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Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

Trieu, Anthony T. ; Burleigh, Stephen H. LU ; Kardailsky, Igor V. ; Maldonado-Mendoza, Ignacio E. ; Versaw, Wayne K. ; Blaylock, Laura A. ; Shin, Heungsop ; Chiou, Tzyy Jen ; Katagi, Hiroaki and Dewbre, Gary R. , et al. (2000) In Plant Journal 22(6). p.531-541
Abstract


Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants... (More)


Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T
2
generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.

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publishing date
type
Contribution to journal
publication status
published
in
Plant Journal
volume
22
issue
6
pages
11 pages
publisher
Wiley-Blackwell
external identifiers
  • pmid:10886773
  • scopus:0033919939
ISSN
0960-7412
DOI
10.1046/j.1365-313X.2000.00757.x
language
English
LU publication?
no
id
bc85d7a2-8d47-4592-9c08-452bdaa01d38
date added to LUP
2019-03-08 16:00:50
date last changed
2020-08-05 05:11:51
@article{bc85d7a2-8d47-4592-9c08-452bdaa01d38,
  abstract     = {<p><br>
                            Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T<br>
                            <sub>2</sub><br>
                             generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.<br>
                        </p>},
  author       = {Trieu, Anthony T. and Burleigh, Stephen H. and Kardailsky, Igor V. and Maldonado-Mendoza, Ignacio E. and Versaw, Wayne K. and Blaylock, Laura A. and Shin, Heungsop and Chiou, Tzyy Jen and Katagi, Hiroaki and Dewbre, Gary R. and Weigel, Detlef and Harrison, Maria J.},
  issn         = {0960-7412},
  language     = {eng},
  month        = {06},
  number       = {6},
  pages        = {531--541},
  publisher    = {Wiley-Blackwell},
  series       = {Plant Journal},
  title        = {Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.2000.00757.x},
  doi          = {10.1046/j.1365-313X.2000.00757.x},
  volume       = {22},
  year         = {2000},
}