Characterization of galactoglucomannan extracted from spruce (Picea abies) by heat-fractionation at different conditions
(2003) In Carbohydrate Polymers 51(2). p.203-211- Abstract
- Water-soluble hemicelluloses were extracted from spruce chips by microwave heat-fractionation. The galactoglucomannan (GGM) extraction was evaluated on the basis of weight-average molecular weight (MW), yield and carbohydrate composition of the GGM. The MW was determined by size-exclusion chromatography with column calibration using off-line MALDI-MS analysis, and determination of mannan content in the fractions collected. Water impregnated spruce chips were heat-fractionated at three different temperatures (180, 190, and 200oC). The spruce chips were also impregnated in NaOH solutions of different concentrations, and then heat-fractionated at 190oC for 5min. The highest mannan yield (78% based on the amount in the raw material) was... (More)
- Water-soluble hemicelluloses were extracted from spruce chips by microwave heat-fractionation. The galactoglucomannan (GGM) extraction was evaluated on the basis of weight-average molecular weight (MW), yield and carbohydrate composition of the GGM. The MW was determined by size-exclusion chromatography with column calibration using off-line MALDI-MS analysis, and determination of mannan content in the fractions collected. Water impregnated spruce chips were heat-fractionated at three different temperatures (180, 190, and 200oC). The spruce chips were also impregnated in NaOH solutions of different concentrations, and then heat-fractionated at 190oC for 5min. The highest mannan yield (78% based on the amount in the raw material) was obtained from water impregnated spruce chips heat-fractionated at 190oC for 5min (MW of 3800). The highest MW (14,000) was obtained from impregnation with 2% NaOH (190oC, 5min), but the yield of mannan was very low (3%). Impregnation with 0.025% NaOH and heat-fractionation at 190oC for 5min resulted in extraction of GGM with MW of 9500 and a mannan yield of 31%. When the spruce chips were impregnated with =<0.05% NaOH an O-acetyl-galactoglucomannan was extracted, whereas when higher NaOH charges were used in the impregnation, the extracted GGM lacked acetyl groups. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/124689
- author
- Lundqvist, Jon LU ; Jacobs, Anna ; Palm, Magnus LU ; Zacchi, Guido LU ; Dahlman, Olof and Stålbrand, Henrik LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Spruce (Picea abies), Hemicellulose, O-acetyl-galactoglucomannan, Microwave oven, Size-exclusion chromatography, MALDI-MS
- in
- Carbohydrate Polymers
- volume
- 51
- issue
- 2
- pages
- 203 - 211
- publisher
- Elsevier
- external identifiers
-
- wos:000178715500009
- scopus:0037289902
- ISSN
- 0144-8617
- DOI
- 10.1016/S0144-8617(02)00111-X
- language
- English
- LU publication?
- yes
- id
- bd52b908-c2d1-4e20-9b5e-688e2595acfa (old id 124689)
- date added to LUP
- 2016-04-01 12:27:38
- date last changed
- 2023-11-12 01:45:03
@article{bd52b908-c2d1-4e20-9b5e-688e2595acfa, abstract = {{Water-soluble hemicelluloses were extracted from spruce chips by microwave heat-fractionation. The galactoglucomannan (GGM) extraction was evaluated on the basis of weight-average molecular weight (MW), yield and carbohydrate composition of the GGM. The MW was determined by size-exclusion chromatography with column calibration using off-line MALDI-MS analysis, and determination of mannan content in the fractions collected. Water impregnated spruce chips were heat-fractionated at three different temperatures (180, 190, and 200oC). The spruce chips were also impregnated in NaOH solutions of different concentrations, and then heat-fractionated at 190oC for 5min. The highest mannan yield (78% based on the amount in the raw material) was obtained from water impregnated spruce chips heat-fractionated at 190oC for 5min (MW of 3800). The highest MW (14,000) was obtained from impregnation with 2% NaOH (190oC, 5min), but the yield of mannan was very low (3%). Impregnation with 0.025% NaOH and heat-fractionation at 190oC for 5min resulted in extraction of GGM with MW of 9500 and a mannan yield of 31%. When the spruce chips were impregnated with =<0.05% NaOH an O-acetyl-galactoglucomannan was extracted, whereas when higher NaOH charges were used in the impregnation, the extracted GGM lacked acetyl groups.}}, author = {{Lundqvist, Jon and Jacobs, Anna and Palm, Magnus and Zacchi, Guido and Dahlman, Olof and Stålbrand, Henrik}}, issn = {{0144-8617}}, keywords = {{Spruce (Picea abies); Hemicellulose; O-acetyl-galactoglucomannan; Microwave oven; Size-exclusion chromatography; MALDI-MS}}, language = {{eng}}, number = {{2}}, pages = {{203--211}}, publisher = {{Elsevier}}, series = {{Carbohydrate Polymers}}, title = {{Characterization of galactoglucomannan extracted from spruce (Picea abies) by heat-fractionation at different conditions}}, url = {{http://dx.doi.org/10.1016/S0144-8617(02)00111-X}}, doi = {{10.1016/S0144-8617(02)00111-X}}, volume = {{51}}, year = {{2003}}, }