Toxicity screening of a gambierdiscus australes strain from the western mediterranean sea and identification of a novel maitotoxin analogue
(2021) In Marine Drugs 19(8).- Abstract
Dinoflagellate species of the genera Gambierdiscus and Fukuyoa are known to produce ciguatera poisoning-associated toxic compounds, such as ciguatoxins, or other toxins, such as maitotoxins. However, many species and strains remain poorly characterized in areas where they were recently identified, such as the western Mediterranean Sea. In previous studies carried out by our research group, a G. australes strain from the Balearic Islands (Mediterranean Sea) presenting MTX-like activity was characterized by LC-MS/MS and LC-HRMS detecting 44-methyl gambierone and gambieric acids C and D. However, MTX1, which is typically found in some G. australes strains from the Pacific Ocean, was not detected. Therefore, this study focuses on the... (More)
Dinoflagellate species of the genera Gambierdiscus and Fukuyoa are known to produce ciguatera poisoning-associated toxic compounds, such as ciguatoxins, or other toxins, such as maitotoxins. However, many species and strains remain poorly characterized in areas where they were recently identified, such as the western Mediterranean Sea. In previous studies carried out by our research group, a G. australes strain from the Balearic Islands (Mediterranean Sea) presenting MTX-like activity was characterized by LC-MS/MS and LC-HRMS detecting 44-methyl gambierone and gambieric acids C and D. However, MTX1, which is typically found in some G. australes strains from the Pacific Ocean, was not detected. Therefore, this study focuses on the identification of the compound responsible for the MTX-like toxicity in this strain. The G. australes strain was characterized not only using LC-MS instruments but also N2a-guided HPLC fractionation. Following this approach, several toxic compounds were identified in three fractions by LC-MS/MS and HRMS. A novel MTX analogue, named MTX5, was identified in the most toxic fraction, and 44-methyl gambierone and gambieric acids C and D contributed to the toxicity observed in other fractions of this strain. Thus, G. australes from the Mediterranean Sea produces MTX5 instead of MTX1 in contrast to some strains of the same species from the Pacific Ocean. No CTX precursors were detected, reinforcing the complexity of the identification of CTXs precursors in these regions.
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- author
- Estevez, Pablo ; Castro, David ; Leão-Martins, José Manuel ; Sibat, Manoëlla ; Tudó, Angels LU ; Dickey, Robert ; Diogene, Jorge ; Hess, Philipp and Gago-Martinez, Ana
- publishing date
- 2021-08
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ciguatera poisoning, Ciguatoxin, LC-HRMS, LC-MS/MS, Maitotoxin, N2a
- in
- Marine Drugs
- volume
- 19
- issue
- 8
- article number
- 460
- pages
- 19 pages
- publisher
- MDPI AG
- external identifiers
-
- scopus:85114383209
- pmid:34436299
- ISSN
- 1660-3397
- DOI
- 10.3390/MD19080460
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
- id
- bb277e6c-ce47-4b61-88d7-50386b70b282
- date added to LUP
- 2022-07-22 16:53:49
- date last changed
- 2024-06-13 18:02:59
@article{bb277e6c-ce47-4b61-88d7-50386b70b282,
abstract = {{<p>Dinoflagellate species of the genera Gambierdiscus and Fukuyoa are known to produce ciguatera poisoning-associated toxic compounds, such as ciguatoxins, or other toxins, such as maitotoxins. However, many species and strains remain poorly characterized in areas where they were recently identified, such as the western Mediterranean Sea. In previous studies carried out by our research group, a G. australes strain from the Balearic Islands (Mediterranean Sea) presenting MTX-like activity was characterized by LC-MS/MS and LC-HRMS detecting 44-methyl gambierone and gambieric acids C and D. However, MTX1, which is typically found in some G. australes strains from the Pacific Ocean, was not detected. Therefore, this study focuses on the identification of the compound responsible for the MTX-like toxicity in this strain. The G. australes strain was characterized not only using LC-MS instruments but also N2a-guided HPLC fractionation. Following this approach, several toxic compounds were identified in three fractions by LC-MS/MS and HRMS. A novel MTX analogue, named MTX5, was identified in the most toxic fraction, and 44-methyl gambierone and gambieric acids C and D contributed to the toxicity observed in other fractions of this strain. Thus, G. australes from the Mediterranean Sea produces MTX5 instead of MTX1 in contrast to some strains of the same species from the Pacific Ocean. No CTX precursors were detected, reinforcing the complexity of the identification of CTXs precursors in these regions.</p>}},
author = {{Estevez, Pablo and Castro, David and Leão-Martins, José Manuel and Sibat, Manoëlla and Tudó, Angels and Dickey, Robert and Diogene, Jorge and Hess, Philipp and Gago-Martinez, Ana}},
issn = {{1660-3397}},
keywords = {{Ciguatera poisoning; Ciguatoxin; LC-HRMS; LC-MS/MS; Maitotoxin; N2a}},
language = {{eng}},
number = {{8}},
publisher = {{MDPI AG}},
series = {{Marine Drugs}},
title = {{Toxicity screening of a <i>gambierdiscus australes</i> strain from the western mediterranean sea and identification of a novel maitotoxin analogue}},
url = {{http://dx.doi.org/10.3390/MD19080460}},
doi = {{10.3390/MD19080460}},
volume = {{19}},
year = {{2021}},
}