Photoconjugation of Molecularly Imprinted Polymer Nanoparticles for Surface-Enhanced Raman Detection of Propranolol
Kamra, Tripta LU ; Xu, Changgang LU ; Montelius, Lars LU ; Schnadt, Joachim LU
; Wijesundera, Samurdhi A.
; Yan, Mingdi
and Ye, Lei
LU
(2015)
In ACS Applied Materials and Interfaces
7(49).
p.27479-27485
- Abstract
- We report a simple and versatile method to covalently immobilize molecularly imprinted polymer (MIP) nanoparticles on a Raman active substrate (Klarite) using a disulfide-derivatized perfluorophenylazide (PFPA-disulfide). Gold-coated Klarite was functionalized with PFPA-disulfide via a gold sulfur bond. Upon light radiation, the available azido groups were converted to highly reactive singlet perfluorophenyl nitrene that undergoes a CH insertion reaction and form covalent bonds with the MIP nanoparticles. The resulting surfaces were characterized using scanning electron microscopy and surface enhanced Raman spectroscopy to study the morphology and template affinity of the surfaces, respectively. The Raman measurements clearly show a... (More)
- We report a simple and versatile method to covalently immobilize molecularly imprinted polymer (MIP) nanoparticles on a Raman active substrate (Klarite) using a disulfide-derivatized perfluorophenylazide (PFPA-disulfide). Gold-coated Klarite was functionalized with PFPA-disulfide via a gold sulfur bond. Upon light radiation, the available azido groups were converted to highly reactive singlet perfluorophenyl nitrene that undergoes a CH insertion reaction and form covalent bonds with the MIP nanoparticles. The resulting surfaces were characterized using scanning electron microscopy and surface enhanced Raman spectroscopy to study the morphology and template affinity of the surfaces, respectively. The Raman measurements clearly show a dose-responsive signal when propranolol binds to the MIP surface. Because the MIP particles were covalently attached to the Raman active substrate, the sensing surface was stable and could be reused after regeneration in acetic acid solution. The MIP-based Raman sensor was used successfully to detect propranolol in urine samples (7.7 X 10(-4) M). Our results show that the high selectivity of MLPs and the fingerprint Raman identification can be integrated into a compact sensing unit using high-efficiency photoconjugation. Thus, the method proposed is reliable, efficient and fast for fabricating label-free chemical sensors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8548816
- author
- Kamra, Tripta
LU
; Xu, Changgang
LU
; Montelius, Lars
LU
; Schnadt, Joachim
LU
; Wijesundera, Samurdhi A.
; Yan, Mingdi
and Ye, Lei
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- molecularly imprinted polymers, propranolol, perfluorophenylazide, disulfide, photocoupling, Klarite, surface-enhanced Raman spectroscopy, urine
- in
- ACS Applied Materials and Interfaces
- volume
- 7
- issue
- 49
- pages
- 27479 - 27485
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000366873900054
- scopus:84950269220
- pmid:26595262
- ISSN
- 1944-8244
- DOI
- 10.1021/acsami.5b09500
- language
- English
- LU publication?
- yes
- id
- bca76f86-115d-46a8-82d8-c4e3a7a9fe98 (old id 8548816)
- date added to LUP
- 2016-04-01 10:22:21
- date last changed
- 2023-11-09 19:16:03
@article{bca76f86-115d-46a8-82d8-c4e3a7a9fe98,
abstract = {{We report a simple and versatile method to covalently immobilize molecularly imprinted polymer (MIP) nanoparticles on a Raman active substrate (Klarite) using a disulfide-derivatized perfluorophenylazide (PFPA-disulfide). Gold-coated Klarite was functionalized with PFPA-disulfide via a gold sulfur bond. Upon light radiation, the available azido groups were converted to highly reactive singlet perfluorophenyl nitrene that undergoes a CH insertion reaction and form covalent bonds with the MIP nanoparticles. The resulting surfaces were characterized using scanning electron microscopy and surface enhanced Raman spectroscopy to study the morphology and template affinity of the surfaces, respectively. The Raman measurements clearly show a dose-responsive signal when propranolol binds to the MIP surface. Because the MIP particles were covalently attached to the Raman active substrate, the sensing surface was stable and could be reused after regeneration in acetic acid solution. The MIP-based Raman sensor was used successfully to detect propranolol in urine samples (7.7 X 10(-4) M). Our results show that the high selectivity of MLPs and the fingerprint Raman identification can be integrated into a compact sensing unit using high-efficiency photoconjugation. Thus, the method proposed is reliable, efficient and fast for fabricating label-free chemical sensors.}},
author = {{Kamra, Tripta and Xu, Changgang and Montelius, Lars and Schnadt, Joachim and Wijesundera, Samurdhi A. and Yan, Mingdi and Ye, Lei}},
issn = {{1944-8244}},
keywords = {{molecularly imprinted polymers; propranolol; perfluorophenylazide; disulfide; photocoupling; Klarite; surface-enhanced Raman spectroscopy; urine}},
language = {{eng}},
number = {{49}},
pages = {{27479--27485}},
publisher = {{The American Chemical Society (ACS)}},
series = {{ACS Applied Materials and Interfaces}},
title = {{Photoconjugation of Molecularly Imprinted Polymer Nanoparticles for Surface-Enhanced Raman Detection of Propranolol}},
url = {{http://dx.doi.org/10.1021/acsami.5b09500}},
doi = {{10.1021/acsami.5b09500}},
volume = {{7}},
year = {{2015}},
}