Ethanol and erythrocyte membrane interaction: a hemorheologic perspective.
(1999) In Clinical Hemorheology and Microcirculation 21(2). p.95-98- Abstract
- Previous studies have documented structural and functional changes induced by ethanol-erythrocyte membrane interaction. In order to perform an in vitro study on the effect of different ethanol concentrations on erythrocyte hemorheologic properties, blood samples were collected from 21 male donors at the Hospital of Santa Maria. Whole blood aliquots were incubated with ethanol solutions of rising concentrations. The following parameters were measured: erythrocyte aggregation, haemoglobin, carboxyhaemoglobin and methaemoglobin concentrations, hematocrit, plasma osmolality and erythrocyte membrane fluidity (fluorescence polarisation probes TMA-DPH and DPH). With ethanol blood concentrations of 45 mM a rise in plasma osmolality (0.352 Osm/kg... (More)
- Previous studies have documented structural and functional changes induced by ethanol-erythrocyte membrane interaction. In order to perform an in vitro study on the effect of different ethanol concentrations on erythrocyte hemorheologic properties, blood samples were collected from 21 male donors at the Hospital of Santa Maria. Whole blood aliquots were incubated with ethanol solutions of rising concentrations. The following parameters were measured: erythrocyte aggregation, haemoglobin, carboxyhaemoglobin and methaemoglobin concentrations, hematocrit, plasma osmolality and erythrocyte membrane fluidity (fluorescence polarisation probes TMA-DPH and DPH). With ethanol blood concentrations of 45 mM a rise in plasma osmolality (0.352 Osm/kg H2O vs 0.310 Osm/kg H2O; p < 0.001) was verified. With 67 mM concentration a decrease of erythrocyte aggregation (11.03 vs 12.81; p < 0.05) and an increase in plasma osmolality (0.380 Osm/kg H2O vs 0.310 Osm/kg H2O; p < 0.001) were obtained. In conclusion, ethanol only changes erythrocyte aggregation for a concentration of 67 mM. These data could lead to future changes in therapeutic approaches to situations such as alcoholic coma. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/bd483843-e11f-46e1-aa4a-995814419fd8
- author
- Mesquita, Rui ; Goncalves, Isabel LU ; Dias, S ; Sargento, Luís ; Saldanha, C and Martins e Silva, J
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Clinical Hemorheology and Microcirculation
- volume
- 21
- issue
- 2
- pages
- 95 - 98
- publisher
- IOS Press
- external identifiers
-
- scopus:0032755940
- ISSN
- 1386-0291
- language
- English
- LU publication?
- no
- id
- bd483843-e11f-46e1-aa4a-995814419fd8
- alternative location
- https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch175
- date added to LUP
- 2019-06-14 12:34:42
- date last changed
- 2022-01-31 21:52:07
@article{bd483843-e11f-46e1-aa4a-995814419fd8, abstract = {{Previous studies have documented structural and functional changes induced by ethanol-erythrocyte membrane interaction. In order to perform an in vitro study on the effect of different ethanol concentrations on erythrocyte hemorheologic properties, blood samples were collected from 21 male donors at the Hospital of Santa Maria. Whole blood aliquots were incubated with ethanol solutions of rising concentrations. The following parameters were measured: erythrocyte aggregation, haemoglobin, carboxyhaemoglobin and methaemoglobin concentrations, hematocrit, plasma osmolality and erythrocyte membrane fluidity (fluorescence polarisation probes TMA-DPH and DPH). With ethanol blood concentrations of 45 mM a rise in plasma osmolality (0.352 Osm/kg H2O vs 0.310 Osm/kg H2O; p < 0.001) was verified. With 67 mM concentration a decrease of erythrocyte aggregation (11.03 vs 12.81; p < 0.05) and an increase in plasma osmolality (0.380 Osm/kg H2O vs 0.310 Osm/kg H2O; p < 0.001) were obtained. In conclusion, ethanol only changes erythrocyte aggregation for a concentration of 67 mM. These data could lead to future changes in therapeutic approaches to situations such as alcoholic coma.}}, author = {{Mesquita, Rui and Goncalves, Isabel and Dias, S and Sargento, Luís and Saldanha, C and Martins e Silva, J}}, issn = {{1386-0291}}, language = {{eng}}, number = {{2}}, pages = {{95--98}}, publisher = {{IOS Press}}, series = {{Clinical Hemorheology and Microcirculation}}, title = {{Ethanol and erythrocyte membrane interaction: a hemorheologic perspective.}}, url = {{https://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch175}}, volume = {{21}}, year = {{1999}}, }