Advanced

Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone

Foubert, Astrid; Beloglazova, Natalia V.; Hedström, Martin LU and De Saeger, Sarah (2019) In Talanta 191. p.202-208
Abstract

A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent... (More)

A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL−1. It also demonstrated a good inter-assay precision (RSD < 10%). However, the tyramine-based capacitive immunosensor showed a bad repeatability (only 4 regeneration-interaction cycles were possible) and inter-assay precision (RSD > 15%) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Capacitive immunosensor, Mycotoxin, Polytyramine, Self-assembled monolayers, Zearalenone
in
Talanta
volume
191
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:85052501622
ISSN
0039-9140
DOI
10.1016/j.talanta.2018.08.062
language
English
LU publication?
yes
id
bbd0c87e-4dae-45ec-b8a6-d00041457cba
date added to LUP
2018-09-14 13:07:51
date last changed
2018-09-14 13:07:51
@article{bbd0c87e-4dae-45ec-b8a6-d00041457cba,
  abstract     = {<p>A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL<sup>−1</sup>. It also demonstrated a good inter-assay precision (RSD &lt; 10%). However, the tyramine-based capacitive immunosensor showed a bad repeatability (only 4 regeneration-interaction cycles were possible) and inter-assay precision (RSD &gt; 15%) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.</p>},
  author       = {Foubert, Astrid and Beloglazova, Natalia V. and Hedström, Martin and De Saeger, Sarah},
  issn         = {0039-9140},
  keyword      = {Capacitive immunosensor,Mycotoxin,Polytyramine,Self-assembled monolayers,Zearalenone},
  language     = {eng},
  month        = {01},
  pages        = {202--208},
  publisher    = {Elsevier},
  series       = {Talanta},
  title        = {Antibody immobilization strategy for the development of a capacitive immunosensor detecting zearalenone},
  url          = {http://dx.doi.org/10.1016/j.talanta.2018.08.062},
  volume       = {191},
  year         = {2019},
}