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Ethanol and erythrocyte membrane interaction: a hemorheologic perspective.

Mesquita, Rui ; Goncalves, Isabel LU orcid ; Dias, S ; Sargento, Luís ; Saldanha, C and Martins e Silva, J (1999) In Clinical Hemorheology and Microcirculation 21(2). p.95-98
Abstract
Previous studies have documented structural and functional changes induced by ethanol-erythrocyte membrane interaction. In order to perform an in vitro study on the effect of different ethanol concentrations on erythrocyte hemorheologic properties, blood samples were collected from 21 male donors at the Hospital of Santa Maria. Whole blood aliquots were incubated with ethanol solutions of rising concentrations. The following parameters were measured: erythrocyte aggregation, haemoglobin, carboxyhaemoglobin and methaemoglobin concentrations, hematocrit, plasma osmolality and erythrocyte membrane fluidity (fluorescence polarisation probes TMA-DPH and DPH). With ethanol blood concentrations of 45 mM a rise in plasma osmolality (0.352 Osm/kg... (More)
Previous studies have documented structural and functional changes induced by ethanol-erythrocyte membrane interaction. In order to perform an in vitro study on the effect of different ethanol concentrations on erythrocyte hemorheologic properties, blood samples were collected from 21 male donors at the Hospital of Santa Maria. Whole blood aliquots were incubated with ethanol solutions of rising concentrations. The following parameters were measured: erythrocyte aggregation, haemoglobin, carboxyhaemoglobin and methaemoglobin concentrations, hematocrit, plasma osmolality and erythrocyte membrane fluidity (fluorescence polarisation probes TMA-DPH and DPH). With ethanol blood concentrations of 45 mM a rise in plasma osmolality (0.352 Osm/kg H2O vs 0.310 Osm/kg H2O; p < 0.001) was verified. With 67 mM concentration a decrease of erythrocyte aggregation (11.03 vs 12.81; p < 0.05) and an increase in plasma osmolality (0.380 Osm/kg H2O vs 0.310 Osm/kg H2O; p < 0.001) were obtained. In conclusion, ethanol only changes erythrocyte aggregation for a concentration of 67 mM. These data could lead to future changes in therapeutic approaches to situations such as alcoholic coma. (Less)
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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical Hemorheology and Microcirculation
volume
21
issue
2
pages
95 - 98
publisher
IOS Press
external identifiers
  • scopus:0032755940
ISSN
1386-0291
language
English
LU publication?
no
id
bd483843-e11f-46e1-aa4a-995814419fd8
alternative location
https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch175
date added to LUP
2019-06-14 12:34:42
date last changed
2022-01-31 21:52:07
@article{bd483843-e11f-46e1-aa4a-995814419fd8,
  abstract     = {{Previous studies have documented structural and functional changes induced by ethanol-erythrocyte membrane interaction. In order to perform an in vitro study on the effect of different ethanol concentrations on erythrocyte hemorheologic properties, blood samples were collected from 21 male donors at the Hospital of Santa Maria. Whole blood aliquots were incubated with ethanol solutions of rising concentrations. The following parameters were measured: erythrocyte aggregation, haemoglobin, carboxyhaemoglobin and methaemoglobin concentrations, hematocrit, plasma osmolality and erythrocyte membrane fluidity (fluorescence polarisation probes TMA-DPH and DPH). With ethanol blood concentrations of 45 mM a rise in plasma osmolality (0.352 Osm/kg H2O vs 0.310 Osm/kg H2O; p &lt; 0.001) was verified. With 67 mM concentration a decrease of erythrocyte aggregation (11.03 vs 12.81; p &lt; 0.05) and an increase in plasma osmolality (0.380 Osm/kg H2O vs 0.310 Osm/kg H2O; p &lt; 0.001) were obtained. In conclusion, ethanol only changes erythrocyte aggregation for a concentration of 67 mM. These data could lead to future changes in therapeutic approaches to situations such as alcoholic coma.}},
  author       = {{Mesquita, Rui and Goncalves, Isabel and Dias, S and Sargento, Luís and Saldanha, C and Martins e Silva, J}},
  issn         = {{1386-0291}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{95--98}},
  publisher    = {{IOS Press}},
  series       = {{Clinical Hemorheology and Microcirculation}},
  title        = {{Ethanol and erythrocyte membrane interaction: a hemorheologic perspective.}},
  url          = {{https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch175}},
  volume       = {{21}},
  year         = {{1999}},
}