Dietary Alpha-Ketoglutarate Partially Abolishes Adverse Changes in the Small Intestine after Gastric Bypass Surgery in a Rat Model
(2022) In Nutrients 14(10).- Abstract
Alpha-ketoglutarate (AKG) is one of the key metabolites that play a crucial role in cellular energy metabolism. Bariatric surgery is a life-saving procedure, but it carries many gastrointestinal side effects. The present study investigated the beneficial effects of dietary AKG on the structure, integrity, and absorption surface of the small intestine after bariatric surgery. Male 7-week-old Sprague Dowley rats underwent gastric bypass surgery, after which they received AKG, 0.2 g/kg body weight/day, administered in drinking water for 6 weeks. Changes in small intestinal morphol-ogy, including histomorphometric parameters of enteric plexuses, immunolocalization of claudin 3, MarvelD3, occludin and zonula ocludens 1 in the intestinal... (More)
Alpha-ketoglutarate (AKG) is one of the key metabolites that play a crucial role in cellular energy metabolism. Bariatric surgery is a life-saving procedure, but it carries many gastrointestinal side effects. The present study investigated the beneficial effects of dietary AKG on the structure, integrity, and absorption surface of the small intestine after bariatric surgery. Male 7-week-old Sprague Dowley rats underwent gastric bypass surgery, after which they received AKG, 0.2 g/kg body weight/day, administered in drinking water for 6 weeks. Changes in small intestinal morphol-ogy, including histomorphometric parameters of enteric plexuses, immunolocalization of claudin 3, MarvelD3, occludin and zonula ocludens 1 in the intestinal mucosa, and selected hormones, were evaluated. Proliferation, mucosal and submucosal thickness, number of intestinal villi and Paneth cells, and depth of crypts were increased; however, crypt activity, the absorption surface, the expression of claudin 3, MarvelD3, occludin and zonula ocludens 1 in the intestinal epithelium were decreased after gastric bypass surgery. Alpha-ketoglutarate supplementation partially improved intestinal structural parameters and epithelial integrity in rats undergoing this surgical procedure. Dietary AKG can abolish adverse functional changes in the intestinal mucosa, enteric nervous system, hormonal response, and maintenance of the intestinal barrier that occurred after gastric bypass surgery.
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- author
- Iwaniak, Paulina ; Tomaszewska, Ewa ; Muszyński, Siemowit ; Marszałek-Grabska, Marta ; Pierzynowski, Stefan Grzegorz LU and Dobrowolski, Piotr
- organization
- publishing date
- 2022-05-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- alpha-ketoglutaric acid, duodenum, gastric bypass, histomorphometry, jejunum
- in
- Nutrients
- volume
- 14
- issue
- 10
- article number
- 2062
- publisher
- MDPI AG
- external identifiers
-
- scopus:85129841920
- pmid:35631203
- ISSN
- 2072-6643
- DOI
- 10.3390/nu14102062
- language
- English
- LU publication?
- yes
- id
- b995c948-66a7-4b49-846c-00ee6ff51c7f
- date added to LUP
- 2022-12-08 11:33:07
- date last changed
- 2024-05-01 12:47:22
@article{b995c948-66a7-4b49-846c-00ee6ff51c7f,
abstract = {{<p>Alpha-ketoglutarate (AKG) is one of the key metabolites that play a crucial role in cellular energy metabolism. Bariatric surgery is a life-saving procedure, but it carries many gastrointestinal side effects. The present study investigated the beneficial effects of dietary AKG on the structure, integrity, and absorption surface of the small intestine after bariatric surgery. Male 7-week-old Sprague Dowley rats underwent gastric bypass surgery, after which they received AKG, 0.2 g/kg body weight/day, administered in drinking water for 6 weeks. Changes in small intestinal morphol-ogy, including histomorphometric parameters of enteric plexuses, immunolocalization of claudin 3, MarvelD3, occludin and zonula ocludens 1 in the intestinal mucosa, and selected hormones, were evaluated. Proliferation, mucosal and submucosal thickness, number of intestinal villi and Paneth cells, and depth of crypts were increased; however, crypt activity, the absorption surface, the expression of claudin 3, MarvelD3, occludin and zonula ocludens 1 in the intestinal epithelium were decreased after gastric bypass surgery. Alpha-ketoglutarate supplementation partially improved intestinal structural parameters and epithelial integrity in rats undergoing this surgical procedure. Dietary AKG can abolish adverse functional changes in the intestinal mucosa, enteric nervous system, hormonal response, and maintenance of the intestinal barrier that occurred after gastric bypass surgery.</p>}},
author = {{Iwaniak, Paulina and Tomaszewska, Ewa and Muszyński, Siemowit and Marszałek-Grabska, Marta and Pierzynowski, Stefan Grzegorz and Dobrowolski, Piotr}},
issn = {{2072-6643}},
keywords = {{alpha-ketoglutaric acid; duodenum; gastric bypass; histomorphometry; jejunum}},
language = {{eng}},
month = {{05}},
number = {{10}},
publisher = {{MDPI AG}},
series = {{Nutrients}},
title = {{Dietary Alpha-Ketoglutarate Partially Abolishes Adverse Changes in the Small Intestine after Gastric Bypass Surgery in a Rat Model}},
url = {{http://dx.doi.org/10.3390/nu14102062}},
doi = {{10.3390/nu14102062}},
volume = {{14}},
year = {{2022}},
}