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Modeling ebola virus genome replication and transcription with minigenome systems

Cressey, Tessa; Brauburger, Kristina LU and Mühlberger, Elke (2017) In Methods in Molecular Biology 1628. p.79-92
Abstract

In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Ebola virus, Filoviruses, Firefly luciferase assay, Minigenome, Replication, Replication promoter, Reporter gene, Transcription, Zaire ebolavirus, β-galactosidase enzyme assay
in
Methods in Molecular Biology
editor
Hoenen, Thomas; Groseth, Allison; and
volume
1628
pages
14 pages
publisher
Humana Press
external identifiers
  • scopus:85020175520
ISSN
10643745
DOI
10.1007/978-1-4939-7116-9_6
language
English
LU publication?
yes
id
bd2d7005-ee1f-4f18-9006-e063ca7d2dc4
date added to LUP
2017-06-30 09:49:58
date last changed
2017-06-30 09:49:58
@inbook{bd2d7005-ee1f-4f18-9006-e063ca7d2dc4,
  abstract     = {<p>In this chapter, we describe the minigenome system for Ebola virus (EBOV), which reconstitutes EBOV polymerase activity in cells and can be used to model viral genome replication and transcription. This protocol comprises all steps including cell culture, plasmid preparation, transfection, and luciferase reporter assay readout.</p>},
  author       = {Cressey, Tessa and Brauburger, Kristina and Mühlberger, Elke},
  editor       = {Hoenen, Thomas and Groseth, Allison},
  issn         = {10643745},
  keyword      = {Ebola virus,Filoviruses,Firefly luciferase assay,Minigenome,Replication,Replication promoter,Reporter gene,Transcription,Zaire ebolavirus,β-galactosidase enzyme assay},
  language     = {eng},
  pages        = {79--92},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Modeling ebola virus genome replication and transcription with minigenome systems},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7116-9_6},
  volume       = {1628},
  year         = {2017},
}