Advanced

Calpain inhibitors delay injury-induced apoptosis in adult mouse spinal cord motor neurons

Momeni, HamidReza LU and Kanje, Martin LU (2006) In NeuroReport 17(8). p.761-765
Abstract
Here, we investigated the effect of calpain inhibitors on apoptosis in organotypic adult spinal cord slices from mice. An increase in calpain I immunoreactivity was found in the nuclei of motor neurons from slices cultured for 30 min. After 4 h, the immunopositive motor neurons exhibited apoptotic changes including nuclear and chromatin condensation. Eight hours after excision, most motor neurons showed nuclear apoptotic features. Two calpain inhibitors, leupeptin and calpain inhibitor XI, inhibited apoptosis in the motor neurons while the caspase inhibitor Z-VAD.fmk had no effect. Leupeptin, but not calpain inhibitor XI and Z-VAD.fmk, also inhibited nucleosomal DNA fragmentation. These results suggest the involvement of calpain I in the... (More)
Here, we investigated the effect of calpain inhibitors on apoptosis in organotypic adult spinal cord slices from mice. An increase in calpain I immunoreactivity was found in the nuclei of motor neurons from slices cultured for 30 min. After 4 h, the immunopositive motor neurons exhibited apoptotic changes including nuclear and chromatin condensation. Eight hours after excision, most motor neurons showed nuclear apoptotic features. Two calpain inhibitors, leupeptin and calpain inhibitor XI, inhibited apoptosis in the motor neurons while the caspase inhibitor Z-VAD.fmk had no effect. Leupeptin, but not calpain inhibitor XI and Z-VAD.fmk, also inhibited nucleosomal DNA fragmentation. These results suggest the involvement of calpain I in the induction of apoptosis in motor neurons of adult spinal cord and that apoptosis can be triggered independent of caspase activation. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
spinal cord, culture, organ, motor neurons, caspase, calpain inhibitor, apoptosis, calpain
in
NeuroReport
volume
17
issue
8
pages
761 - 765
publisher
Lippincott Williams & Wilkins
external identifiers
  • pmid:16708011
  • wos:000238142300001
  • scopus:33744492320
ISSN
1473-558X
language
English
LU publication?
yes
id
bc56b5fe-b03a-42d9-a893-bb377ce4afbb (old id 406756)
alternative location
http://www.neuroreport.com/pt/re/neuroreport/abstract.00001756-200605290-00001.htm;jsessionid=HNnDgGW80stxSv6rnrXx0q83KRRsLv3rL5sGp0n21JGtyTCJmwLJ!-1323538283!181195628!8091!-1
date added to LUP
2007-10-10 21:58:16
date last changed
2019-08-07 01:51:50
@article{bc56b5fe-b03a-42d9-a893-bb377ce4afbb,
  abstract     = {Here, we investigated the effect of calpain inhibitors on apoptosis in organotypic adult spinal cord slices from mice. An increase in calpain I immunoreactivity was found in the nuclei of motor neurons from slices cultured for 30 min. After 4 h, the immunopositive motor neurons exhibited apoptotic changes including nuclear and chromatin condensation. Eight hours after excision, most motor neurons showed nuclear apoptotic features. Two calpain inhibitors, leupeptin and calpain inhibitor XI, inhibited apoptosis in the motor neurons while the caspase inhibitor Z-VAD.fmk had no effect. Leupeptin, but not calpain inhibitor XI and Z-VAD.fmk, also inhibited nucleosomal DNA fragmentation. These results suggest the involvement of calpain I in the induction of apoptosis in motor neurons of adult spinal cord and that apoptosis can be triggered independent of caspase activation.},
  author       = {Momeni, HamidReza and Kanje, Martin},
  issn         = {1473-558X},
  keyword      = {spinal cord,culture,organ,motor neurons,caspase,calpain inhibitor,apoptosis,calpain},
  language     = {eng},
  number       = {8},
  pages        = {761--765},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {NeuroReport},
  title        = {Calpain inhibitors delay injury-induced apoptosis in adult mouse spinal cord motor neurons},
  volume       = {17},
  year         = {2006},
}