Adsorption and Activity of Trichoderma reesei Cellobiohydrolase I, Endoglucanase II, and the Corresponding Core Proteins on Steam Pretreated Willow
(1999) In Applied Biochemistry and Biotechnology 81(2). p.81-90- Abstract
- The adsorption and the hydrolytic action of purified cellulases of Trichoderma reesei, namely, cellobiohydrolase I (CBH I), endoglucanase II (EG II), and their core proteins, on steam-pretreated willow were compared. The two enzymes differed clearly in their adsorption and hydrolytic behavior. CBH I required the cellulose-binding domain (CBD) for efficient adsorption and hydrolysis, whereas EG II was able to adsorb to steam pretreated willow without its CBD. Absence of the CBD decreased the hydrolysis of cellulose by EG II, but the decrease was less pronounced than with CBH I. A linear relationship was observed between the amount of enzyme adsorbed and the degree of hydrolysis of cellulose only for CBH I. EG II and EG II core appeared to... (More)
- The adsorption and the hydrolytic action of purified cellulases of Trichoderma reesei, namely, cellobiohydrolase I (CBH I), endoglucanase II (EG II), and their core proteins, on steam-pretreated willow were compared. The two enzymes differed clearly in their adsorption and hydrolytic behavior. CBH I required the cellulose-binding domain (CBD) for efficient adsorption and hydrolysis, whereas EG II was able to adsorb to steam pretreated willow without its CBD. Absence of the CBD decreased the hydrolysis of cellulose by EG II, but the decrease was less pronounced than with CBH I. A linear relationship was observed between the amount of enzyme adsorbed and the degree of hydrolysis of cellulose only for CBH I. EG II and EG II core appeared to be able to hydrolyze only 1 to 2% of the substrate regardless of the amount of protein adsorbed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/125350
- author
- Kotiranta, Pia ; Karlsson, Johan ; Siika-Aho, Matti ; Medve, József ; Viikari, Liisa ; Tjerneld, Folke LU and Tenkanen, Maija
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adsorption, cellulose hydrolysis, cellobiohydrolase, endoglucanase, Trichoderma reesei
- in
- Applied Biochemistry and Biotechnology
- volume
- 81
- issue
- 2
- pages
- 81 - 90
- publisher
- Humana Press
- external identifiers
-
- scopus:0001958030
- ISSN
- 1559-0291
- language
- English
- LU publication?
- yes
- id
- b8b7b656-7632-4ddf-89bf-449de58192e6 (old id 125350)
- alternative location
- http://humanapress.com/index.php?option=com_journalshome&task=articledetails&category=journals&article_code=ABAB:81:2:81
- date added to LUP
- 2016-04-01 12:07:45
- date last changed
- 2022-01-26 23:14:03
@article{b8b7b656-7632-4ddf-89bf-449de58192e6, abstract = {{The adsorption and the hydrolytic action of purified cellulases of Trichoderma reesei, namely, cellobiohydrolase I (CBH I), endoglucanase II (EG II), and their core proteins, on steam-pretreated willow were compared. The two enzymes differed clearly in their adsorption and hydrolytic behavior. CBH I required the cellulose-binding domain (CBD) for efficient adsorption and hydrolysis, whereas EG II was able to adsorb to steam pretreated willow without its CBD. Absence of the CBD decreased the hydrolysis of cellulose by EG II, but the decrease was less pronounced than with CBH I. A linear relationship was observed between the amount of enzyme adsorbed and the degree of hydrolysis of cellulose only for CBH I. EG II and EG II core appeared to be able to hydrolyze only 1 to 2% of the substrate regardless of the amount of protein adsorbed.}}, author = {{Kotiranta, Pia and Karlsson, Johan and Siika-Aho, Matti and Medve, József and Viikari, Liisa and Tjerneld, Folke and Tenkanen, Maija}}, issn = {{1559-0291}}, keywords = {{Adsorption; cellulose hydrolysis; cellobiohydrolase; endoglucanase; Trichoderma reesei}}, language = {{eng}}, number = {{2}}, pages = {{81--90}}, publisher = {{Humana Press}}, series = {{Applied Biochemistry and Biotechnology}}, title = {{Adsorption and Activity of Trichoderma reesei Cellobiohydrolase I, Endoglucanase II, and the Corresponding Core Proteins on Steam Pretreated Willow}}, url = {{http://humanapress.com/index.php?option=com_journalshome&task=articledetails&category=journals&article_code=ABAB:81:2:81}}, volume = {{81}}, year = {{1999}}, }