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Retrograde axonal transport of locally synthesized proteins, e.g., actin and heat shock protein 70, in regenerating adult frog sciatic sensory axons

Edbladh, M. LU ; Ekström, P. A R and Edström, A. LU (1994) In Journal of Neuroscience Research 38(4). p.424-432
Abstract

The local synthesis and subsequent retrograde axonal transport of [35S]methionine‐labelled proteins was studied in the in vitro regenerating adult frog sciatic sensory axons. By the use of a three compartment culture system, proteins in the outgrowth region were selectively labelled. After 2 days in culture a rise in TCA‐insoluble radioactivity was detected in the dorsal root ganglia, which could be prevented by the addition of viblastine or 2,4‐dinitrophenol to the nerve proximal to the crush site. Two‐dimensional polyacrylamide gel electrophoresis of ganglionic proteins revealed a pattern of 35 labelled polypeptides with apparent molecular masses (Mm) ranging from < 15 to 95 kDa and with isoelectric points... (More)

The local synthesis and subsequent retrograde axonal transport of [35S]methionine‐labelled proteins was studied in the in vitro regenerating adult frog sciatic sensory axons. By the use of a three compartment culture system, proteins in the outgrowth region were selectively labelled. After 2 days in culture a rise in TCA‐insoluble radioactivity was detected in the dorsal root ganglia, which could be prevented by the addition of viblastine or 2,4‐dinitrophenol to the nerve proximal to the crush site. Two‐dimensional polyacrylamide gel electrophoresis of ganglionic proteins revealed a pattern of 35 labelled polypeptides with apparent molecular masses (Mm) ranging from < 15 to 95 kDa and with isoelectric points (pI) ranging from 4.5 to 6.5. The major ones, representing about 75% of the activity in a gel, were of Mm/pI 47/5.4, 48/6.1,

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
electrophoresis, heat shock protein, injury, nerve, Schwann cell
in
Journal of Neuroscience Research
volume
38
issue
4
pages
9 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • pmid:7523692
  • scopus:0028340597
ISSN
0360-4012
DOI
10.1002/jnr.490380408
language
English
LU publication?
yes
id
bd6964b6-a5f5-42d7-9596-55406d1cb3cf
date added to LUP
2016-12-07 14:50:56
date last changed
2024-01-19 14:46:01
@article{bd6964b6-a5f5-42d7-9596-55406d1cb3cf,
  abstract     = {{<p>The local synthesis and subsequent retrograde axonal transport of [<sup>35</sup>S]methionine‐labelled proteins was studied in the in vitro regenerating adult frog sciatic sensory axons. By the use of a three compartment culture system, proteins in the outgrowth region were selectively labelled. After 2 days in culture a rise in TCA‐insoluble radioactivity was detected in the dorsal root ganglia, which could be prevented by the addition of viblastine or 2,4‐dinitrophenol to the nerve proximal to the crush site. Two‐dimensional polyacrylamide gel electrophoresis of ganglionic proteins revealed a pattern of 35 labelled polypeptides with apparent molecular masses (M<sub>m</sub>) ranging from &lt; 15 to 95 kDa and with isoelectric points (pI) ranging from 4.5 to 6.5. The major ones, representing about 75% of the activity in a gel, were of M<sub>m</sub>/pI 47/5.4, 48/6.1,</p>}},
  author       = {{Edbladh, M. and Ekström, P. A R and Edström, A.}},
  issn         = {{0360-4012}},
  keywords     = {{electrophoresis; heat shock protein; injury; nerve; Schwann cell}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{424--432}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Journal of Neuroscience Research}},
  title        = {{Retrograde axonal transport of locally synthesized proteins, e.g., actin and heat shock protein 70, in regenerating adult frog sciatic sensory axons}},
  url          = {{http://dx.doi.org/10.1002/jnr.490380408}},
  doi          = {{10.1002/jnr.490380408}},
  volume       = {{38}},
  year         = {{1994}},
}