1999}},
}