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Comparative Kinetics of Supported Lipid Bilayer Formation on Silica Coated Vertically Oriented Highly Curved Nanowires and Planar Silica Surfaces

Valderas-Gutiérrez, Julia LU orcid ; Davtyan, Rubina LU ; Prinz, Christelle N. LU ; Sparr, Emma LU ; Jönsson, Peter LU orcid ; Linke, Heiner LU orcid and Höök, Fredrik LU (2025) In Nano Letters 25(8). p.3085-3092
Abstract

Supported lipid bilayers (SLBs), formed via lipid vesicle adsorption on highly curved silica surfaces, are widely used in biosensor applications and as models for curved cell membranes. However, SLB formation is often hindered on convex structures with radii comparable to the vesicles. In this study, lightguiding semiconductor nanowires (NWs), engineered for fluorescence signal enhancement, were used to compare the kinetics of SLB formation on vertically oriented NWs and planar silica surfaces. Time resolved fluorescence microscopy with single-molecule sensitivity revealed that while vesicle adsorption rates were similar on both surfaces lateral expansion of the SLB was up to three times faster on NWs than on the planar control. This... (More)

Supported lipid bilayers (SLBs), formed via lipid vesicle adsorption on highly curved silica surfaces, are widely used in biosensor applications and as models for curved cell membranes. However, SLB formation is often hindered on convex structures with radii comparable to the vesicles. In this study, lightguiding semiconductor nanowires (NWs), engineered for fluorescence signal enhancement, were used to compare the kinetics of SLB formation on vertically oriented NWs and planar silica surfaces. Time resolved fluorescence microscopy with single-molecule sensitivity revealed that while vesicle adsorption rates were similar on both surfaces lateral expansion of the SLB was up to three times faster on NWs than on the planar control. This accelerated expansion is attributed to lower energy penalties when SLBs spread along the cylindrical NWs compared with a planar surface, accompanied by accelerated SLB expansion driven by the merging of the SLB with excess lipids from vesicles accumulated on the NWs.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
epifluorescence microscopy, lightguiding, semiconductor nanowires, signal enhancement, supported lipid bilayer
in
Nano Letters
volume
25
issue
8
pages
8 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • scopus:85217193978
  • pmid:39914804
ISSN
1530-6984
DOI
10.1021/acs.nanolett.4c05303
language
English
LU publication?
yes
id
b9f4c856-e85d-445e-b3e3-8aec49dc8731
date added to LUP
2025-04-09 10:00:15
date last changed
2025-07-30 18:37:48
@article{b9f4c856-e85d-445e-b3e3-8aec49dc8731,
  abstract     = {{<p>Supported lipid bilayers (SLBs), formed via lipid vesicle adsorption on highly curved silica surfaces, are widely used in biosensor applications and as models for curved cell membranes. However, SLB formation is often hindered on convex structures with radii comparable to the vesicles. In this study, lightguiding semiconductor nanowires (NWs), engineered for fluorescence signal enhancement, were used to compare the kinetics of SLB formation on vertically oriented NWs and planar silica surfaces. Time resolved fluorescence microscopy with single-molecule sensitivity revealed that while vesicle adsorption rates were similar on both surfaces lateral expansion of the SLB was up to three times faster on NWs than on the planar control. This accelerated expansion is attributed to lower energy penalties when SLBs spread along the cylindrical NWs compared with a planar surface, accompanied by accelerated SLB expansion driven by the merging of the SLB with excess lipids from vesicles accumulated on the NWs.</p>}},
  author       = {{Valderas-Gutiérrez, Julia and Davtyan, Rubina and Prinz, Christelle N. and Sparr, Emma and Jönsson, Peter and Linke, Heiner and Höök, Fredrik}},
  issn         = {{1530-6984}},
  keywords     = {{epifluorescence microscopy; lightguiding; semiconductor nanowires; signal enhancement; supported lipid bilayer}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{8}},
  pages        = {{3085--3092}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Nano Letters}},
  title        = {{Comparative Kinetics of Supported Lipid Bilayer Formation on Silica Coated Vertically Oriented Highly Curved Nanowires and Planar Silica Surfaces}},
  url          = {{http://dx.doi.org/10.1021/acs.nanolett.4c05303}},
  doi          = {{10.1021/acs.nanolett.4c05303}},
  volume       = {{25}},
  year         = {{2025}},
}