Haemoglobin adducts and specific immunoglobulin G in humans as biomarkers of exposure to hexahydrophthalic anhydride
Jönsson, Bo A.G. LU ; Lindh, Christian H. LU
and Welinder, Hans
LU
(1997)
In Biomarkers
2(4).
p.239-246
- Abstract
The aim of this study was to determine whether haemoglobin adducts (Hb) of hexahydrophthalic anhydride (HHPA) and HHPA-specific immunoglobulin G (IgG) can be used as biomarkers of exposure to HHPA. The exposures of HHPA in 10 workers were determined from the mean urinary hexahydrophthalic acid (HHP acid) levels (range 76-3300 nmol HHP acid mmol-1 creatinine) during a period of 4 weeks. Blood was collected at the end of the period and Hb-HHPA adducts were analysed by gas chromatography mass spectrometry. The Hb-HHPA adduct levels ranged from 0.45 to 24.7 pmol g-1 Hb. There was a close correlation between the urinary HHP acid levels and the amount of Hb-HHPA adducts (r = 0.87). One-day exposures to HHPA and... (More)
The aim of this study was to determine whether haemoglobin adducts (Hb) of hexahydrophthalic anhydride (HHPA) and HHPA-specific immunoglobulin G (IgG) can be used as biomarkers of exposure to HHPA. The exposures of HHPA in 10 workers were determined from the mean urinary hexahydrophthalic acid (HHP acid) levels (range 76-3300 nmol HHP acid mmol-1 creatinine) during a period of 4 weeks. Blood was collected at the end of the period and Hb-HHPA adducts were analysed by gas chromatography mass spectrometry. The Hb-HHPA adduct levels ranged from 0.45 to 24.7 pmol g-1 Hb. There was a close correlation between the urinary HHP acid levels and the amount of Hb-HHPA adducts (r = 0.87). One-day exposures to HHPA and methylhexahydrophthalic anhydride (MHHPA) in 142 workers were determined from analysis of urinary HHP acid (range 0-3300 nmol HHP acid mmol-1 creatinine) and methylhexahydrophthalic acid (MHHP acid; range 0-1700 nmol MHHP acid mmol-1 creatinine). HHPA-specific IgG were analysed in the 142 workers with an ELISA method. The optical density for HHPA-specific IgG varied between 0 and 1.25. There was no statistically significant correlation between the sum of the urinary HHP acid and MHHP acid and the HHPA-specific IgG (r = 0.12; p = 0.14). Thus, Hb-HHPA adducts seem to be applicable as biomarkers of exposure to HHPA while the possible role of HHPA-specific IgG as an indicator of exposure has to be further evaluated.
(Less)
- author
- Jönsson, Bo A.G.
LU
; Lindh, Christian H.
LU
and Welinder, Hans
LU
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- keywords
- Biological monitoring, Haemoglobin adducts, Hexahydrophthalic anhydride, Methylhexahydrophthalic anhydride, Specific IgG
- in
- Biomarkers
- volume
- 2
- issue
- 4
- pages
- 239 - 246
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:0001420066
- ISSN
- 1354-750X
- DOI
- 10.1080/135475097231616
- language
- English
- LU publication?
- no
- id
- b8f40160-f8dc-4314-8163-81ae9df9ae3a
- date added to LUP
- 2025-11-28 11:19:22
- date last changed
- 2025-11-28 13:48:23
@article{b8f40160-f8dc-4314-8163-81ae9df9ae3a,
abstract = {{<p>The aim of this study was to determine whether haemoglobin adducts (Hb) of hexahydrophthalic anhydride (HHPA) and HHPA-specific immunoglobulin G (IgG) can be used as biomarkers of exposure to HHPA. The exposures of HHPA in 10 workers were determined from the mean urinary hexahydrophthalic acid (HHP acid) levels (range 76-3300 nmol HHP acid mmol<sup>-1</sup> creatinine) during a period of 4 weeks. Blood was collected at the end of the period and Hb-HHPA adducts were analysed by gas chromatography mass spectrometry. The Hb-HHPA adduct levels ranged from 0.45 to 24.7 pmol g<sup>-1</sup> Hb. There was a close correlation between the urinary HHP acid levels and the amount of Hb-HHPA adducts (r = 0.87). One-day exposures to HHPA and methylhexahydrophthalic anhydride (MHHPA) in 142 workers were determined from analysis of urinary HHP acid (range 0-3300 nmol HHP acid mmol<sup>-1</sup> creatinine) and methylhexahydrophthalic acid (MHHP acid; range 0-1700 nmol MHHP acid mmol<sup>-1</sup> creatinine). HHPA-specific IgG were analysed in the 142 workers with an ELISA method. The optical density for HHPA-specific IgG varied between 0 and 1.25. There was no statistically significant correlation between the sum of the urinary HHP acid and MHHP acid and the HHPA-specific IgG (r = 0.12; p = 0.14). Thus, Hb-HHPA adducts seem to be applicable as biomarkers of exposure to HHPA while the possible role of HHPA-specific IgG as an indicator of exposure has to be further evaluated.</p>}},
author = {{Jönsson, Bo A.G. and Lindh, Christian H. and Welinder, Hans}},
issn = {{1354-750X}},
keywords = {{Biological monitoring; Haemoglobin adducts; Hexahydrophthalic anhydride; Methylhexahydrophthalic anhydride; Specific IgG}},
language = {{eng}},
number = {{4}},
pages = {{239--246}},
publisher = {{Taylor & Francis}},
series = {{Biomarkers}},
title = {{Haemoglobin adducts and specific immunoglobulin G in humans as biomarkers of exposure to hexahydrophthalic anhydride}},
url = {{http://dx.doi.org/10.1080/135475097231616}},
doi = {{10.1080/135475097231616}},
volume = {{2}},
year = {{1997}},
}