Retrograde axonal transport of locally synthesized proteins, e.g., actin and heat shock protein 70, in regenerating adult frog sciatic sensory axons
(1994) In Journal of Neuroscience Research 38(4). p.424-432- Abstract
The local synthesis and subsequent retrograde axonal transport of [35S]methionine‐labelled proteins was studied in the in vitro regenerating adult frog sciatic sensory axons. By the use of a three compartment culture system, proteins in the outgrowth region were selectively labelled. After 2 days in culture a rise in TCA‐insoluble radioactivity was detected in the dorsal root ganglia, which could be prevented by the addition of viblastine or 2,4‐dinitrophenol to the nerve proximal to the crush site. Two‐dimensional polyacrylamide gel electrophoresis of ganglionic proteins revealed a pattern of 35 labelled polypeptides with apparent molecular masses (Mm) ranging from < 15 to 95 kDa and with isoelectric points... (More)
The local synthesis and subsequent retrograde axonal transport of [35S]methionine‐labelled proteins was studied in the in vitro regenerating adult frog sciatic sensory axons. By the use of a three compartment culture system, proteins in the outgrowth region were selectively labelled. After 2 days in culture a rise in TCA‐insoluble radioactivity was detected in the dorsal root ganglia, which could be prevented by the addition of viblastine or 2,4‐dinitrophenol to the nerve proximal to the crush site. Two‐dimensional polyacrylamide gel electrophoresis of ganglionic proteins revealed a pattern of 35 labelled polypeptides with apparent molecular masses (Mm) ranging from < 15 to 95 kDa and with isoelectric points (pI) ranging from 4.5 to 6.5. The major ones, representing about 75% of the activity in a gel, were of Mm/pI 47/5.4, 48/6.1,
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- author
- Edbladh, M. LU ; Ekström, P. A R and Edström, A. LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- electrophoresis, heat shock protein, injury, nerve, Schwann cell
- in
- Journal of Neuroscience Research
- volume
- 38
- issue
- 4
- pages
- 9 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:7523692
- scopus:0028340597
- ISSN
- 0360-4012
- DOI
- 10.1002/jnr.490380408
- language
- English
- LU publication?
- yes
- id
- bd6964b6-a5f5-42d7-9596-55406d1cb3cf
- date added to LUP
- 2016-12-07 14:50:56
- date last changed
- 2025-01-12 17:09:47
@article{bd6964b6-a5f5-42d7-9596-55406d1cb3cf,
abstract = {{<p>The local synthesis and subsequent retrograde axonal transport of [<sup>35</sup>S]methionine‐labelled proteins was studied in the in vitro regenerating adult frog sciatic sensory axons. By the use of a three compartment culture system, proteins in the outgrowth region were selectively labelled. After 2 days in culture a rise in TCA‐insoluble radioactivity was detected in the dorsal root ganglia, which could be prevented by the addition of viblastine or 2,4‐dinitrophenol to the nerve proximal to the crush site. Two‐dimensional polyacrylamide gel electrophoresis of ganglionic proteins revealed a pattern of 35 labelled polypeptides with apparent molecular masses (M<sub>m</sub>) ranging from < 15 to 95 kDa and with isoelectric points (pI) ranging from 4.5 to 6.5. The major ones, representing about 75% of the activity in a gel, were of M<sub>m</sub>/pI 47/5.4, 48/6.1,</p>}},
author = {{Edbladh, M. and Ekström, P. A R and Edström, A.}},
issn = {{0360-4012}},
keywords = {{electrophoresis; heat shock protein; injury; nerve; Schwann cell}},
language = {{eng}},
number = {{4}},
pages = {{424--432}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Journal of Neuroscience Research}},
title = {{Retrograde axonal transport of locally synthesized proteins, e.g., actin and heat shock protein 70, in regenerating adult frog sciatic sensory axons}},
url = {{http://dx.doi.org/10.1002/jnr.490380408}},
doi = {{10.1002/jnr.490380408}},
volume = {{38}},
year = {{1994}},
}