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Experimental Evidence for a Cluster Glass Transition in Concentrated Lysozyme Solutions

Bergman, Maxime J. LU ; Garting, Tommy LU ; Schurtenberger, Peter LU and Stradner, Anna LU (2019) In Journal of Physical Chemistry B 123(10). p.2432-2438
Abstract


Lysozyme is known to form equilibrium clusters at pH ≈ 7.8 and at low ionic strength as a result of a mixed potential. While this cluster formation and the related dynamic and static structure factors have been extensively investigated, its consequences on the macroscopic dynamic behavior expressed by the zero shear viscosity η
0
remain controversial. Here we present results from a systematic investigation of η
0
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Lysozyme is known to form equilibrium clusters at pH ≈ 7.8 and at low ionic strength as a result of a mixed potential. While this cluster formation and the related dynamic and static structure factors have been extensively investigated, its consequences on the macroscopic dynamic behavior expressed by the zero shear viscosity η
0
remain controversial. Here we present results from a systematic investigation of η
0
using two complementary passive microrheology techniques, dynamic light scattering based tracer microrheology, and multiple particle tracking using confocal microscopy. The combination of these techniques with a simple but effective evaporation approach allows for reaching concentrations close to and above the arrest transition in a controlled and gentle way. We find a strong increase of η
0
with increasing volume fraction φ with an apparent divergence at φ ≈ 0.35, and unambiguously demonstrate that this is due to the existence of an arrest transition where a cluster glass forms. These findings demonstrate the power of tracer microrheology to investigate complex fluids, where weak temporary bonds and limited sample volumes make measurements with classical rheology challenging.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Physical Chemistry B
volume
123
issue
10
pages
2432 - 2438
publisher
The American Chemical Society
external identifiers
  • scopus:85062871765
ISSN
1520-6106
DOI
10.1021/acs.jpcb.8b11781
language
English
LU publication?
yes
id
b9f8a8b7-611a-4492-be9d-f4c4de6f15d7
date added to LUP
2019-03-21 14:05:07
date last changed
2019-08-14 04:34:10
@article{b9f8a8b7-611a-4492-be9d-f4c4de6f15d7,
  abstract     = {<p><br>
                                                         Lysozyme is known to form equilibrium clusters at pH ≈ 7.8 and at low ionic strength as a result of a mixed potential. While this cluster formation and the related dynamic and static structure factors have been extensively investigated, its consequences on the macroscopic dynamic behavior expressed by the zero shear viscosity η                             <br>
                            <sub>0</sub><br>
                                                          remain controversial. Here we present results from a systematic investigation of η                             <br>
                            <sub>0</sub><br>
                                                          using two complementary passive microrheology techniques, dynamic light scattering based tracer microrheology, and multiple particle tracking using confocal microscopy. The combination of these techniques with a simple but effective evaporation approach allows for reaching concentrations close to and above the arrest transition in a controlled and gentle way. We find a strong increase of η                             <br>
                            <sub>0</sub><br>
                                                          with increasing volume fraction φ with an apparent divergence at φ ≈ 0.35, and unambiguously demonstrate that this is due to the existence of an arrest transition where a cluster glass forms. These findings demonstrate the power of tracer microrheology to investigate complex fluids, where weak temporary bonds and limited sample volumes make measurements with classical rheology challenging.                         <br>
                        </p>},
  author       = {Bergman, Maxime J. and Garting, Tommy and Schurtenberger, Peter and Stradner, Anna},
  issn         = {1520-6106},
  language     = {eng},
  number       = {10},
  pages        = {2432--2438},
  publisher    = {The American Chemical Society},
  series       = {Journal of Physical Chemistry B},
  title        = {Experimental Evidence for a Cluster Glass Transition in Concentrated Lysozyme Solutions},
  url          = {http://dx.doi.org/10.1021/acs.jpcb.8b11781},
  volume       = {123},
  year         = {2019},
}