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Stretch To See - Lateral tension strongly determines cell survival in long-term cultures of adult porcine retina.

Taylor, Linnéa LU ; Moran, Damian LU ; Arnér, Karin LU ; Warrant, Eric LU and Ghosh, Fredrik LU (2013) In Investigative Ophthalmology & Visual Science 54(3). p.1845-1856
Abstract
PURPOSE: The purpose of this study is to explore the effect of lateral tension as a survival factor for retinal explants in vitro. The central nervous system (CNS) resides in a highly mechanical milieu. However, the importance of biomechanical homeostasis for normal CNS function has not been extensively explored. Diseases in which normal mechanical forces are disrupted, such as retinal detachment of the eye, are highly debilitating and the mechanisms underlying disease progression are not fully understood. METHODS: Using a porcine animal model, we developed a novel technique of culturing adult retinal explants under stretch for up to 10 days in vitro (DIV). These were compared to standard (no stretch) and free-floating cultured explants.... (More)
PURPOSE: The purpose of this study is to explore the effect of lateral tension as a survival factor for retinal explants in vitro. The central nervous system (CNS) resides in a highly mechanical milieu. However, the importance of biomechanical homeostasis for normal CNS function has not been extensively explored. Diseases in which normal mechanical forces are disrupted, such as retinal detachment of the eye, are highly debilitating and the mechanisms underlying disease progression are not fully understood. METHODS: Using a porcine animal model, we developed a novel technique of culturing adult retinal explants under stretch for up to 10 days in vitro (DIV). These were compared to standard (no stretch) and free-floating cultured explants. Cell survival was analysed using immunohistochemistry, and retinal architecture using hematoxylin and eosin staining. RESULTS: Compared to unstretched specimens, which at 10 DIV degenerate into a gliotic cell mass, stretched retinas display a profound preservation of the laminar retinal architecture as well as significantly increased neuronal cell survival, with no signs of impending gliosis. CONCLUSIONS: The results confirm that biomechanical tension is a vital factor in the maintenance of retinal tissue integrity, and suggest that mechanical cues are important components of pathological responses within the CNS. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Investigative Ophthalmology & Visual Science
volume
54
issue
3
pages
1845 - 1856
publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
external identifiers
  • wos:000316942400036
  • pmid:23422820
  • scopus:84875466658
  • pmid:23422820
ISSN
1552-5783
DOI
10.1167/iovs.12-11420
language
English
LU publication?
yes
id
bbb63637-a586-42f5-9214-cdcb9e98e3a2 (old id 3559592)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23422820?dopt=Abstract
date added to LUP
2016-04-01 10:06:19
date last changed
2020-01-05 04:42:39
@article{bbb63637-a586-42f5-9214-cdcb9e98e3a2,
  abstract     = {PURPOSE: The purpose of this study is to explore the effect of lateral tension as a survival factor for retinal explants in vitro. The central nervous system (CNS) resides in a highly mechanical milieu. However, the importance of biomechanical homeostasis for normal CNS function has not been extensively explored. Diseases in which normal mechanical forces are disrupted, such as retinal detachment of the eye, are highly debilitating and the mechanisms underlying disease progression are not fully understood. METHODS: Using a porcine animal model, we developed a novel technique of culturing adult retinal explants under stretch for up to 10 days in vitro (DIV). These were compared to standard (no stretch) and free-floating cultured explants. Cell survival was analysed using immunohistochemistry, and retinal architecture using hematoxylin and eosin staining. RESULTS: Compared to unstretched specimens, which at 10 DIV degenerate into a gliotic cell mass, stretched retinas display a profound preservation of the laminar retinal architecture as well as significantly increased neuronal cell survival, with no signs of impending gliosis. CONCLUSIONS: The results confirm that biomechanical tension is a vital factor in the maintenance of retinal tissue integrity, and suggest that mechanical cues are important components of pathological responses within the CNS.},
  author       = {Taylor, Linnéa and Moran, Damian and Arnér, Karin and Warrant, Eric and Ghosh, Fredrik},
  issn         = {1552-5783},
  language     = {eng},
  number       = {3},
  pages        = {1845--1856},
  publisher    = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
  series       = {Investigative Ophthalmology & Visual Science},
  title        = {Stretch To See - Lateral tension strongly determines cell survival in long-term cultures of adult porcine retina.},
  url          = {http://dx.doi.org/10.1167/iovs.12-11420},
  doi          = {10.1167/iovs.12-11420},
  volume       = {54},
  year         = {2013},
}