N-cadherin is dispensable for pancreas development but required for beta-cell granule turnover.
(2010) In Genesis: The Journal of Genetics and Development 48(6). p.374-381- Abstract
- The cadherin family of cell adhesion molecules mediates adhesive interactions that are required for the formation and maintenance of tissues. Previously, we demonstrated that N-cadherin, which is required for numerous morphogenetic processes, is expressed in the pancreatic epithelium at E9.5, but later becomes restricted to endocrine aggregates in mice. To study the role of N-cadherin during pancreas formation and function we generated a tissue-specific knockout of N-cadherin in the early pancreatic epithelium by inter-crossing N-cadherin-floxed mice with Pdx1Cre mice. Analysis of pancreas-specific ablation of N-cadherin demonstrates that N-cadherin is dispensable for pancreatic development, but required for beta-cell granule turnover. The... (More)
- The cadherin family of cell adhesion molecules mediates adhesive interactions that are required for the formation and maintenance of tissues. Previously, we demonstrated that N-cadherin, which is required for numerous morphogenetic processes, is expressed in the pancreatic epithelium at E9.5, but later becomes restricted to endocrine aggregates in mice. To study the role of N-cadherin during pancreas formation and function we generated a tissue-specific knockout of N-cadherin in the early pancreatic epithelium by inter-crossing N-cadherin-floxed mice with Pdx1Cre mice. Analysis of pancreas-specific ablation of N-cadherin demonstrates that N-cadherin is dispensable for pancreatic development, but required for beta-cell granule turnover. The number of insulin secretory granules is significantly reduced in N-cadherin-deficient beta-cells, and as a consequence insulin secretion is decreased. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1626249
- author
- Johansson, Jenny LU ; Voss, Ulrikke LU ; Kesavan, Gokul LU ; Kostetskii, Igor ; Wierup, Nils LU ; Radice, Glenn L and Semb, Henrik LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Genesis: The Journal of Genetics and Development
- volume
- 48
- issue
- 6
- pages
- 374 - 381
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000279030800004
- pmid:20533404
- scopus:77953510208
- pmid:20533404
- ISSN
- 1526-954X
- DOI
- 10.1002/dvg.20628
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Stem Cell Center (013041110), Neuroendocrine Cell Biology (013212008)
- id
- b8f533ad-1eb4-4837-a5f3-488737a1bfb0 (old id 1626249)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/20533404?dopt=Abstract
- date added to LUP
- 2016-04-04 08:36:51
- date last changed
- 2022-08-23 04:23:52
@article{b8f533ad-1eb4-4837-a5f3-488737a1bfb0, abstract = {{The cadherin family of cell adhesion molecules mediates adhesive interactions that are required for the formation and maintenance of tissues. Previously, we demonstrated that N-cadherin, which is required for numerous morphogenetic processes, is expressed in the pancreatic epithelium at E9.5, but later becomes restricted to endocrine aggregates in mice. To study the role of N-cadherin during pancreas formation and function we generated a tissue-specific knockout of N-cadherin in the early pancreatic epithelium by inter-crossing N-cadherin-floxed mice with Pdx1Cre mice. Analysis of pancreas-specific ablation of N-cadherin demonstrates that N-cadherin is dispensable for pancreatic development, but required for beta-cell granule turnover. The number of insulin secretory granules is significantly reduced in N-cadherin-deficient beta-cells, and as a consequence insulin secretion is decreased.}}, author = {{Johansson, Jenny and Voss, Ulrikke and Kesavan, Gokul and Kostetskii, Igor and Wierup, Nils and Radice, Glenn L and Semb, Henrik}}, issn = {{1526-954X}}, language = {{eng}}, number = {{6}}, pages = {{374--381}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Genesis: The Journal of Genetics and Development}}, title = {{N-cadherin is dispensable for pancreas development but required for beta-cell granule turnover.}}, url = {{http://dx.doi.org/10.1002/dvg.20628}}, doi = {{10.1002/dvg.20628}}, volume = {{48}}, year = {{2010}}, }